Irdye 680lt goat anti rat igg

Manufactured by LI COR

IRDye 680LT goat anti-rat IgG is a near-infrared fluorescent secondary antibody that specifically binds to rat immunoglobulin G (IgG). It is designed for use in immunoassays, Western blotting, and other applications that require the detection of rat IgG.

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Lab products found in correlation

Irdye 800cw goat anti rabbit igg, by LI COR (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Complete edta free, by Merck Group (1 mentions) Anti ha, by Roche (1 mentions) Odyssey clx, by LI COR (1 mentions) Ecl western blotting detection reagent, by Cytiva (1 mentions) β actin, by Novus Biologicals (1 mentions) Irdye 680rd donkey anti mouse igg, by LI COR (1 mentions) Cldn5, by Thermo Fisher Scientific (1 mentions) Sds page gel, by Thermo Fisher Scientific (1 mentions) Tissuelyser 2, by Qiagen (1 mentions) Complete protease inhibitor cocktail tablet, by Roche (1 mentions) Phosstop phosphatase inhibitor cocktail tablet, by Roche (1 mentions)

3 protocols using irdye 680lt goat anti rat igg

Western Blot Analysis of P. falciparum

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Schizonts were released from erythrocytes by the addition of phosphate-buffered saline (PBS) containing 0.15% (wt/vol) saponin and protease inhibitors (cOmplete EDTA-free; Sigma) for 3 min on ice. Saponin lysates were solubilized with 3× sample buffer with 5% beta-mercaptoethanol at a concentration of 1.6 × 10⁹ parasites/ml. Parasite extracts were subjected to SDS-PAGE and transferred to a nitrocellulose membrane. Membranes were immunostained with rat antihemagglutinin (anti-HA) (1:1,000 dilution; Roche) and rabbit anti-P. falciparum EBA175 (anti-PfEBA175) (1:2,000 dilution; a kind gift from Christine R. Collins), followed by IRDye 680LT goat anti-rat IgG (1:10,000 dilution; LI-COR) and IRDye 800CW goat anti-rabbit IgG (1:10,000 dilution; LI-COR). The signals were detected by a fluorescence imager (Odyssey CLx; LI-COR).

Tibúrcio M., Yang A.S., Yahata K., Suárez-Cortés P., Belda H., Baumgarten S., van de Vegte-Bolmer M., van Gemert G.J., van Waardenburg Y., Levashina E.A., Sauerwein R.W, & Treeck M. (2019). A Novel Tool for the Generation of Conditional Knockouts To Study Gene Function across the Plasmodium falciparum Life Cycle. mBio, 10(5), e01170-19.

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Western Blot Analysis of Parasite Proteins

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Parasite extracts were solubilized in protein loading buffer, denatured at 95°C for 10 min, subjected to SDS-PAGE, and transferred onto a nitrocellulose membrane. Membranes were immunostained with mouse anti-GFP (1:250 dilution; Roche, 11814460001), rabbit anti-Aldolase-horseradish peroxidase (HRP) conjugated (1:5,000 dilution; Abcam ab38905), and rabbit anti-histone 3 (1:2,000 dilution; Abcam ab1791) primary antibodies. Antibody detection was done using chemiluminescent Western blotting using goat anti-mouse secondary antibody conjugated with HRP and the ECL Western blotting detection reagents (Amersham RPN2106) or by direct infrared fluorescence detection on the Odyssey Infrared Imaging System (Odyssey CLx, LI-COR) using IRDye 680LT goat anti-rat IgG (1:10,000 dilution; LI-COR) and IRDye 800CW goat anti-rabbit IgG (1:10,000 dilution; LI-COR).

Tibúrcio M., Hitz E., Niederwieser I., Kelly G., Davies H., Doerig C., Billker O., Voss T.S, & Treeck M. (2021). A 39-Amino-Acid C-Terminal Truncation of GDV1 Disrupts Sexual Commitment in Plasmodium falciparum. mSphere, 6(3), e01093-20.

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Western Blot Analysis of Lung Tissue

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P22 lung tissues were collected in RIPA buffer supplemented with Complete Protease Inhibitor Cocktail tablets (Roche) and PhosSTOP Phosphatase Inhibitor Cocktail tablets (Roche). Protein samples were then homogenized in a QIAGEN TissueLyser II. Protein concentrations were measured by Pierce™ BCA Protein Assay kit (Thermo Scientific).
An estimated ~10ug protein sample was loaded in each well and ran on a 4%–12% SDS-PAGE gel (Invitrogen), followed by transfer to PVDF membranes. PVDF membranes were blocked in TBST (0.1% Tween-20) with 5% BSA for 1.5 hours, incubated in primary antibodies overnight, and then incubated in the secondary antibody for 1 hour. All imaging and quantification were performed using the Image Studio Lite software system (LI-COR Biosciences). The following primary antibodies were used: CLDN5 (Invitrogen, 4C3C2, 1:2000), CDH5 (BD, 11D4.1, 1:1500) and β-actin (Novus Biologicals, NB600501, 1:5000). The secondary antibody used were: IRDye 680RD donkey anti–mouse IgG (LI-COR, 925–69072, 1:10,000) and IRDye 680LT goat anti-rat IgG (LI-COR, 925–68029, 1:10,000). Three biological replicates were performed for each antibody.

Xu J., Xu L., Sui P., Chen J., Moya E.A., Hume P., Janssen W.J., Duran J.M., Thistlethwaite P., Carlin A., Gulleman P., Banaschewski B., Goldy M.K., Yuan J.X., Malhotra A., Pryhuber G., Crotty-Alexander L., Deutsch G., Young L.R, & Sun X. (2022). Excess Neuropeptides in Lung Signal through Endothelial Cells to Impair Gas Exchange. Developmental cell, 57(7), 839-853.e6.

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