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Catalyst dx chemistry analyzer

Manufactured by IDEXX
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Sourced in United States, Montenegro

The Catalyst Dx Chemistry Analyzer is a diagnostic instrument designed for in-vitro analysis of various chemical compounds in animal samples. It provides automated processing and measurement of clinical chemistry parameters. The Catalyst Dx is intended to aid veterinary healthcare professionals in assessing the health status of animals.

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Lab products found in correlation

Procyte dx hematology analyzer, by IDEXX (10 mentions) Mbs723281, by MyBioSource (3 mentions) Caspase 3 assay kit, by Abcam (2 mentions) Myeloperoxidase activity assay kit, by Abcam (2 mentions) L lactate assay kit, by Abcam (2 mentions) St51011, by Shino-Test (2 mentions) Adi 900 212, by Enzo Life Sciences (2 mentions) Ca 7000, by Sysmex (2 mentions) Nsaid 6 clips, by IDEXX (2 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions) Il 18, by R&D Systems (1 mentions) Metabolic cage, by Harvard Apparatus (1 mentions) Ab65390, by Abcam (1 mentions) Meso quickplex sq 120, by Mesoscale (1 mentions) Vacutainer serum separation tube, by BD (1 mentions) Msd 5 plex proinflammatory panel 2 rat kit, by Mesoscale (1 mentions) Ultra sensitive rat insulin elisa kit, by Crystal Chem (1 mentions) Triple quad 5500 lc ms ms system, by AB Sciex (1 mentions) Il 6 elisa kit, by BioLegend (1 mentions) Mlb00c, by R&D Systems (1 mentions)

Close spelling variants of this product

Catalyst Dx Chemistry Analyzer system IDEXX Catalyst DX analyzer Catalyst® Chemistry Analyzer Dx Chemistry Analyzer Catalyst Dx

51 protocols using catalyst dx chemistry analyzer

1

Comprehensive Clinicopathological Evaluation in Veterinary Research

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The clinicopathological parameters statistically evaluated are listed in the supplementary Table S1. The CBC was performed using a laser haematology analyzer (IDEXX ProCyteDx® Hematology Analyzer, Idexx Laboratories, Westbrook, ME, USA). Blood smears were stained with May-Grünwald-Giemsa stain and examined to assess the morphology of blood cells, platelet estimate, leukocyte differential count and the detection of haemoparasites [68 (link)].
The biochemical profile was performed by the Catalyst Dx® Chemistry Analyzer (Idexx Laboratories, Westbrook, ME, USA), liquid chromatography-mass spectrometry for the evaluation of symmetric dimethylarginime (SDMA) (IDEXX Laboratories, Novara, Italia S.r.l) and by a latex agglutination reaction on an automated analyzer AU480 for the evaluation of serum amyloid A (SAA) (Beckman Coulter, Brea, California at the Department of Veterinary Medicine, Cambridge University, UK).
Urinalysis was performed by dipstick analysis (Combur 9 Test strips, Roche Diagnostics, Indianapolis, Indiana, USA), urine specific gravity (USG) was measured by a Vet 360 refractometer (Reichert, Seefeld, Germany) and microscopic evaluation of urine sediment by using the Kova glasstic slides (Kova International, Garden Grove, CA, USA). The UPC was assessed with the Catalyst Dx® Chemistry Analyzer (Idexx Laboratories, Westbrook, ME, USA).
Donato G., Masucci M., Hartmann K., Goris M.G., Ahmed A.A., Archer J., Alibrandi A, & Pennisi M.G. (2022). Leptospira spp. Prevalence in Cats from Southern Italy with Evaluation of Risk Factors for Exposure and Clinical Findings in Infected Cats. Pathogens, 11(10), 1129.
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2

Measurement of Biomarkers in Samples

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Commercially available enzyme-linked immunosorbent assay kits were used to measure the concentrations or activity of HMGB1 (no. ST51011, Shino-Test Corporation), IL-1β (no. MLB00C or no. DLB50, R&D Systems), IL-18 (no. 7625, R&D Systems), LDH (no. ab102526, Abcam), IL-6 (no. M6000B, R&D Systems), TNF (no. MTA00B, R&D Systems), cAMP (no. ADI-901-067, Enzo Life Sciences), and PKA (no. ab139435, Abcam) in indicated samples. Measurement of serum tissue enzymes (CK, BUN, and ALT) was performed using the IDEXX Catalyst Dx Chemistry Analyzer.
Chen R., Zeng L., Zhu S., Liu J., Zeh H.J., Kroemer G., Wang H., Billiar T.R., Jiang J., Tang D, & Kang R. (2019). cAMP metabolism controls caspase-11 inflammasome activation and pyroptosis in sepsis. Science Advances, 5(5), eaav5562.
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3

Metabolic, Enzymatic, and Apoptotic Assays

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Lactate in culture medium or serum was measured with the L-Lactate Assay Kit (Abcam; no. ab65331). Activation of caspase-3 in cell lysates was measured with the caspase-3 Assay Kit (Abcam; no. ab39401). Neutrophil activity was measured with the Myeloperoxidase Activity Assay Kit (Abcam; no. ab105136). Activation of tissue enzymes (CK, BUN, and ALT) in serum was performed using the IDEXX Catalyst Dx Chemistry Analyzer (IDEXX Laboratories).
Deng W., Zhu S., Zeng L., Liu J., Kang R., Yang M., Cao L., Wang H., Billiar T.R., Jiang J., Xie M, & Tang D. (2018). The Circadian Clock Controls Immune Checkpoint Pathway in Sepsis. Cell reports, 24(2), 366-378.
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4

Metabolic, Enzymatic, and Apoptotic Assays

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Lactate in culture medium or serum was measured with the L-Lactate Assay Kit (Abcam; no. ab65331). Activation of caspase-3 in cell lysates was measured with the caspase-3 Assay Kit (Abcam; no. ab39401). Neutrophil activity was measured with the Myeloperoxidase Activity Assay Kit (Abcam; no. ab105136). Activation of tissue enzymes (CK, BUN, and ALT) in serum was performed using the IDEXX Catalyst Dx Chemistry Analyzer (IDEXX Laboratories).
Deng W., Zhu S., Zeng L., Liu J., Kang R., Yang M., Cao L., Wang H., Billiar T.R., Jiang J., Xie M, & Tang D. (2018). The Circadian Clock Controls Immune Checkpoint Pathway in Sepsis. Cell reports, 24(2), 366-378.
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5

Kidney Function Assessment in Nephrectomized Rats

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To assess in vivo kidney function, native kidneys were removed from 10 rats with a human kidney and 10 control rats; animals were subsequently observed every 12 h until death. Nephrectomized rats were kept in metabolic cages (72-6970; Harvard Apparatus, Holliston, MA) to measure urine volume and to collect urine and blood samples. Blood samples were taken from the rat's tail vein. Renal function was assessed by measuring protein, creatinine and sodium in serum and urine samples using IDEXX Catalyst Dx Chemistry Analyzer (IDEXX Laboratories, Fremont, CA). A third group of 10 nonnephrectomized rats functioned as metabolic controls. The fractional excretion of sodium (FE Naþ ) was calculated from sodium and creatinine levels by the following equation:
Chang N.K., Gu J., Gu S., Osorio R.W., Concepcion W, & Gu E. (2015). Arterial flow regulator enables transplantation and growth of human fetal kidneys in rats. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 15(6).
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6

Mouse Serum ALT and AST Analysis

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Serum ALT and AST levels were determined in blood drawn from the mouse retro-orbital plexus using a Catalyst Dx Chemistry Analyzer (IDEXX Laboratories, Westbrook, ME, USA) according to the manufacturer’s instructions.
Cho Y.E., Kim Y., Kim S.J., Lee H, & Hwang S. (2023). Overexpression of Interleukin-8 Promotes the Progression of Fatty Liver to Nonalcoholic Steatohepatitis in Mice. International Journal of Molecular Sciences, 24(20), 15489.
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7

Evaluating SAA Levels in Foal Blood

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An 8.5 μL aliquot of whole anticoagulated (EDTA) blood from all foals was tested for SAA concentration using a commercially available stall‐side lateral flow immunoassay (Stablelab EQ‐1 Handheld Reader Zoetis, Parsippany‐Troy Hills, New Jersey). Thirty aliquots of the R. equi plasma and 6 of HiGamm, a hyperimmune plasma transfused to the foals also were tested for SAA. The precision and accuracy of the stall‐side assay is 98.6% and 95.6%, respectively, at concentrations ranging between 50 and 2000 mg/L.21 The handheld reader accurately detects SAA between 0 and 3000 μg/mL.21The CBC and SBP were performed using ProCyte Dx Hematology Analyzer (IDEXX, Westbrook, Maine), and Catalyst Dx Chemistry Analyzer (IDEXX, Westbrook, Maine), respectively. Serum IgG concentrations were measured using a DVM Rapid Test II—multitest analyzer (MAI Animal Health, Elmwood, Wisconsin) or Gamma Check E (PLASVACC, Templeton, California).
Palmisano M., Javsicas L., McNaughten J., Gamsjäger L., Renaud D.L, & Gomez D.E. (2023). Effect of plasma transfusion on serum amyloid A concentration in healthy neonatal foals and foals with failure of transfer of passive immunity. Journal of Veterinary Internal Medicine, 37(2), 697-702.
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8

Serum ALT Measurement Using Catalyst Dx® Analyzer

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Serum levels of ALT were measured using Catalyst Dx® Chemistry Analyzer (IDEXX Laboratories, Inc., Westbrook, ME) according to the manufacturer’s recommendations.
An P., Wei L.L., Zhao S., Sverdlov D.Y., Vaid K.A., Miyamoto M., Kuramitsu K., Lai M, & Popov Y.V. (2020). Hepatocyte mitochondria-derived danger signals directly activate hepatic stellate cells and drive progression of liver fibrosis. Nature Communications, 11, 2362.
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9

Comprehensive Analysis of Metabolic and Inflammatory Markers in Rodent Models

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Animals were euthanized using an overdose of sodium pentobarbital (200–300 mg/kg, Vortech; Dearborn, MI) administered by intraperitoneal injection, followed by exsanguination. Weight, nose-to-tail length, and abdominal girth were measured, and body mass index (BMI) was calculated. Blood was collected from the vena cava in EDTA-treated collection tubes and cells were analyzed using a ProCyte Dx Hematology Analyzer (IDEXX Laboratories, Inc.; Norcross, GA). Blood used for serum samples was collected in BD Vacutainer™ Serum Separation Tubes, left at room temperature for 1.5 h, and centrifuged at 25,000 g for 20 min. Fresh serum was used for measurements of high-density lipoprotein (HDL) (#ab65390, Abcam, Cambridge, MA) and blood chemistry (Catalyst Dx Chemistry Analyzer, IDEXX Laboratories, Inc). Epididymal fat pads were removed and weighed. Serum samples were stored at -80 °C until used for ELISA and cytokine analysis. ELISAs were conducted following the manufacturers’ protocols: leptin (#MOB00B, R&D Systems, Minneapolis, MN) and adiponectin (#Acrp30, R&D Systems). Insulin was measured using the Ultra-Sensitive Rat Insulin Elisa Kit (#90060, Crystal Chem; Elk Grove Village, IL). Serum cytokines were measured using the MSD V-PLEX Proinflammatory Panel 2 (rat) kit and MESO QuickPlex SQ 120 (Meso Scale Diagnostics; Rockville, MD).
Thompson J.A., Krajnak K., Johnston R.A., Kashon M.L., McKinney W, & Fedan J.S. (2021). High-fat western diet-consumption alters crystalline silica-induced serum adipokines, inflammatory cytokines and arterial blood flow in the F344 rat. Toxicology Reports, 9, 12-21.
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10

Hepatic Lipid and Enzyme Profiling

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Hepatic triglyceride and cholesterol levels were measured by the Infinity triglycerides and total cholesterol reagent kit (Thermo Fisher). Serum alanine aminotransferase (ALT) level and aspartate aminotransferase (AST) level were measured by Catalyst Dx Chemistry Analyzer (IDEXX) in the Boston University Analytical Instrumentation Core.
Tsukahara Y., Ferran B., Minetti E.T., Chong B.S., Gower A.C., Bachschmid M.M, & Matsui R. (2022). Administration of Glutaredoxin-1 Attenuates Liver Fibrosis Caused by Aging and Non-Alcoholic Steatohepatitis. Antioxidants, 11(5), 867.
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