Ab33516

The following antibodies were used in this study: CBFβ rabbit polyclonal (ab33516, Abcam, Cambridge, MA, USA) and A303-547A, A303-548A, A303-549A, Bethyl Labs, Montgomery, TX, USA). Dilutions for ab33516 were IF (1:500) and WB (1:500). Dilutions and concentration used for CBFβ Bethyl antibodies were IF (1:1000), IP (5 µg) and WB (1:1,000). GFP rabbit polyclonal (ab290, Abcam, Cambridge, MA, USA), was used at dilutions for WB (1:1000) and IP (5 µl). RUNX2 (8G5) mouse monoclonal (MBL International, Woburn, MA, USA), dilution used for IF (1:600). Filamin A (EP2405Y) rabbit polyclonal (ab76289, Abcam, Cambridge, MA, USA) dilution was used for IF (1:1000). Beta-tubulin mouse monoclonal (T-4026, Sigma Aldrich, St. Louis, MO, USA), dilution used for IF (1:1000) and WB (1:1000). PRC1 goat polyclonal (K-18) (sc-9342, Santa Cruz Biotechnology, Santa Cruz, CA, USA), dilution used for IF (1:300). KIF4A goat polyclonal (ab3815, Abcam, Cambridge, MA, USA), dilution used for IF (1:300). MRLC3 goat polyclonal (sc9449, Santa Cruz Biotechnology, Santa Cruz, CA, USA), dilution used for WB (1:200). Secondary antibodies conjugated with HRP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), dilution used for WB (1:5000). Secondary antibodies conjugated with Alexa fluor 488 or 594 (Life Technologies-Invitrogen, Carlsbad, CA, USA), were used at dilution IF (1:500).

The following antibodies were used in this study: CBFβ rabbit polyclonal (ab33516, Abcam, Cambridge, MA), dilutions were 1:500 (IF, WB), 1:500 and 5 μg per IP (ChIP); CBFβ rabbit polyclonal (A-303-547A, Bethyl Labs, Montgomery, TX), dilutions were 1:1000 (WB) and 5 μg per IP; Runx1 (Cell Signaling Technology, Boston, MA), dilutions were 1:50 (IF) and 1:1000 (WB ); RUNX2 mouse monoclonal (8G5, MBL International, Woburn, MA), dilutions were 1:600 (IF) and 1:1000 (WB); UBF (F-9, Santa Cruz Biotechnology, Dallas, TX), dilutions were 1:500 (IF, WB); HDAC1 (H-51, Santa Cruz Biotechnology), 1:1000 dilution used for WB; beta-tubulin mouse monoclonal (T-4026, Sigma Aldrich, St. Louis, MO), 1:1000 dilution used for WB; lamin B1 (ab16048, Abcam) 1:1000 dilution used for WB; Normal IgG control (source) was used at 5 μg for IP. Secondary antibodies conjugated with HRP (Santa Cruz Biotechnology), were used at a dilution of 1:5000 for WB. Secondary antibodies conjugated with Alexa Fluor 488 or 594 (Life Technologies) were used at dilution of 1:500 for IF.