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Diethyl pyrocarbonate depc water

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Diethyl pyrocarbonate (DEPC) water is a type of purified water used in laboratories. It is treated with diethyl pyrocarbonate, a chemical that inactivates RNase enzymes, which can degrade RNA samples. DEPC water is commonly used in RNA-based experiments to ensure the integrity of RNA molecules.

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Lab products found in correlation

Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions) Betaine, by Merck Group (2 mentions) Evagreen, by Avantor (2 mentions) Chikv s 27, by BEI Resources (2 mentions) Six primers, by Integrated DNA Technologies (2 mentions) Sodium citrate, by Innovative Research (2 mentions) Bst3.0 polymerase, by New England Biolabs (2 mentions) Deoxynucleotide triphosphates dntps, by Agilent Technologies (2 mentions) Isothermal buffer 2, by New England Biolabs (2 mentions) Accuspan plasma, by LGC (2 mentions) Accuspan linearity panel, by LGC (2 mentions) Sphi and psti restriction enzymes, by New England Biolabs (2 mentions)

Spelling variants of this product

DEPC water (26 citations) UltraPure diethyl pyrocarbonate (DEPC)-treated water (3 citations) Diethyl pyrocarbonate (DEPC)- (2 citations)

2 protocols using diethyl pyrocarbonate depc water

1

RT-LAMP Assay for Viral Detection

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Reagents necessary for the RT-LAMP assay included six primers (Integrated DNA Technologies, Skokie, IL), Bst 3.0 polymerase (NEB, Ipswich, MA), deoxynucleotide triphosphates (dNTPs) (Agilent Technologies, Santa Clara, CA), isothermal buffer II (NEB, Ipswich, MA), betaine (Millipore Sigma, Burlington, MA), EvaGreen (VWR International, Radnor, PA), ROX (Thermo Fisher Scientific, Waltham, MA), diethyl pyrocarbonate (DEPC) water (Invitrogen, Carlsbad, CA), and human whole blood collected in sodium citrate (Innovative Research, Novi, MI).
Template used in the experiments below included purified genomic RNA from HIV-1 (ATCC, Manassas, VA), non-infectious HIV-1 virus diluted in AccuSpan plasma (AccuSpan Linearity Panel, SeraCare Life Sciences, Milford, MA), purified genomic RNA from dengue virus (DENV) type 1 (BEI resources, Manassas, VA), and purified RNA from chikungunya virus (CHIKV) S-27 (BEI resources, Manassas, VA). SphI and PstI restriction enzymes (NEB, Ipswich, MA) and phosphate buffered saline (PBS) (Thermo Fisher Scientific, Waltham, MA) are additional reagents used.
Phillips E.A., Moehling T.J., Ejendal K.F., Hoilett O.S., Byers K.M., Basing L.A., Jankowski L.A., Bennett J.B., Lin L.K., Stanciu L.A, & Linnes J.C. (2019). Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples. Lab on a chip, 19(20), 3375-3386.
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2

RT-LAMP Assay for Viral Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents necessary for the RT-LAMP assay included six primers (Integrated DNA Technologies, Skokie, IL), Bst 3.0 polymerase (NEB, Ipswich, MA), deoxynucleotide triphosphates (dNTPs) (Agilent Technologies, Santa Clara, CA), isothermal buffer II (NEB, Ipswich, MA), betaine (Millipore Sigma, Burlington, MA), EvaGreen (VWR International, Radnor, PA), ROX (Thermo Fisher Scientific, Waltham, MA), diethyl pyrocarbonate (DEPC) water (Invitrogen, Carlsbad, CA), and human whole blood collected in sodium citrate (Innovative Research, Novi, MI).
Template used in the experiments below included purified genomic RNA from HIV-1 (ATCC, Manassas, VA), non-infectious HIV-1 virus diluted in AccuSpan plasma (AccuSpan Linearity Panel, SeraCare Life Sciences, Milford, MA), purified genomic RNA from dengue virus (DENV) type 1 (BEI resources, Manassas, VA), and purified RNA from chikungunya virus (CHIKV) S-27 (BEI resources, Manassas, VA). SphI and PstI restriction enzymes (NEB, Ipswich, MA) and phosphate buffered saline (PBS) (Thermo Fisher Scientific, Waltham, MA) are additional reagents used.
Phillips E.A., Moehling T.J., Ejendal K.F., Hoilett O.S., Byers K.M., Basing L.A., Jankowski L.A., Bennett J.B., Lin L.K., Stanciu L.A, & Linnes J.C. (2019). Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples. Lab on a chip, 19(20), 3375-3386.
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