Peripheral blood samples were collected into ethylene diamine tetra acetic acid (EDTA)-coated tubes from the facial vein, and plasma was separated by centrifugation. The quantification of HIV-1 RNA in the plasma of the virus-infected humanized NSG (hNSG) mice collected at 4 weeks post-infection was performed using the Taqman Analyzer according to the manufacturer’s instructions (Roche Diagnostics, Indianapolis, USA).
Taqman analyzer
Manufactured by Roche
Sourced in United States
The Taqman Analyzer is a real-time PCR (Polymerase Chain Reaction) instrument designed for high-throughput nucleic acid analysis. It utilizes fluorescence-based detection technology to quantify and analyze DNA or RNA samples. The core function of the Taqman Analyzer is to perform accurate and sensitive real-time PCR amplification and detection.
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Lab products found in correlation
2 protocols using taqman analyzer
1
Quantifying HIV-1 RNA in Humanized Mice
2
Quantifying Viral Load in Humanized Mice
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Peripheral blood samples were collected into ethylenediaminetetraacetic acid (EDTA)-coated tubes from the facial vein and plasma was separated from blood cells by low speed centrifugation. The quantification of PVL of the virus-infected humanized NSG mice collected at 6 and 10 weeks post-infection was performed using the Taqman Analyzer according to the manufacturer’s instructions (Roche Diagnostics, Indianapolis, IN, USA).
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