Western blotting analysis was conducted as previously reported [40 (link)]. Anti-Runx2 (Cat No. WL03358, 1:500, WanleiBio, Shenyang, China), anti-MMP9 (Cat No. WL03096, 1:500, WanleiBio, China), anti-MMP13 (Cat No. WL04694, 1:500, WanleiBio, China), anti-Col10a1 (Cat No. AF6538, 1:500, Beyotime, Shanghai, China), anti-Caspase 3 (Cat No. WL02117, 1:500, WanleiBio, China), anti-Cleaved caspase 3 (Cat No. WL01992, 1:500, WanleiBio, China), anti-β-actin (Cat No. 20536-1-AP, 1:1000, Proteintech, Wuhan, China) primary antibodies were used.
Anti mmp9
Manufactured by Wanlei
Sourced in China
Anti-MMP9 is a laboratory reagent used to detect and quantify the presence of matrix metalloproteinase-9 (MMP-9) in biological samples. MMP-9 is an enzyme involved in the breakdown of extracellular matrix and has been implicated in various physiological and pathological processes. The Anti-MMP9 reagent can be used in various analytical techniques, such as ELISA, Western blotting, and immunohistochemistry, to measure MMP-9 levels in a wide range of research and clinical applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti runx2, by Wanlei (2 mentions)
Anti mmp13, by Wanlei (2 mentions)
Anti cleaved caspase 3, by Wanlei (1 mentions)
Anti β actin, by Proteintech (1 mentions)
Anti caspase 3, by Wanlei (1 mentions)
Anti alas1, by Proteintech (1 mentions)
Sirna alas1, by Cell Signaling Technology (1 mentions)
Anti mmp2, by Wanlei (1 mentions)
Anti pcna, by Cell Signaling Technology (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti c myc, by Abcam (1 mentions)
Recombinant human vegf a, by R&D Systems (1 mentions)
Anti alkbh5, by Abcam (1 mentions)
Vegfa neutralizing antibody, by R&D Systems (1 mentions)
Anti rac3, by Proteintech (1 mentions)
Anti twist1, by Wanlei (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti m6a, by Synaptic Systems (1 mentions)
Anti rbpj, by Beyotime (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
4 protocols using anti mmp9
1
Western Blot Analysis of Osteoarthritis Markers
2
Succinylacetone and Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Succinylacetone was purchased from MedChemExpress (Cat# HY-W010184). Antibodies were used to determine protein expression including anti-ALAS1 (Proteintech, Rosemont, IL, USA Cat# NBP1-91656), anti-MMP2 (Wanleibio, Shenyang, China Cat# WL03224), anti-MMP9 (Wanleibio, Shenyang, China, Cat# WL03206), anti-PCNA (Cell Signaling Technology, USA, Cat# 13110), anti-c-Myc (Abcam, Cambridge, MA, USA, Cat# ab32072), and anti-β-actin (Cell Signaling Technology, USA, Cat# 4967).
The small interfering RNA (siRNA) oligonucleotides [siRNA-NC: 5'-UUCUCCGAACGUGUCACGUTT-3' (sense) and 5'-ACGUGACACGUUCGGAGAATT-3' (anti-sense); siRNA-ALAS1#1: 5'-GGUGCAGUAAU GACUACCUTT-3' (sense) and 5'-AGG UAG UCAUUACUGCACCTT-3' (anti-sense); siRNA-ALAS1#2: 5'-GCAGACAUAAC AUCUACGUTT-3' (sense) and 5'-ACGUAGAUG UUAUGUCUGCTT-3' (anti-sense)] were constructed and provided by GenePharma (Shanghai, China).
The small interfering RNA (siRNA) oligonucleotides [siRNA-NC: 5'-UUCUCCGAACGUGUCACGUTT-3' (sense) and 5'-ACGUGACACGUUCGGAGAATT-3' (anti-sense); siRNA-ALAS1#1: 5'-GGUGCAGUAAU GACUACCUTT-3' (sense) and 5'-AGG UAG UCAUUACUGCACCTT-3' (anti-sense); siRNA-ALAS1#2: 5'-GCAGACAUAAC AUCUACGUTT-3' (sense) and 5'-ACGUAGAUG UUAUGUCUGCTT-3' (anti-sense)] were constructed and provided by GenePharma (Shanghai, China).
3
Antibody and Protein Sources for VEGFA Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant human VEGFA and VEGFA-neutralizing antibody were purchased from R&D Systems (Minneapolis, MN). Antibodies against METTL3 (#86132), FTO (#31687), AKT (#4685), p-AKT (#4060), and p65 (#8242) (in 1:1000 dilution) were purchased from Cell Signaling Technology (Beverly, MA); anti-ALKBH5 (# ab69325, 1:1000 dilution) was purchased from Abcam (Cambridge, UK); anti-m6A (#202003, 1:1000 dilution) was purchased from Synaptic Systems (Göttingen, Germany); anti-RAC3 (#16117-1, 1:500 dilution) was purchased from Proteintech (Rosemont, IL); anti-p-p65 (#WL02169), anti-MMP-9 (#WL03096), and anti-TWIST1 (#WL00997) (in 1:1000 dilution) were purchased from Wanleibio (Shenyang, China); anti-β-actin (#ab8224, 1:4000 dilution) was purchased from Sigma-Aldrich (St. Louis, MO); and goat anti-rabbit (#ZB-2301, 1:8000 dilution) and anti-mouse IgG (#ZB2305, 1:8000 dilution) were purchased from ZSGB-BIO (Beijing, China).
4
Western Blot Analysis of Notch Pathway
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After treatment and detection of total protein concentration with the BCA Protein Assay Kit (Epizyme Bio, Shanghai, China), total proteins were collected from cells for protein blotting experiments [22 (link),46 (link)]. Anti-Notch1 (1:300, Wanlei Bio, Shenyang, China), anti-p-Notch1 (1:300, Wanlei Bio, China), anti-Notch2 (1:300, Wanlei Bio, China), anti-RBPJ (1:1000, Beyotime Biotechnology, Shanghai, China), anti-Runx2 (1:300, Wanlei Bio, China), anti-Col2a1 (1:1000, Beyotime Biotechnology, Shanghai, China), anti-BMP2 (1:300, Wanlei Bio, China), anti-MMP9 (1:300, Wanlei Bio, China), anti-MMP13 (1:300, Wanlei Bio, China), anti-GAPDH (1:1000, Proteintech, Wuhan, China) primary antibodies were used.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!