Single HER2+ or HER2− CTCs were flow sorted in 96-well white-walled plates (Corning) using Laser BD FACS Aria Fusion Cell Sorter, BSL2+. Single cell, 1, 3, 5–9, 10–20 and <20-cell clones were analyzed for heterogeneity in HER2 expression via staining with antibodies against EpCAM (FITC labeled; Cell Signaling, clone VU1D9) and HER2 (APC labeled, BD, clone 42 c-erbB-2). Imaging and image processing was performed sequentially with the confocal microscope (Zeiss 710 Laser Scanning Confocal) followed by FIJI (Image J). Images are representative of at least 20 independent images per colony size.
Laser facs aria fusion cell sorter
Manufactured by BD
The Laser BD FACS Aria Fusion Cell Sorter is a high-performance flow cytometry instrument designed for cell sorting applications. It features multiple laser configurations and customizable fluidics systems to enable advanced cellular analysis and sorting capabilities.
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Lab products found in correlation
2 protocols using laser facs aria fusion cell sorter
1
Single cell HER2 heterogeneity analysis
2
Single cell HER2 heterogeneity analysis
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