Antibodies were obtained from the following sources; anti-MAP2, anti-beta-tubulin, and anti-synaptophysin from Cell Signaling Inc. (Beverly, MA), β-tubulin from LI-COR, Odyssey (Lincoln, NE), anti-SRSF1 from Invitrogen, anti-SRSF2 from Sigma Aldrich, anti-MCL1, anti-SRSF3, anti-SRSF4, and anti-HNRNP A1 from Santa Cruz Biotech, and anti-Grb2 from BD Biosciences. Mammalian protease inhibitors were obtained from Sigma-Aldrich (St Louis, MO). Bradford reagent was from BioWorld (Dublin, OH). MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) was obtained from Thermo Fisher Scientific (#M6494).
Anti srsf3
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-SRSF3 is a laboratory reagent used to detect and quantify the presence of SRSF3 protein in biological samples. It is a highly specific antibody that binds to the SRSF3 protein, allowing for its identification and measurement through various immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Anti mcl 1, by Santa Cruz Biotechnology (1 mentions)
Anti synaptophysin, by Cell Signaling Technology (1 mentions)
Anti grb2, by BD (1 mentions)
Anti srsf2, by Merck Group (1 mentions)
Anti map2, by Cell Signaling Technology (1 mentions)
Anti beta tubulin, by Cell Signaling Technology (1 mentions)
β tubulin, by LI COR (1 mentions)
Anti srsf1, by Thermo Fisher Scientific (1 mentions)
Anti hnrnp a1, by Santa Cruz Biotechnology (1 mentions)
Mammalian protease inhibitor, by Merck Group (1 mentions)
Bradford reagent, by Bioworld Technology (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Anti hnrnp c, by Santa Cruz Biotechnology (1 mentions)
Anti ki67, by Abcam (1 mentions)
Nitrocellulose membrane, by Pall Corporation (1 mentions)
2 protocols using anti srsf3
1
Antibody Sources and Reagents
2
Protein Expression Analysis Protocol
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Total cellular protein samples were prepared by lysing cells with 2 × SDS sample buffer, and samples were denatured for 3 min at 95 °C, then separated in a 10% SDS-PAGE gel (GeneScript, Nanjing, China), followed by being transferred to a nitrocellulose membrane (Pall Corporation, Port Washington, NY, USA). Membranes were blocked with 5% skim milk and incubated with the following antibodies: anti-β-actin (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-47778), anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-47724), anti-HNRNPC (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-32308), anti-SRSF3 (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-13510), anti-Ki67 (Abcam, Cambridge, UK, Cat# ab16667), and anti-AKR1C2 (ABclonal, Wuhan, China, Cat# A1048).
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