8iso pge2

Methanol, acetonitrile,
isopropanol (all
LC-MS grade), formic acid (98–100%), and silver nitrate (AgNO₃, >99.8%) were purchased from Merck (Darmstadt, Germany).
Prostaglandin standards (PGE₂, PGD₂, Δ12-PGD₂, 8iso-PGE₂, 15(R)-PGE₂, ent-PGE₂, 11β-PGE₂, 5-trans-PGE₂, 15(R)-PGD₂, PGD₂-d₉, and PGF_2a-d₉) were purchased from Cayman Chemicals (MI, USA). Octadecanoyl
(18,18,18–d₃)-l-carnitine-HCl (C18-carnitine-d₃), arachidonic acid-d₈, and 10(Z),13(Z)-monononadecadienoin
(MG 19:2) were purchased from Larodan AB (Solna, Sweden). Lysophosphatidylcholine
19:0 (LPC 19:0), phosphatidylcholine 11:0/11:0 (PC 11:0/11:0), and
oleic acid-d₉ were purchased from Merck. Monoisotopic silver
(¹⁰⁷Ag) was purchased from Trace Sciences International
(Richmond Hill, ON, Canada), and a solution was prepared previously
in-house.^{33 (link)} Deionized water was obtained
from an in-house purification system (Millipore, Merck).

Methanol, NaOH, KOH, ethanol, heptane, acetonitrile and ethyl acetate (analytical grade) were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). The formic acid and HCl were from Panreac (Barcelona, Spain) and Ultrapure H₂O was generated on a milliQ system from Millipore.
Lipid oxidative damage was determined by the analysis of prostaglandines (PG), isoprostanes (IsoP), neuroprostanes (NeuroP), dihomo-IsoP, and dihomo-isofurans (dihomo-IsoF) using ultra high-performance liquid chromatography mass spectrometry (UPLC-MS/MS) (Waters Acquity UPLC-XevoTQ system, Milford, MA, USA). The analysis was carried out on different biological matrices: retina, plasma and urine.
The prostaglandin standard PGE₂, PGF_2α, the IsoP standards 8-iso-PGE₂, 8-iso-15-keto-PGE₂, 8-iso-PGF_2α (also named 15-F_2t-IsoP), 8-iso-15-keto-PGF_2α, 8-iso-15(R)-PGF_2α, 5-iso-PGF_2α–VI, 2,3-dinor-8-iso-PGF_2α, 1a,1b-dihomo-PGF_2α, and deuterated internal standard PGF_2α-d₄ were obtained from Cayman Chemical Company (Ann Arbor, MI, USA) (Figure 1). The standards 10-epi-10-F_4t-NeuroP, 4(RS)-4-F_4t-NeuroP, 14(RS)-14-F_4t-NeuroP, 17-F_2t-dihomo-IsoP, Ent-7(RS)-7-F_2t-dihomo-IsoP, 17-epi-17-F_2t-dihomo-IsoP, 17(RS)-10-epi-SC-Δ¹⁵-11-dihomo-IsoF, 7(RS)-ST-Δ⁸-11-dihomo-IsoF were synthesized at the Institute of Biomolecules Max Mousseron (IBMM) (Montpellier, France) by Professor Durand’s team [15 (link)] (Figure 1).