C anp4 23

A ring‐deleted analog of ANP; C‐ANP_4‐23 was purchased from Bachem (Torrance, CA). Leucine, L‐(4,5‐3H(N)) was purchased from Perkin Elmer (Boston, MA). Polyclonal AT‐1 (N‐10), Monoclonal Gqα antibody (10), monoclonal PLC‐β1 antibody (D‐8), monoclonal (phosphor)‐ERK1/2 (phospho‐specific‐tyrosine204) antibody, polyclonal ERK1/2 antibody (C‐14), monoclonal dynein IC1/2 antibody (74‐1), Polyclonal EGFR, IGF‐1R (phospho)‐c‐Src (phospho‐specific‐tyrosine‐419), PDGFR and (phospho)‐IGF‐1R (phospho‐specific‐tyrosine1165/1166) antibodies, and Western blotting reagents were purchased from St Cruz Biotech (Santa Cruz, CA, USA). Polyclonal (phospho)‐EGFR antibody (phospho‐specific‐tyrosine‐1173), polyclonal (phospho)‐PDGFR (phospho‐specific‐tyrosine 857) were purchased from Cell Signaling Technology (Danvers, MA, USA). Gq inhibitor (GqI), YM‐254890 was purchased from Wako Inc (Osaca, Japan).

C-ANP _4–23 was purchased from Bachem, antibodies against Cyclin D1, Cdk4, total EGF-R, phospho-EGFR, total PDGF-R β, phospho-PDGF-R β, total IGF-R β, phospho-IGF-IR, c-Src, phosphor-c-Src, horseradish peroxidase-conjugated goat anti-mouse/anti-rabbit and anti-goat immunoglobulin were from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA), antibodies against Nox1, Nox2, Nox4 and p47^phox were from EMD Millipore. Thymidine, [Methyl-3H] was from PerkinElmer, Inc. (Massachusetts, U.S.A.). All other chemicals were purchased from Sigma Aldrich Canada.