Raman maps were acquired using a Renishaw in Via Raman microscope. The excitation laser was either a 632.8 nm HeNe laser (ThorLabs) for the n-mercaptobutylnitrile and p-mercaptobenzonitrile monolayer experiments or a 660 nm diode laser (Laser Quantum) for the CN− adsorbed to Ag experiments. The acquisition time per spectrum was adjusted with the incident laser power to generate spectra with sufficient signal to noise ratios for analysis. For CN experiments, the scattering between 1940–2348 cm−1 Raman shift was collected; otherwise, the spectral range was 2016–2470 cm−1. Raman spectra for gold nanoparticles on ultraflat gold films were taken using a homebuilt Raman microscope equipped with dark field microscopy (BD objective, Olympus, LMPlanFLN, NA=0.5). A 632.8 nm HeNe laser (Melles Griot) was used to irradiate the sample in a top illumination geometry and a Horiba Jobin Yvon iHR320 spectrometer was used to resolve the Raman scattering. The laser power measured at the sample was 0.75mW, and the acquisition time was 1s. The spectral data was analyzed using MATLAB and an open-source peak-fitting routine.42 Spectra were fit to a Gaussian lineshape 5 times, and fit of the lowest % RMS error was selected.
Lmplanfl n
Manufactured by Olympus
Sourced in Japan
The LMPlanFL N is a high-performance microscope objective lens designed for fluorescence microscopy applications. It features a numerical aperture of 0.5 and a working distance of 10.6 mm. The lens is optimized for use with common fluorescent dyes and provides high-quality, high-contrast images.
Automatically generated - may contain errors
Lab products found in correlation
Labram hr raman spectrometer, by Horiba (3 mentions)
Labram hr, by Horiba (3 mentions)
Ihr320 spectrometer, by Horiba (2 mentions)
632.8 nm hene laser, by Thorlabs (2 mentions)
Invia raman microscope, by Renishaw (2 mentions)
Monochromator, by Horiba (1 mentions)
Razoredge, by IDEX Corporation (1 mentions)
La1461, by Thorlabs (1 mentions)
Zyla 4, by Oxford Instruments (1 mentions)
A240, by Thorlabs (1 mentions)
Synapse, by Horiba (1 mentions)
Labram bx41, by Horiba (1 mentions)
Ihr320, by Horiba (1 mentions)
Labspec 6, by Horiba (1 mentions)
Quanta 200 3d, by Thermo Fisher Scientific (1 mentions)
Xcd sx90cr, by Sony (1 mentions)
Jsm 6700f, by JEOL (1 mentions)
Th4 200, by Olympus (1 mentions)
Uv vis nir spectrometer, by Agilent Technologies (1 mentions)
Cary 5000, by Agilent Technologies (1 mentions)
21 protocols using lmplanfl n
1
Raman Spectroscopy of Surface-Adsorbed Species
2
Combined AFM-Raman TERS Imaging Protocol
3
Raman Spectroscopic Analysis of ABS Nanocompounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyze the molecular bonds present in the pure ABS and the fabricated nanocompounds, measurements through Raman spectroscopy were accomplished. The Raman analysis was conducted utilizing a customized LabRAM HR Raman Spectrometer (HORIBA Scientific, Kyoto, Japan). The irritation source for Raman was obtained by employing a solid-state laser module with a 532 nm central wavelength and maximum laser output power of 90 mW. The microscope in the analysis utilized a 50× microscopic objective lens, which had a working distance of 10.6 mm and a numerical aperture of 0.5 (LMPlanFL N, Olympus, Tokyo, Japan). This objective lens was responsible to provide the excitation light on the sample and to gather the Raman signals. In parallel, a neutral density filter with a 5% transmittance was used, resulting in 2 mW of power on the sample. The resulting laser spot size was approximately 1.7 μm laterally and 2 μm axially. The Raman spectral resolution was around 2 cm−1 using the 600 grooves grating of the spectrometer. The purchased Raman spectral range was set between 50 to 3900 cm−1, providing three optical windows per point. Each measurement took a total of 50 s for acquisition when five accumulations were conducted at each point.
4
Thermal and Structural Analysis of 3D-Printed Nanocomposites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thermogravimetric analysis (TGA) measurements (Perkin Elmer Diamond, Perkin Elmer Inc., Waltham, MA, USA, 30–550 °C, step 10 °C/min, a Nitrogen atmosphere) were taken on samples of approximately 10 mg, taken from the 3D printed samples, to determine the thermal properties and stability of the produced nanocomposites.
Raman measurements were performed with a modified LabRAM HR Raman Spectrometer (HORIBA Scientific, Kyoto, Japan). Raman excitation was achieved with a 532 nm central wavelength solid-state laser module with a maximum laser output power of 90 mW. The microscope is coupled with a 50× microscopic objective lens with a 0.5 numerical aperture and 10.6 mm working distance (LMPlanFL N, Olympus) that delivered the excitation light and collected the Raman signals. A neutral density filter of 5% transmittance was used, which resulted in 2 mW of power on the sample. The laser spot size was approximately 1.7 μm laterally and approximately 2 μm axially. A 600 grove grating was used, resulting in a Raman spectral resolution of approximately 2 cm−1. The Raman spectral range was set to be from 500 to 3900 cm−1, resulting in 3 optical windows per point. The acquisition time for each measurement was 10 s and with five accumulations at each point.
Raman measurements were performed with a modified LabRAM HR Raman Spectrometer (HORIBA Scientific, Kyoto, Japan). Raman excitation was achieved with a 532 nm central wavelength solid-state laser module with a maximum laser output power of 90 mW. The microscope is coupled with a 50× microscopic objective lens with a 0.5 numerical aperture and 10.6 mm working distance (LMPlanFL N, Olympus) that delivered the excitation light and collected the Raman signals. A neutral density filter of 5% transmittance was used, which resulted in 2 mW of power on the sample. The laser spot size was approximately 1.7 μm laterally and approximately 2 μm axially. A 600 grove grating was used, resulting in a Raman spectral resolution of approximately 2 cm−1. The Raman spectral range was set to be from 500 to 3900 cm−1, resulting in 3 optical windows per point. The acquisition time for each measurement was 10 s and with five accumulations at each point.
5
Automated Nonlinear Microscopy for SHG Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SHG intensity spectrum was measured with a fully-automated homemade nonlinear microscope system as illustrated in Fig. 4c . A pulsed light with tunable wavelength was generated by a Ti:Sapphire laser system (Chameleon Ultra II, Coherent) combined with an optical parametric oscillator (Compact OPO, Coherent). The pulses wavelength was tuned from 1050 nm to 1350 nm with a duration typically around 200 fs, a repetition rate of 80 MHz and an average power at the sample of 5 mW. The beam was focused to a beam radius of 5 μm on the sample with a lens (A240TM with NA = 0.5, Thorlabs) and collected with a 100× objective (LMPlanFL N with NA = 0.8, Olympus). The sample was firmly attached on both sides and stretching was achieved by translating the two sample holders in opposite directions (precision of translation is 0.1 mm for a 15 mm rest length). The signal was focused with a convex lens (la1461 with f = 250 mm, Thorlabs) onto a sCMOS camera (Zyla 4.2, Andor) and the SH signal was separated using two high-pass filters.
6
SERS Analysis of Biotin-Streptavidin Interaction
7
Confocal Microscopy of NV Emission
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Our confocal microscope set-up is equipped with a diode-pumped and frequency-doubled Nd:YAG-laser operating at a wavelength of 532 nm with an output power of 5 mW and a commercial confocal microscope (LabRam BX41, Horiba Jobin Yvon). The laser beam is passed to the microscope through an optical fibre and focused onto the sample by the microscope objective. The laser power illuminating the sample is 1 mW due to losses in the optical path of the microscope setup. The resulting photoluminescence signal is collected by the same objective and the excitation wavelength is spectrally filtered out using notch filter. The light beam is subsequently focused onto a pinhole for spatial filtering and routed via a fibre to a monochromator (iHR-320, Horiba Jobin Yvon). As detector a Peltier-cooled CCD-Camera (SYNAPSE, Horiba Jobin Yvon) was used.
To investigate the tuned NV emission, we used an objective with a long working distance (Olympus LMPlanFLN, 50×, NA = 0.5, WD = 10.6 mm) to avoid a short circuit between the microscope objective and the metallized and wired diamond surface.
To investigate the tuned NV emission, we used an objective with a long working distance (Olympus LMPlanFLN, 50×, NA = 0.5, WD = 10.6 mm) to avoid a short circuit between the microscope objective and the metallized and wired diamond surface.
8
Raman Spectroscopy Protocol for Material Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A specially designed Raman spectrometer LabRAM HR (from HORIBA Scientific, Kyoto, Japan), was used to conduct the Raman analysis. A module for a solid-state laser with a supreme output power of 90 mW and a central operating wavelength of 532 nm was used as the excitation source for Raman spectroscopy. The samples were illuminated with excitation light, and Raman signals were collected using a microscope (LMPlanFL N, Olympus, Tokyo, Japan) with a 10.6 mm operating distance, a 0.5 numerical leak, and a 50 microscopic objective lens. To lower the power on the specimen to 2 mW, a 5% transmittance neutral density filter was also employed. The laser point size, as a result, was roughly 2 μm axially and 1.7 μm laterally. Each measurement took 50 s to acquire, with five accumulations being conducted at each point. Three optical windows were provided by the acquired Raman spectrum ranging from 50 to 3900 cm−1, and the resolution of the Raman spectrum was about 2 cm−1. The spectrometer’s 600-groove grating was used.
9
Raman, PL, and AFM Analysis of Nanomaterials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All Raman and PL measurements were performed by using a LabRam HR (Horiba, Edison, NJ, USA) with a 100× objective (LM Plan FL N, NA = 0.80, Olympus, Center Valley, PA, USA). The excitation source was a 532.06 nm laser (Laser Quantum, Fremont, CA, USA). Detection was performed by using a one-electrode thermoelectrically cooled CCD detector (Andor Technology Inc., Belfast, Northern Ireland). The entire system was controlled by LabSpec 6 software (Horiba).
Atomic force microscopy (AFM) measurements were recorded by using an AIST-NT model SmartSPM 1000 in intermittent contact mode with an Al-coated Si probe (k = 2 N/m, 240 µm length) (AC240TM-R3, Oxford Instruments Asylum Research, Santa Barbara, CA, USA) operating at a 70 kHz resonant frequency and controlled by Omega software 3.5.81 (AIST-NT).
Atomic force microscopy (AFM) measurements were recorded by using an AIST-NT model SmartSPM 1000 in intermittent contact mode with an Al-coated Si probe (k = 2 N/m, 240 µm length) (AC240TM-R3, Oxford Instruments Asylum Research, Santa Barbara, CA, USA) operating at a 70 kHz resonant frequency and controlled by Omega software 3.5.81 (AIST-NT).
10
Characterizing Fluorescent Nanodiamonds via Confocal Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyze the fluorescence and optically detected magnetic resonance (ODMR) spectra of the fluorescence nanodiamonds (FNDs), we designed and built a confocal laser scanning microscope. This confocal microscope was equipped with a high magnification microscope objective (100×, NA = 0.8, model number: LMPlanFL N, Olympus, Tokyo, Japan), multi-color lasers, and an integrated microwave system. The FND samples were attached to a microwave board and placed on the confocal setup. FND samples were then scanned in the x–y directions by a green (532 nm) laser (max power = 150 mW) using Thorlabs GVS 212 Galvano scanners (Newton, NJ, USA). The fluorescence spectra were collected through the same microscope objective and analyzed with a custom-made spectrometer equipped with a starlight camera (Trius camera model SX-674, Starlight Xpress Ltd., Bottle Lane, UK) and a photon counter (Hamamatsu photon counter model number H7155-21, Hamamatsu Photonics UK Limited, Welwyn Garden City, UK). For the ODMR, the microwave (MW) frequencies were swept over a specific range (for example, from 2700 MHz to 3000 MHz), and the fluorescent counts were plotted vs. MW frequency.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!