Kinetics of hydrolysis of prodrug into active doxorubicin were measured by dissolving Doxorubicin prodrug 4 at 172 nM in phosphate buffered saline of different pH (pH = 6.0, 6.5, 7.0, 7.5). Cleavage of prodrug was monitored by liquid chromatography/mass spectrometry on an Agilent 1290/6140 Ultra High Performance Liquid Chromatography/Mass Spectrometer (UHPLC/MS) consisting of a 1290 Infinity LC binary pump interfaced with an Agilent 1290 Diode Array Detector, an Agilent 1290 Infinity Autosampler, an Agilent Thermostat Column Compartment, and an Agilent 6140 Quadrupole MSD system. Integration of the LCMS peak corresponding to the doxorubicin was measured and confirmed with loss of the prodrug peak.
1290 diode array detector
Manufactured by Agilent Technologies
Sourced in United States
The 1290 Diode Array Detector is a high-performance liquid chromatography (HPLC) detector manufactured by Agilent Technologies. It is designed to provide sensitive and accurate detection of a wide range of analytes in complex samples. The core function of the 1290 Diode Array Detector is to measure the absorbance of the analytes at multiple wavelengths simultaneously, allowing for the identification and quantification of compounds based on their unique spectral signatures.
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3 protocols using 1290 diode array detector
1
Kinetics of Doxorubicin Prodrug Hydrolysis
2
Quantitative HPLC Analysis of RFX
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The concentration of RFX was analyzed using an Agilent 1260 Infinity HPLC instrument (Agilent, Santa Clara, CA, USA) equipped with an Agilent 1290 Diode Array Detector and an Agilent column (TC-C18, 4.6 × 250 mm, 5 μm). The mobile phase consisted of 3.16 g/L of ammonium formate (pH 7.2 ± 0.05) and a methanol–acetonitrile mixture (1:1, v/v) at a volume ratio of 20 to 80. The analysis was conducted at a detection wavelength of 276 nm with a flow rate of 1.0 mL/min and an injection volume of 20 μL. The collected data were analyzed using Agilent OpenLab CDS LC 1260 software.
3
HPLC Analysis of Virus-Like Particles
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