Norharman

Manufactured by LGC

Sourced in Canada

Norharman is a chemical compound used in scientific research and laboratory applications. It functions as a standard reference material and an analytical tool for various analytical techniques. The specific details and intended uses of Norharman should be obtained from technical literature or the product manufacturer.

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Lab products found in correlation

Trp p 2, by LGC (3 mentions) Trp p 1, by LGC (3 mentions) Meaαc, by LGC (3 mentions) Harman, by LGC (2 mentions) Glu p 1, by LGC (2 mentions) Oasis mcx cartridge, by Waters Corporation (2 mentions) Creatinine, by Merck Group (1 mentions) Free amino acids, by Merck Group (1 mentions) Creatine, by Merck Group (1 mentions) Curcumin, by Yuanye Bio-Technology (1 mentions) Tryptophan, by Yuanye Bio-Technology (1 mentions) Phenylalanine, by Yuanye Bio-Technology (1 mentions) Creatine, by Yuanye Bio-Technology (1 mentions) Dichloromethane, by Sinopharm (1 mentions) Acetic acid, by Sinopharm (1 mentions) Sodium hydroxide (naoh), by Sinopharm (1 mentions) Ammonium acetate, by Sinopharm (1 mentions) Ammonium hydroxide, by Sinopharm (1 mentions) Diethylene glycol, by Sinopharm (1 mentions) Milli q, by Merck Group (1 mentions)

4 protocols using norharman

Determination of Heterocyclic Amines in TRE

Cited 1 time

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All of the chemicals and solvents were of HPLC or analytical grade. Analytical standards of 12 Has—IQ (2-amino-3-methyl-imidazo[4,5-f]quinoline), MeIQ (2-amino-3,4-dimethyl-imidazo[4,5-f]quinoline), MeIQx (2-amino-3,8-dimethyl-imidazo [4,5-f]quinoxaline), 4,8-DiMeIQx (2-amino-3,4,8-trimethyl-imidazo[4,5-f]quinoxaline), 7,8-DiMeIQx (2-amino-3,7,8-trimethyl-imidazo[4,5-f]quinoxaline), PhIP (2-amino-1- methyl-6-phenylimidazo[4,5-f]pyridine), Harman (1-methyl-9H-pyrido[3,4-b]indole), Norharman (9H-pyrido[3,4-b]indole), Trp-P-2 (3-amino-1-methyl-5H-pyrido[4,3-b] indole), Trp-P-1 (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole), AαC (2-amino-9H- pyrido[2,3-b]indole), and MeAαC (2-amino-3-methyl-9H-pyrido[2,3-b]indole)—were supplied by Toronto Research Chemicals (Downsview, ON, Canada). The standards of free amino acids, creatine, and creatinine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Oasis MCX cartridges (60 mg, 3 mL) were supplied by Waters (Milford, MA, USA). The TRE was obtained followed our previous described [16 (link)] with the extraction conditions of extraction solvent of 60% ethanol, ultrasonic-assisted extraction power of 600 W for 40 min. The vacuum freeze dryer was employed to obtain the extract powders, which were stored at −20 °C until use.

Ren X., Li M., Wang W., Niu X., Xu Q, & Zhang R. (2022). Inhibitory Effect of Tamarix ramosissima Extract on the Formation of Heterocyclic Amines in Roast Lamb Patties by Retarding the Consumption of Precursors and Preventing Free Radicals. Foods, 11(7), 1000.

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Quantification of Bioactive Compounds

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Chicken breast (recessive white chicken) and turmeric were purchased from the local supermarket (Wuhan, Hubei, China). Nor harman and harman were obtained from Toronto Research Chemicals Inc. (Downsview, Ont., Canada). Curcumin, tryptophan, phenylalanine, and creatine were purchased from Shanghai yuanye Bio‐Technology Co., Ltd (Shanghai, China). Methanol and acetonitrile (High Performance Liquid Chromatography (HPLC) grade) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Acetic acid, ammonium acetate, sodium hydroxide, ammonium hydroxide, dichloromethane, diethylene glycol, and hydrochloric acid glucose were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The MCX SPE column (Oasis® MCX 3cc, 60 mg, Extraction Cartridges) was obtained from Waters Corporation (Waters, Milford, MA, USA).

Wang Q., Li J., Li K, & Li C. (2021). Effects of turmeric on reducing heterocyclic aromatic amines in Chinese tradition braised meat products and the underlying mechanism. Food Science & Nutrition, 9(10), 5575-5582.

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Heterocyclic Aromatic Amines Analysis in Meat

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All chemicals and solvents were of HPLC or analytical grade. Acetonitrile, and Methanol were purchased from Merck (Germany). Formic acid, Hydrogen chloride (36.5–38%), Sodium chloride, and Magnesium sulfate was obtained from J.T.Baker (USA) was ACS grade. Ultrapure water (Milli-Q) was obtained from Millipore system (France). The heterocyclic aromatic amines (HCAs) were used as reference compounds: IQ, MeIQ, 8-MeIQx, PhIP, AαC, MeAαC, Harman, NorHarman, Trp-P-1, Trp-P-2, and Glu-P-1 were purchased from Toronto Research Chemicals (Canada). Standard stock solutions of 10 μg/g in Methanol were prepared and used for further dilution. 4,7,8-TriMeIQx was used as an internal standard (150 ng/g). The three types of sorbents used in QuEChERS are listed below: Primary and secondary amine (PSA) SPE bulk sorbent (Agilent Technologies, USA), SiliaBondR C18 (17%), 40–63 μm, 60 A (Silicycle^® Inc, Canada), C18 Endcapped (C18EC), 57.9 μm, 59 A (Agilent Technologies, USA). Oasis^® MCX Cartridge, 30 μm by Waters (USA). The analysis column was a Shim-pack GIST C18 (2.1 × 100 mm, 3 μm, 100 Å) from Shimadzu (Japan). Meat samples (pork floss, pork jerky, beef jerky, raw pork and raw beef) were purchased from local stores and stored in a freezer at −18 °C until used.

Jian S.H., Yeh P.J., Wang C.H., Chen H.C, & Chen S.F. (2018). Analysis of heterocyclic amines in meat products by liquid chromatography – Tandem mass spectrometry. Journal of Food and Drug Analysis, 27(2), 595-602.

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Quantification of Heterocyclic Amines

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Harman, Norharman, AαC, MeAαC, Trp-P-1, Trp-P-2, Glu-P-1, Glu-P-2, IQ, PhIP and their corresponding isotope-labeled analogs—Harman-¹³C2, ¹⁵N; Norharman-D7; AαC-¹⁵N3; MeAαC-D3; Trp-P-1-¹³C2,¹⁵N; Trp-P-2-¹³C2,¹⁵N; Glu-P-1-¹³C3; Glu-P-2-¹³C2,¹⁵N; IQ-D3; and PhIP-D3—were purchased from Toronto Research Chemicals Inc. (North York, ON, Canada). LC/MS grade acetonitrile (ACN) from Fisher Scientific (Pittsburgh, PA, USA) and LC/MS grade water from J.T. Baker (Center Valley, PA, USA) were used for mobile phase preparation. LC/MS grade ammonium hydroxide from Sigma-Aldrich (St. Louis, MO, USA) was used for pH adjustment. All other reagents were at least high performance liquid chromatography (HPLC) grade. Ninety-six-well plates of ISOLUTE SLE+ (supported liquid extraction) and EVOLUTE® CX (mix mode of cation exchange resin, CX) for sample preparation were purchased from Biotage Inc (Charlotte, NC, USA).

Zhang L., Xia Y., Xia B., Nicodemus K.J., McGuffey J., McGahee E., Blount B, & Wang L. (2016). High throughput and sensitive analysis of urinary heterocyclic aromatic amines using isotope-dilution liquid chromatography-tandem mass spectrometry and robotic sample preparation system. Analytical and bioanalytical chemistry, 408(28), 8149-8161.

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