Structural MRI images were used to define the model for the volume conductor and the solution space for MEG source localization analysis. Images were acquired with a General Electric 1.5 Tesla using an eight-channel head coil. The imaging protocol consisted of: 3D T1-weighted high-resolution images using a Fast-Spoiled Gradient Echo sequence [TR/TE/TI=11.2/4.2/450 ms; flip-angle= 12°; FoV= 250 mm; acquisition matrix= 256 × 256; slice thickness=1 mm]. Structural images were used to reconstruct each person´s cortical surface with FreeSurfer software. Inner skull surface was used for a boundary element model of the volume conductor in the forward calculations. The solution space was approximated by ∼5000 free-rotation dipoles along the gray-white matter surface in the cortex, with spacing between dipole locations ∼7 mm (Kovacevic et al., 2012 (link)).
1.5 tesla
Manufactured by GE Healthcare
Sourced in United States, United Kingdom
The 1.5 Tesla is a magnetic resonance imaging (MRI) system manufactured by GE Healthcare. It is designed to produce high-quality images of the human body. The 1.5 Tesla refers to the strength of the magnetic field used in the system, which is a common specification for MRI scanners.
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5 protocols using 1.5 tesla
1
MRI-based MEG Source Localization Protocol
2
Quantitative PiB PET Imaging Protocol
3
Stereotactic Bilateral Capsulotomy Procedure
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The capsulotomy procedure used in this study followed the protocol described by Loughman et al16 Specifically, a Leksell stereotactic frame (Elekta Inc, Stockholm, Sweden) was mounted on the patient's head under local anesthesia or mild sedation. After placement of the frame, the patient underwent a preoperative MRI scan at 1.5 Tesla (GE Healthcare, Madison, WI, USA). Visualizing the internal capsule on stereotactic MRIs enabled the identification of the target. The target was 15‐17 mm anterior to the anterior commissure (AC), 15‐17 mm lateral to the midline, and 2‐4 mm under the AC‐posterior commissure (PC) line. After calculating the stereotactic target and designing the trajectory, bilateral burr holes were made anterior to the coronal suture based on the measured entrance trajectory. The procedure was performed under general anesthesia after dural opening and cauterization of the pia‐arachnoid. A 2‐mm (diameter) and 3‐mm uninsulated tip radiofrequency electrode (Radionics, Burlington, MA, USA) was used for impedance measurement, followed by a stimulation test and lesion. The radiofrequency lesions were made at 80°C for 60 seconds. The first lesion was located 3‐4 mm below the AC‐PC line, and the total lesioned length was 14 mm bilaterally. A representative MRI performed after this procedure is shown in Figure 1 .
4
Cardiac Magnetic Resonance Imaging Protocol
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Formalin‐fixed hearts were analyzed by cardiac magnetic resonance imaging at 1.5 Tesla (GE Healthcare, Belford, UK) using a T2W pulse sequence. Remodeling parameters (maximum and minimum infarcted wall thickness [Tmax and Tmin infarcted wall, respectively]; noninfarcted wall thickness was measured just beside the insertion of posterior papillary muscle [Tnormal posterior wall]; percentage of minimum and maximum thinning were calculated as [100−(Tmax/Tnormal posterior×100)] and [100−(Tmin/Tnormal posterior×100)], respectively; maximum diameter between remodeled and contralateral normal wall, passing through the cavity center [LV DR]; maximum diameter between normal ventricular walls, perpendicular to LV DR and passing nearest the center of the ventricular cavity [LV DN]; sphericity index [LV DR/DN]) were measured by 2 observers blinded to the treatment allocation. Measurements were performed in all affected slices and the means±SEM. were calculated for each animal.
5
High-Resolution MRI and DWI Acquisition
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MRI was collected from a General Electric 1.5 Tesla using an eight-channel head coil. The imaging protocol consisted of: 3D T1-weighted high-resolution images using a Fast Spoiled Gradient Echo sequence [TR/TE/TI = 11.2/4.2/450 ms; flip-angle = 12° ; FoV = 250 mm; acquisition matrix = 256 × 256; slice thickness = 1 mm] and Diffusion weighted images (DWI) using a single-shot echo planar imaging sequence [TR/TE = 12000/96.1 ms; FoV = 307 mm; acquisition matrix = 128 × 128; slice thickness = 2.4 mm; NEX = 3]. DWI was acquired along 25 non-coplanar directions with a b-value of 900 s/mm2 and 1 image with no diffusion sensitization, i.e. b0 image.
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