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14c triolein

Manufactured by PerkinElmer
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14C-triolein is a radioactive tracer compound used in research applications. It consists of the triglyceride triolein, with one of the carbon atoms in the oleic acid chains labeled with the radioactive isotope carbon-14 (14C). This compound can be used to study lipid metabolism and transport processes in biological systems.

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Lab products found in correlation

Tyloxapol, by Merck Group (1 mentions) Tricarb scintillation counter, by PerkinElmer (1 mentions) Infinity triglyceride, by Thermo Fisher Scientific (1 mentions) Autokit glucose, by Fujifilm (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Hr series nefa hr 2, by Fujifilm (1 mentions) Qpcr core kit for sybr green 1, by Eurogentec (1 mentions) Infinity cholesterol, by Thermo Fisher Scientific (1 mentions) Phospholipids c, by Fujifilm (1 mentions) 14c oleic acid, by PerkinElmer (1 mentions) 3h cholesterol, by PerkinElmer (1 mentions) Omniscript rt, by Qiagen (1 mentions) Poloxamer 407, by Spectrum Chemical (1 mentions)

Spelling variants of this product

Triolein (9 citations) [1-14C]triolein (5 citations)

5 protocols using 14c triolein

1

Chylomicron Production Kinetics in Mice

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To investigate the rate of chylomicron production in the intestine, mice were injected intravenously with 0.5 mg/g BW Triton WR-1339 (Tyloxapol (Sigma); 10% solution in PBS) before receiving an oral gavage of olive oil containing 14C-Triolein (Perkin-Elmer; 1.5 kBq 14C-Triolein/g BW in 2.6 µL olive oil/g BW). Blood samples were taken from the tail vein before gavage and 30, 60, 120, and 240 min after gavage. Plasma samples were mixed with scintillation fluid and radioactivity was measured using a Perkin Elmer Tricarb scintillation counter and expressed as cpm per µL plasma.
Pauter A.M., Fischer A.W., Bengtsson T., Asadi A., Talamonti E, & Jacobsson A. (2019). Synergistic Effects of DHA and Sucrose on Body Weight Gain in PUFA-Deficient Elovl2 -/- Mice. Nutrients, 11(4), 852.
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2

Comprehensive Lipid Metabolism Profiling

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Infinity Cholesterol (catalog #TR13421), Infinity Triglyceride (catalog #TR22421), and TRIzolTM (catalog #15596018) reagents were purchased from Thermo Fisher Scientific (Middletown, VA, USA). Autokit Glucose (catalog #997-03001), Phospholipids C (catalog #997-01801), and HR Series NEFA-HR(2) (catalog #999-34691, 995-34791, 991-34891, and 993-35191) kits were purchased from Fujifilm Wako Chemicals USA (Richmond, VA, USA). Omniscript RT (catalog #205113) kit was purchased from Qiagen (Germantown, MD, USA) and qPCRTM core kit for SYBR Green I (catalog #10-SN10-05) was from Eurogentec (San Diego, CA, USA). 3H-cholesterol (catalog #NET139001MC), 14C-oleic acid (catalog #NEC317250UC), and 14C-triolein (catalog #NEC674250UC) were from PerkinElmer (Shelton, CT, USA). Poloxamer 407 (catalog #P1166) was purchased from Spectrum Chemical (New Brunswick, NJ, USA). Primary and secondary antibodies were purchased from either Cell Signaling (Danvers, MA, USA) or Abcam (Cambridge, MA, USA). All other chemicals and solvents were obtained from Fisher Scientific through its local distributor in the Kingdom of Saudi Arabia.
Otaibi A.A., Mubarak S.A., Qarni A.A., Hawwari A., Bakillah A, & Iqbal J. (2022). ATP-Binding Cassette Protein ABCC10 Deficiency Prevents Diet-Induced Obesity but Not Atherosclerosis in Mice. International Journal of Molecular Sciences, 23(22), 13813.
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3

Triglyceride Hydrolase Enzyme Assay

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Protein was extracted from primary enterocytes in 100 mM potassium phosphate buffer by brief sonication. For Figure 5A, primary enterocytes were incubated with rMFGE8 or RGE proteins (10 μg/mL) in serum-free media for 1 h before proceeding with protein isolation. 60–100 μg protein was incubated with 100 μL TG substrate (25 nmol triolein/assay and 40,000 cpm/nmol 14C-triolein; PerkinElmer) and 35.5 μg mixed micelles of phosphatidylcholine and phosphatidylinositol (3:1, w/w), respectively, for 1 h at 37. After 1 h, the reaction was terminated by adding 3.25 mL methanol/chloroform/heptane (10:9:7, v/v/v) and 1 mL 100 mM potassium carbonate (pH 10.5 with boric acid). After centrifugation (800×g, 15 min, 4°C), radioactivity was measured in 1 mL of the upper phase by liquid scintillation counting.11 (link) The radioactivity counts were normalized relative to protein concentration and the TG hydrolase activity was expressed as relative fold changes to the untreated control samples.
Itoh A, & Maki T. (1996). Protection of nonobese diabetic mice from autoimmune diabetes by reduction of islet mass before insulitis.. Proceedings of the National Academy of Sciences of the United States of America, 93(20), 11053-11056.
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4

Triglyceride Hydrolase Enzyme Assay

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Protein was extracted from primary enterocytes in 100 mM potassium phosphate buffer by brief sonication. For Figure 5A, primary enterocytes were incubated with rMFGE8 or RGE proteins (10 μg/mL) in serum-free media for 1 h before proceeding with protein isolation. 60–100 μg protein was incubated with 100 μL TG substrate (25 nmol triolein/assay and 40,000 cpm/nmol 14C-triolein; PerkinElmer) and 35.5 μg mixed micelles of phosphatidylcholine and phosphatidylinositol (3:1, w/w), respectively, for 1 h at 37. After 1 h, the reaction was terminated by adding 3.25 mL methanol/chloroform/heptane (10:9:7, v/v/v) and 1 mL 100 mM potassium carbonate (pH 10.5 with boric acid). After centrifugation (800×g, 15 min, 4°C), radioactivity was measured in 1 mL of the upper phase by liquid scintillation counting.11 (link) The radioactivity counts were normalized relative to protein concentration and the TG hydrolase activity was expressed as relative fold changes to the untreated control samples.
Datta R., Gholampour M.A., Yang C.D., Volk R., Lin S., Podolsky M.J., Arnold T., Rieder F., Zaro B.W., Verzi M., Lehner R., Abumrad N., Lizama C.O, & Atabai K. (2023). MFGE8 links absorption of dietary fatty acids with catabolism of enterocyte lipid stores through HNF4γ-dependent transcription of CES enzymes. Cell reports, 42(3), 112249.
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5

Radiolabeled VLDL Clearance Kinetics

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VLDL was isolated from human plasma by density gradient ultracentrifugation.14Ctriolein (Perkin Elmer, MA) was dried down in a glass tube under a stream of N2 to obtain a dry lipid film. Isolated VLDL was added to the dried lipid film and sonicated on ice for 15–20 minutes. Approximately, 20 μCi of[14C]triolein/ml of VLDL was used for 20 mice. VLDL was then dialyzed in a buffer containing 150mM NaCl and 0.24mM EDTA at 4C and counts were measured. Radiolabeled VLDL was then diluted with PBS and injected intravenously in mice (1*106 cpm/mouse). Blood samples were collected before (0) and 30 seconds, 5 minutes, 15 minutes, 30 minutes and 60 minutes after injection. Plasma was separated and counts were measured using 10 μl plasma in a scintillation counter.
Basu D., Huggins L.A., Scerbo D., Obunike J., Mullick A.E., Rothenberg P.L., Di Prospero N.A., Eckel R.H, & Goldberg I.J. (2018). Mechanism of increased LDL and decreased triglycerides with SGLT2 inhibition. Arteriosclerosis, thrombosis, and vascular biology, 38(9), 2207-2216.
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