Luria bertani agar la

Manufactured by Merck Group

Sourced in Germany

Luria-Bertani Agar (LA) is a common microbiological growth medium used for the cultivation and isolation of various bacterial species. It provides the necessary nutrients and solidifying agent for the growth of bacteria in a laboratory setting. The core function of LA is to support the growth and proliferation of bacterial cultures, which is essential for various applications in microbiology, biotechnology, and related fields.

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Lab products found in correlation

Mueller hinton broth 2 mhb2, by Merck Group (2 mentions) α pinene, by Merck Group (1 mentions) Baird parker agar plates, by Biomaxima (1 mentions) S aureus atcc, by American Type Culture Collection (1 mentions) S epidermidis, by American Type Culture Collection (1 mentions) Staphylococcus aureus atcc, by American Type Culture Collection (1 mentions) Mueller hinton broth (mhb), by Merck Group (1 mentions)

4 protocols using luria bertani agar la

Bacterial Strains and Volatile Organic Compounds

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The following strains of A. tumefaciens were used in this work: A. tumefaciens C58, isolated from cherry crown gall (USA), nopaline-type [25 (link)]; A. tumefaciens Chry5, isolated from Chrysanthemum crown gall (USA), chrysopine-type [26 (link)]. Bacteria were grown in liquid Luria–Bertani broth (LB) or on solid (1.5% w/v agar) Luria–Bertani agar (LA) (Sigma-Aldrich Chemie GmbH, Steinheim, Germany) at 28 °C.
The pure VOCs of the following classes were studied (Figure 1): alcohols (2-phenylethanol and isoamyl alcohol; purity of both is >99%); ketones (2-butanone, 2-pentanone, 2-octanone, unsaturated ketone, and norterpenoid β-ionone; all of them had >99% purity); and terpenes ((–)-limonene with purity > 96% and (+)-α-pinene with purity > 98%). All compounds were obtained from Sigma-Aldrich Chemie GmbH, Steinheim, Germany. VOCs were taken directly from the initial liquid preparation without dilution in a solvent.

Sidorova D.E., Skripka M.I., Khmel I.A., Koksharova O.A, & Plyuta V.A. (2022). Effects of Volatile Organic Compounds on Biofilms and Swimming Motility of Agrobacterium tumefaciens. Microorganisms, 10(8), 1512.

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Antimicrobial Activity of Propolis Extracts

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In the preliminary studies, the antimicrobial activity of ethanol extracts of propolis was tested against five reference strains of bacteria: Staphylococcus aureus ATCC 25923, S. aureus ATCC 29213, S. epidermidis ATCC 12228, Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 25922. The anti-staphylococcal potential of selected propolis samples was also investigated against sixteen MSSA and five MRSA isolates from patients with different infections (Table 7.). Bacteria were routinely grown on Luria-Bertani Agar (LA, Sigma Aldrich, Schnelldorf, Germany). The Minimum Inhibitory Concentration (MIC) was determined using liquid medium—Mueller-Hinton Broth 2 (MHB2, Sigma Aldrich) and for determination of Minimum Bactericidal Concentrations (MBC) the cells were transferred on the Baird Parker Agar plates (Biomaxima, Lublin, Poland). For biofilm formation TSB liquid medium supplemented with 2.5% of glucose was used.

Grecka K., Kuś P.M., Okińczyc P., Worobo R.W., Walkusz J, & Szweda P. (2019). The Anti-Staphylococcal Potential of Ethanolic Polish Propolis Extracts. Molecules, 24(9), 1732.

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Antibacterial Potential of Propolis

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The antimicrobial activity of ethanol extract of propolis was tested against four reference strains of bacteria: Staphylococcus aureus ATCC 25923, S. aureus ATCC 29213, S. epidermidis ATCC 12228, and S. epidermidis ATCC 35984 (American Type Culture Collection, Manassas, VA, USA). The anti-staphylococcal potential of the propolis sample was also investigated against six MSSA, and three MRSA isolates from patients with different infections (Table 2). S. epidermidis ATCC 35984, a strongly adherent, slime-producing strain, was employed as a model for biofilm studies. S. aureus and S. epidermidis strains were routinely grown on Luria-Bertani Agar (LA, Sigma Aldrich, Schnelldorf, Germany) and Brain Heart Infusion Agar (BHA, Becton Dickinson and Company, Franklin Lakes, NJ, USA). The Minimum Inhibitory Concentration (MIC) was determined using a liquid medium—Mueller–Hinton Broth 2 (MHB2, Sigma Aldrich, Poland). For biofilm formation, TBS liquid medium supplemented with 2.5% of glucose was used (Becton Dickinson and Company, Franklin Lakes, NJ, USA).

Grecka K., Xiong Z.R., Chen H., Pełka K., Worobo R.W, & Szweda P. (2020). Effect of Ethanol Extracts of Propolis (EEPs) against Staphylococcal Biofilm—Microscopic Studies. Pathogens, 9(8), 646.

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Antimicrobial Evaluation of Bacterial Extracts

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Five reference strains of bacteria, S. aureus ATCC 25923, S. aureus ATCC 29213, S. epidermidis ATCC 12228, P. aeruginosa ATCC 27853 and E. coli ATCC 25922, were applied for preliminary assessments of the antimicrobial potential of all produced BP and BB ethanolic extracts Subsequently anti-staphylococcal activity of selected extracts was evaluated against 6 MSSA (methicillin-susceptible Staphylococcus aureus) and 3 MRSA (methicillin-resistant Staphylococcus aureus) isolates from patients of the hospital of Medical University of Gdańsk, that suffered with different infections (Table 3). Bacteria were routinely grown on Luria-Bertani Agar (LA, Sigma Aldrich, Schnelldorf, Germany). The determination of the values of Minimum Inhibitory Concentration (MIC) was performed in Mueller-Hinton Broth (MHB, Sigma Aldrich) and for determination of Minimum Bactericidal Concentrations (MBC) the cells were cultivated on the selective Baird Parker Agar plates (Biomaxima, Lublin, Poland). The reference strain S. aureus ATCC 29213 was used for antimicrobial potential evaluation of both extracts and suspensions of selected products in kinetic time-kill assay.

Pełka K., Otłowska O., Worobo R.W, & Szweda P. (2021). Bee Bread Exhibits Higher Antimicrobial Potential Compared to Bee Pollen. Antibiotics, 10(2), 125.

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