Human recombinant ifn γ

VSL#3 packets with 450 billion bacteria per sachet (gift from Professor Claudio De Simone), human recombinant IFN-γ (Roche, Mannheim, Germany), cycloheximide (Sigma-Aldrich, St. Louis, MO, USA), monoclonal mouse anti-TCPTP antibody CF-4, which detects the 45-kilodalton and the 48-kilodalton isoforms (Calbiochem, San Diego, CA), mouse anti-TCPTP (Ab-1) antibody (EMD Millipore, Billerica, MA), anti-phospho-STAT1 (Tyr⁷⁰¹), anti-STAT1, (Cell Signaling Technologies, Danvers, MA), Claudin-2, Occludin and ZO-1 (Invitrogen, Waltham, Massachusetts, USA) and monoclonal mouse anti-β-Actin (Sigma) were obtained from the sources noted. Millicell culture plate inserts were purchased from Millipore Corporation (Millipore, Bedford, MA). McCoy’s 5A and DMEM media were purchased form Corning Inc, (Corning, NY). All other reagents were of analytical grade and acquired commercially.

Human recombinant IFN-γ (Roche, Mannheim, Germany), monoclonal mouse anti-β-Actin (Sigma-Aldrich, St. Louis, MO), monoclonal mouse anti-TCPTP antibody CF-4, which detects the 45-kD and 48-kD isoforms (Millipore, Billerica, MA), anti-phospho-STAT1 (Tyr⁷⁰¹), anti-STAT1, (Cell Signaling Technologies, Danvers, MA), anti-claudin-2, anti-occludin, anti-CDX2, and anti-ZO-1 antibodies (Invitrogen, Waltham, MA) were obtained from the sources noted. Millicell culture plate inserts were purchased from Millipore Corporation (Millipore, Bedford, MA). McCoy’s 5A and DMEM media were purchased form Corning Inc., (Corning, NY). The ON-TARGET plus SMART pool siRNA and ON-TARGET plus Non-targeting pool were purchased from GE Dharmacon (Pittsburgh, PA). All other reagents were of analytical grade and acquired commercially.