LSM 700 (Carl Zeiss) confocal microscope, LCI Plan-Neofluar 63×/1.30 glycerol immersion objective at RT, and Zen Black acquisition software (Carl Zeiss) were used for confocal microscopy image acquisition. A combination of Zen Blue Lite (Carl Zeiss), and CorelDraw programs were used for image processing.
Zen blue lite
Manufactured by Zeiss
Sourced in Germany
Zen Blue Lite is a software package for microscope control and image acquisition. It provides a user-friendly interface for configuring and operating Zeiss microscopes. The software enables basic image capture and basic processing functions.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 700, by Zeiss (3 mentions)
Zen black acquisition software, by Zeiss (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Lsm 800, by Zeiss (2 mentions)
Sir lysosome, by Cytoskeleton (2 mentions)
Lsm 880, by Zeiss (1 mentions)
Coreldraw, by Corel (1 mentions)
Photo paint 2017, by Corel (1 mentions)
Axiocam 506, by Zeiss (1 mentions)
Axio imager, by Zeiss (1 mentions)
Plan neofluar, by Zeiss (1 mentions)
9 protocols using zen blue lite
1
Confocal Microscopy Imaging Protocol
2
Subcellular Localization of ARG2 Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To determine the subcellular localization of ARG2, full‐length ARG2 coding sequence amplified from SC328C and cloned by Gateway (Invitrogen, Waltham, MA, USA) recombination reactions into the pEarleyGate103 vector, upstream of the mGFP5 under the control of the CaMV 35 S promoter. Transient protein expression assays were performed as previously described (Helm et al., 2019 (link)). Bacterial suspensions were mixed in equal ratios (1:1) and infiltrated into the 3‐week‐old Nb leaves. Confocal laser scanning microscopy was performed at 48 h following agroinfiltration using a LSM880 (Carl Zeiss, Jena, Germany) upright confocal microscope. Leaf sections were examined on abaxial surface, and at least two different leaves from two different plants were imaged. mGFP protein fusions were excited using a 488‐nm argon laser and detected between 525 nm and 550 nm. mCherry fusions were excited using a 561 nm helium‐neon laser and detected at 610 nm. Confocal images were collected and processed using zen blue lite (Carl Zeiss).
3
Confocal Microscopy Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All images were taken using the LSM 700 (Carl Zeiss) confocal microscope, LCI Plan-Neofluar 63×/1.30 glycerol immersion objective at room temperature, and Zen Black acquisition software (Carl Zeiss AG). Image analysis was done using the Zen Blue Lite (Carl Zeiss), PHOTO-PAINT and CorelDraw programs from the CorelDraw Graphics Suite 2017. Postimaging processing was done using Corel PHOTO-PAINT 2017 using the brightness/contrast/intensity adjustment settings equally for images from the same imaging series.
4
Quantification of TRAF6 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The sections were photographed Carl Zeiss Image (CZI) under exactly the same conditions, and images were processed uniformly with Zen Blue Lite (ZEN 2.3(blue edition), Carl Zeiss Microscopy Gmbh,2011). The semiquantitative analysis, calculation of MOD value of TRAF6, was carried out by ImageJ software (ImageJ 1.52a, Wayne Rasband), and the average value was calculated 3 times for each sample.
5
Confocal Imaging and Post-Processing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All microscopy images were taken using the LSM 700 (Carl Zeiss) confocal microscope, LCI Plan-Neofluar 63x/1.30 glycerol immersion objective, at room temperature, and Zen Black 2.1 acquisition software (version 11.0.0.190, Carl Zeiss AG, Oberkochen, Germany). Image analysis was done using the Zen Blue Lite (version 2.0, Carl Zeiss AG, Oberkochen, Germany), PHOTO-PAINT, and CorelDraw programs from the CorelDRAW Graphics Suite 2017. Post-imaging processing was done using Corel PHOTO-PAINT 2017 (Corel Corporation, Ottawa, ON, Canada) using the brightness/contrast/intensity adjustment settings equally and simultaneously for images from the same imaging series.
6
Confocal Microscopy Analysis of Mitochondrial Fluorescence
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Images were obtained in the X, Y, and Z planes of the image, giving the image a 3-dimensional depth (Z-stack) at 100× magnification with a Zeiss Cell Observer Yokogawa Spinning Disk confocal microscope (Cell Observed SD, Carl-Zeiss, Oberkochen, Germany) located in the Microscopia Óptica de Luz Gustavo de Oliveira Castro (Plamol) platform in Universidade Federal do Rio de Janeiro. To measure the mean fluorescence intensity (MFI), Zen Lite Blue (Carl-Zeiss, Oberkochen, Germany) software was used. On average, thirty cells per field were analyzed in fourteen fields by experiments. Then, the region of interest (ROI), specifically the nucleus and mitochondria, was tagged on the cells photographed for fluorescence analysis, which is given by the software from the intensity of the pixels. Subsequently, the statistical test was performed using GraphPad Prism version 7.4, and the values presented are averages with the corresponding standard deviations (SDs).
7
MAVS Localization During EBV Lysis
8
MAVS Localization During EBV Lysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MAVS cDNA (DNASU#HsCD00296475) was cloned into pDEST-CMV-N-EGFP plasmid (a gift from Robin Ketteler, Addgene plasmid #122842110 (link) by Gateway cloning to produce pDEST-CMV-N-EGFP-MAVS plasmid. To visualize the mitochondria, the mito-BFP plasmid (a gift from Gia Voeltz, Addgene plasmid #49151111 (link) was used. Mito-BFP expresses BFP fused to an N-terminal mitochondrial targeting signal sequence obtained from COX4 amino acids 1–21. AGSiZ cells were plated in glass bottom 35 mm dish (ibidi #81158) at 0.4 million/ml. The next day, the plated cells were replenished with fresh medium at least 1h prior to co-transfection by pDEST-CMV-N-EGFP-MAVS and mito-BFP plasmids using Lipofectamine 2000 (Thermo) according to manufacturer’s instruction. Transfection medium was replaced by fresh medium after 6h and cells were incubated for an addition of 18h before live cell imaging. EBV lytic cycle was induced with 10 μg/ml Doxycycline and active lysosome was stained by 200 nM SiR-lysosome (#CY-SC012, Cytoskeleton, Inc.) during the course of experiment. At 200 mins post-lytic induction, image acquisition was performed with Zeiss LSM 800 instrument at an interval of 10 s for 15 mins and Zeiss Zen Lite (Blue) software was used for image analysis.
9
Simultaneous Calcium Imaging and Thermal Tolerance in Swimming Fish
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In experiment 3, calcium imaging in freely swimming fish was used to record CTmax and brain-wide depolarization simultaneously in the same individuals. The double-walled glass heating-mantle was placed under an epifluorescence microscope (AxioImager, ZEISS) equipped with a megapixel camera (AxioCam 506, ZEISS) and Plan-NEOFLUAR 2 ×1 objective. The larva was swimming at the center of the arena, within a 13-mm diameter region delimited with a nylon mesh, to match the limited field of view of the microscope (14 × 14 mm). Time lapse of fluorescent images was recorded at 5 Hz using the Zen software (ZEN Lite Blue, ZEISS). Since the recordings were made in the dark, the experimenter watched the live recording display of the recording on the computer screen to visually guide the pokes to the fish trunk. The nylon mesh created a small thermal gradient in the arena from the central part to the outer area outside the mesh. During the experimental trials, the temperature was recorded outside the mesh to ensure a full view of the fish. The temperature gradient was quantified in an additional experiment over six heat ramps by recording the temperature in the arena center and outside the mesh near the outer wall of the arena with two thermocouples at each location. The gradient was accounted for to calculate the water temperature the fish was exposed to.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!