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Lm 10hs

Manufactured by Malvern Panalytical
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The LM-10HS is a laser diffraction analyzer for particle size analysis. It is designed to measure the particle size distribution of materials in the range of 0.1 to 3500 microns. The instrument uses a laser beam to illuminate the sample and measures the angular variation in the intensity of the scattered light to determine the particle size distribution.

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Lab products found in correlation

Bca protein assay, by Thermo Fisher Scientific (2 mentions) 405 nm laser, by Malvern Panalytical (1 mentions) Nano z, by Malvern Panalytical (1 mentions)

4 protocols using lm 10hs

1

Characterizing Extracellular Vesicle Purity

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The number of vesicles recovered was determined by Nanoparticle Tracking Analysis (NTA) on a Nanosight LM-10HS equipped with a 405 nm laser (Malvern) using the manufacturer's instructions. Resuspended vesicles were diluted 1:40 to 1:200 with PBS before analysis. The purity of the EV isolated were assessed using transmission electron microscopy (TEM) as previously described [31 (link)]. Qualitative assessment of TEM was used to gauge the abundance of protein aggregates.
Akers J.C., Ramakrishnan V., Kim R., Phillips S., Kaimal V., Mao Y., Hua W., Yang I., Fu C.C., Nolan J., Nakano I., Yang Y., Beaulieu M., Carter B.S, & Chen C.C. (2015). miRNA contents of cerebrospinal fluid extracellular vesicles in glioblastoma patients. Journal of neuro-oncology, 123(2), 205-216.
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2

Nanoparticle Tracking Analysis of Extracellular Vesicles

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The number of vesicles recovered was determined by Nanoparticle Tracking Analysis (NTA) on a Nanosight LM-10HS equipped with a 405nm laser (Malvern) that was calibrated with polystyrene latex microbeads at 100 nm and 200 nm prior to analysis. Resuspended vesicles were diluted with PBS to achieve between 20–100 objects per frame. EVs were manually injected into the sample chamber at ambient temperature. Each sample was measured in triplicate at camera setting 13 with acquisition time of 30 s and detection threshold setting of 7. At least 200 completed tracks were analyzed per video. The NTA analytical software version 2.3 was used for capturing and analyzing the data.
The morphology of the EV isolated were assessed using transmission electron microscopy (TEM) as previously described [15 ].
Akers J.C., Ramakrishnan V., Yang I., Hua W., Mao Y., Carter B.S, & Chen C.C. (2016). Optimizing Preservation of Extracellular Vesicular miRNAs Derived from Clinical Cerebrospinal Fluid. Cancer biomarkers : section A of Disease markers, 17(2), 125-132.
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3

Exosome Characterization via DLS, NTA, and BCA

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Exosome size was determined using DLS on the Zetasizer Nano ZS (Malvern) using the number function. Nano tracking analysis (NTA) was performed using the NanoSight LM10-HS (Malvern) at the Northwestern University Keck Biophysics Facility. Exosomal protein concentration was determined via BCA Protein Assay (Thermo Scientific, 23227). Gold and rhodamine content was observed using UV-Vis spectroscopy and absorbance readings at 520 nm and 560 nm, respectively.
Angeloni N.L., McMahon K.M., Swaminathan S., Plebanek M.P., Osman I., Volpert O.V, & Thaxton C.S. (2016). Pathways for Modulating Exosome Lipids Identified By High-Density Lipoprotein-Like Nanoparticle Binding to Scavenger Receptor Type B-1. Scientific Reports, 6, 22915.
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4

Preparation and Crystallization of 30S Ribosomal Subunits

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30S ribosomal subunits from T. thermophilus HB8 (ATCC27634) (20 (link)) were prepared as previously described (12 (link),21 (link)). Purified 30S ribosomal subunits were crystallized at 4°C by the hanging drop method using a mother liquor solution containing 17% (v/v) MPD, 15 mM magnesium acetate, 200 mM potassium acetate, 75 mM ammonium acetate and 100 mM 2-(N-morpholino) ethanesulfonic acid (MES)-KOH (pH 6.5). Microcrystals 2–3 × 2–3 × 3–4 μm3 in size were harvested in the same mother liquor composition, pooled (total volume of 500 μl loaded) and supplemented with 200 μM of each mRNA oligos, ASLPhe and 100 μg/ml sisomicin parent compound or N1MS derivative compound 48 h before data collection. Crystal concentration was approximated to be 1010–1011 particles per ml based on light microscopy and nanoparticle tracking analysis (NanoSight LM10-HS with corresponding Nanoparticle Tracking Analysis (NTA) software suite (Malvern Instruments, Malvern, UK).
O’Sullivan M.E., Poitevin F., Sierra R.G., Gati C., Dao E.H., Rao Y., Aksit F., Ciftci H., Corsepius N., Greenhouse R., Hayes B., Hunter M.S., Liang M., McGurk A., Mbgam P., Obrinsky T., Pardo-Avila F., Seaberg M.H., Cheng A.G., Ricci A.J, & DeMirci H. (2018). Aminoglycoside ribosome interactions reveal novel conformational states at ambient temperature. Nucleic Acids Research, 46(18), 9793-9804.
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