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L 6000

Manufactured by Hitachi
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Sourced in Japan

The L-6000 is a piece of laboratory equipment manufactured by Hitachi. It is a high-performance instrument designed for analytical purposes. The core function of the L-6000 is to perform accurate and precise measurements and analyses. Further details on the intended use or specific applications of this product are not available.

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Lab products found in correlation

Lichrospher 100 rp 18 column, by Merck Group (1 mentions) Model spectrum 100, by PerkinElmer (1 mentions) Al 400, by JEOL (1 mentions) Eca 600, by JEOL (1 mentions) P 2300, by Jasco (1 mentions) Model jms 700, by JEOL (1 mentions)

3 protocols using l 6000

1

ACE Inhibition Assay Protocol

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Inhibition of ACE was determined according to the method described by Cushman and Cheung [22 (link)] with modifications. After incubating 45-μL Hip-His-Leu (3mM in pH 8.3 sodium borate buffer) and 10-μL sample or lisinopril (used as the standard) at 37°C for 10 minutes, 45-μL ACE (33 mU/mL in pH 8.3 sodium borate buffer) was added to react for 30 minutes, and then 150-μL TFA (0.1%) was added to terminate the reaction. While Hip-His-Leu was converted into hippuric acid (HA) and histidylleucine by ACE, HA concentration was determined using a high performance liquid chromatographer (L-6000, Hitachi, Tokyo, Japan) equipped with a Lichrospher 100 RP-18 column (Merck, Darmstadt, Germany). The methanol containing 0.1% TFA as an eluting solvent was at a flow rate of 0.6 mL/min and HA was detected at 228 nm. All of these determinations were repeated three times.
ACE inhibition was calculated according to the following equation:
where AC and AS were the peak areas of buffer (control) or sample, respectively.
Chen P.H., Weng Y.M., Yu Z.R., Koo M, & Wang B.J. (2016). Extraction temperature affects the activities of antioxidation, carbohydrate-digestion enzymes, and angiotensin-converting enzyme of Pleurotus citrinopileatus extract. Journal of Food and Drug Analysis, 24(3), 548-555.
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2

Spectroscopic Analysis of Compounds

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Melting points were measured on a Yanagimoto micro apparatus without correction. Measurement of optical rotation was carried out on a JASCO P-2300 in CHCl3 solution at 23 °C. IR spectra were recorded using a Perkin-Elmer Model Spectrum 100. 1H (400 or 600 MHz)- and 13C (100 or 150 MHz)-NMR spectra were recorded on a JEOL AL-400 or ECA-600 spectrometer using tetramethylsilane as an internal standard. The chemical shifts are expressed on the δ scale. EI-MS and HREI-MS were measured at 30–60 eV (direct inlet) with a JEOL Model JMS-700 and the relative intensities of peaks are reported with reference to the most intense peak higher than m/z 100. TLC was carried out on pre-coated silica gel 60 (Merck 5554) with n-hexane-EtOAc or CHCl3-MeOH as the developing phase. Detection was carried out by spraying with concentrated H2SO4 followed by heating. HPLC was performed on a Hitachi L-6000 equipped with a Hitachi L-7490 RI detector. The following column and solvents were used for elution: Mightysil, RP-18 GP 250–10 (25 cm x 10 mm i.d., 5μm) with MeOH-H2O or CH3CN-H2O.
Yamashita H., Matsuzaki M., Kurokawa Y., Nakane T., Goto M., Lee K.H., Shibata T., Bando H, & Wada K. (2019). Four New Triterpenoids from the Bark of Euonymus alatus forma ciliato-dentatus. Phytochemistry letters, 31, 140-146.
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3

Amperometric Measurement of Hydrogen

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Amperometric measurements of H2 were performed using an electrochemical measurement apparatus in connection with a potentiostat (NPOT-2501, Nikko Keisoku Corp., Japan), and a digital recorder (GR-3500, KEYENCE, Japan). The FIA system consisted of a carrier reservoir, sample injector with 10 μL loop, interconnecting PTFE tubing, HPLC pump (L-6000, Hitachi, Japan) and the electrochemical detector. A working Pt-NGC electrode (6 mm diameter, BAS Inc., Japan), an aqueous Ag/ AgCl (3 M NaCl electrolyte) reference and a platinum counter electrode were integrated on a radial flow cell (BAS Inc., Japan). Phosphate buffer (0.10 M, pH 7.0) was used as the carrier solution. The test solution used was 0.1 M phosphate buffer (pH 7.0) to which the bubbling of the H2 gas was performed.
Matsuura H., Takahashi T., Sakamoto S., Kitamura T, & Uchiyama S. (2017). An Amperometric Flow Injection Analysis of Dissolved Hydrogen Molecule Using Tightly Immobilized Electrodeposited Platinum Particles on Nitrogen-containing Functional Groups Introduced Glassy Carbon Electrodes. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 33(6).
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