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Advia 1800

Manufactured by Fujifilm
Sourced in Japan

The ADVIA 1800 is a clinical chemistry and immunoassay analyzer developed by Fujifilm. It is designed to perform a wide range of diagnostic tests, including biochemical and immunological analyses, to support clinical decision-making in medical settings.

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2 protocols using advia 1800

1

Metabolic Biomarker Assessment Protocol

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After at least eight hours of fast, blood samples were collected from the antecubital vein in the morning. Fasting blood glucose level was determined by a colorimetry method (ADVIA 1800, Siemens, USA). Insulin level was measured by a chemiluminescence immunoassay (Centaur XP, Siemens, USA). Homeostasis model assessment of insulin resistance (HOMA-IR) was used to evaluate insulin resistance: HOMA-IR = fasting plasma glucose (mg/dL) × fasting insulin (μIU/mL)/405[26 (link)]. An enzymatic method (ADVIA 1800, Siemens, USA) was used to assess total cholesterol, high-density lipoprotein (HDL) cholesterol and triglycerides. C-reactive protein (CRP) concentration was assessed by a turbidometric immunoassay (ADVIA 1800, Wako, Japan). Low-density lipoprotein (LDL) cholesterol was calculated according to the Friedewald formula: LDL cholesterol = Total cholesterol—HDL cholesterol—(triglycerides/5) (all concentrations in mg/dL)[27 (link)]. HbA1c levels were measured by high-performance liquid chromatography using the VARIANT II system (Bio-Rad, Hercules, CA, USA).
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2

Metabolic Profile Assessment Protocol

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After at least 8 h of fasting, blood samples were collected from the antecubital vein in the morning. Fasting blood glucose levels were determined by a colorimetry method (ADVIA 1800; Siemens Medical Solutions, Pleasanton, CA, USA), and fasting insulin levels were determined using an immunoradiometric assay (SR-300; Stratec, Birkenfeld, Germany). Enzymatic methods were used to measure the total cholesterol, high-density cholesterol (HDL) and triglyceride levels (ADVIA 1800; Siemens Medical Solutions, Pleasanton, CA, USA). Low-density lipoprotein cholesterol levels were calculated using the Friedewald formula. 11 C-reactive protein (CRP) concentration was assessed with a turbidimetric immunoassay (ADVIA 1800, Wako, Japan). The homeostasis model assessment of insulin resistance (HOMA-IR) was used to evaluate insulin resistance: HOMA-IR = fasting glucose (mg dl À 1 ) × fasting insulin (μIU ml À 1 )/405. 12 Incident diabetes was defined by a fasting glucose level ⩾ 126 mg dl À 1 or an HbA1c ⩾ 6.5% at the follow-up examination and/ or a physician's diagnosis of diabetes during the follow-up period.
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