After the assessment of the pancreatic blood flow, blood samples were taken from the abdominal aorta for biochemical analysis.
The prothrombin time, expressed as the international normalized ratio (INR), was checked in the fresh blood using Alere INRatio
® 2 PT/INR Monitoring Systems and Alere INRatio
® PT/INR Monitoring System Test Strips (Alere San Diego, Inc., San Diego, CA, USA).
The plasma D-dimer concentration was measured using an immunoturbidimetric assay (
Innovance D-Dimer Assay, Siemens Healthcare GmbH, Marburg, Germany) on the automatic coagulation analyzer
BCS XP System (Siemens Healthcare Diagnostics, Erlangen, Germany). The results are presented in µg/mL.
Serum lipase and amylase activity was determined with a
Kodak Ectachem DT II System analyzer (Eastman Kodak Company, Rochester, NY, USA) using Lipa and Amyl DT Slides (Vitros DT Chemistry System, Johnson & Johnson Clinical Diagnostic, Inc., Rochester, NY, USA). The results are presented in U/L.
The serum level of interleukin-1β (IL-1β) was measured using the Rat IL-1β Platinum Elisa (Bender MedSystem GmbH, Vienna, Austria). This kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat interleukin—1β. The results are presented in pg/mL.
Konarska-Bajda K., Ceranowicz P., Cieszkowski J., Ginter G., Stempniewicz A., Gałązka K., Kuśnierz-Cabala B., Dumnicka P., Bonior J, & Warzecha Z. (2023). Administration of Warfarin Inhibits the Development of Cerulein-Induced Edematous Acute Pancreatitis in Rats. Biomolecules, 13(6), 948.