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Alexa fluor 647 conjugated phosphorylated ser28 histone h3 antibody

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Alexa Fluor 647-conjugated phosphorylated (Ser28) histone H3 antibody is a fluorescently labeled antibody that specifically recognizes histone H3 phosphorylated at serine 28. This antibody can be used to detect and analyze histone H3 phosphorylation in various applications.

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Lab products found in correlation

Fixation permeabilization solution, by BD (2 mentions) Perm wash buffer, by BD (2 mentions)

Spelling variants of this product

Alexa Fluor 647 (65 citations) Alexa 647 (11 citations) Alexa Fluor 647–conjugated antibody (2 citations)

2 protocols using alexa fluor 647 conjugated phosphorylated ser28 histone h3 antibody

1

Cell Cycle Synchronization and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Where indicated, cells were treated for at least 20 h with RO-3306 (10 μM) in the culture medium to synchronize cells at the G2/M phase border, and then released from the RO-3306-imposed cell cycle block for different time periods by replacing with fresh culture medium.
Cells were collected and fixed in Fixation/Permeabilization solution (BD Biosciences) for 20 min at 4°C, followed by staining with Alexa Fluor 647-conjugated phosphorylated (Ser28) histone H3 antibody (BioLegend) in 50 μl of BD Perm/Wash buffer (BD Biosciences). Cells were analyzed by flow cytometry or sorted before immunoprecipitation and immunoblot analysis.
Shi H., Wang Y., Li X., Zhan X., Tan M., Fina M., Su L., Pratt D., Bu C.H., Hildebrand S., Lyon S., Scott L., Quan J., Sun Q., Russell J., Arnett S., Jurek P., Chen D., Kravchenko V.V., Mathison J.C., Moresco E.M., Monson N.L., Ulevitch R.J, & Beutler B. (2015). NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Nature immunology, 17(3), 250-258.
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2

Cell Cycle Synchronization and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Where indicated, cells were treated for at least 20 h with RO-3306 (10 μM) in the culture medium to synchronize cells at the G2/M phase border, and then released from the RO-3306-imposed cell cycle block for different time periods by replacing with fresh culture medium.
Cells were collected and fixed in Fixation/Permeabilization solution (BD Biosciences) for 20 min at 4°C, followed by staining with Alexa Fluor 647-conjugated phosphorylated (Ser28) histone H3 antibody (BioLegend) in 50 μl of BD Perm/Wash buffer (BD Biosciences). Cells were analyzed by flow cytometry or sorted before immunoprecipitation and immunoblot analysis.
Shi H., Wang Y., Li X., Zhan X., Tan M., Fina M., Su L., Pratt D., Bu C.H., Hildebrand S., Lyon S., Scott L., Quan J., Sun Q., Russell J., Arnett S., Jurek P., Chen D., Kravchenko V.V., Mathison J.C., Moresco E.M., Monson N.L., Ulevitch R.J, & Beutler B. (2015). NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Nature immunology, 17(3), 250-258.
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