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S propranolol hydrochloride

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S)-(−)-propranolol hydrochloride is a chemical compound used in laboratory research. It is a hydrochloride salt of the beta-blocker drug propranolol, which acts as an antagonist of beta-adrenergic receptors. The compound is utilized in various scientific investigations, but a detailed description of its specific functions or intended uses is not provided to maintain an unbiased and factual approach.

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Lab products found in correlation

Clonidine, by Merck Group (2 mentions) Clonidine hydrochloride, by Merck Group (2 mentions) Lidocaine hydrochloride monohydrate, by Merck Group (2 mentions) Lidocaine, by Merck Group (2 mentions) Sch23390 sch 23390 hydrochloride, by Bio-Techne (2 mentions) Carazolol, by Merck Group (1 mentions) Fastlab, by GE Healthcare (1 mentions) Pindolol, by Merck Group (1 mentions) Cl316 243, by Bio-Techne (1 mentions) Vybrant apoptosis assay kit 5, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Propranolol hydrochloride Propranolol hydrochloride (propranolol) S-propranolol Propranolol Hydrochlorides

5 protocols using s propranolol hydrochloride

1

Microinfusion of Receptor Antagonists

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For microinfusions, the concentrations used were 21.1 mM (6.25 μg μl−1) for β-adrenoceptor antagonist propranolol [(S)-(−)-propranolol hydrochloride, 295.80 g mol−1; Sigma-Aldrich), 3.1 mM (1 μg μl−1) for D1/D5 receptor antagonist SCH23390 (SCH 23390 hydrochloride, 324.24 g mol−1; Tocris) and 2% w/v for voltage-gated sodium channel blocker lidocaine (lidocaine hydrochloride monohydrate, 288.81 g mol−1; Sigma-Aldrich). α2-adrenoceptor agonist clonidine (clonidine hydrochloride, 266.55 g mol−1; Sigma-Aldrich) was administered i.p. at a dose of 50 μg kg−1 of body weight. We used 0.9% NaCl (saline) in H2O as a vehicle and for control infusions. Both vehicle and drug solutions were stored in 100 μl aliquots at −20°C until use.
Takeuchi T., Duszkiewicz A.J., Sonneborn A., Spooner P.A., Yamasaki M., Watanabe M., Smith C.C., Fernández G., Deisseroth K., Greene R.W, & Morris R.G. (2016). Locus coeruleus and dopaminergic consolidation of everyday memory. Nature, 537(7620), 357-362.
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2

Radiolabeled PET Tracers and Ligands Synthesis

Cited 1 time
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2-[18F]fluoro-2-deoxy-D-glucose ([18F]FDG) was prepared in-house using a fully automated cassette-based synthesizer (FASTlab, GE Healthcare, Uppsala, Sweden) within the clinical routine production at the Vienna General Hospital, Austria. [11C]SNAP-7941 was synthesized as reported elsewhere using an automated module (TRACERlab FC X Pro, GE Healthcare, Uppsala, Sweden) (25 (link)). All PET-tracers were physiologically formulated and quality-controlled prior to administration. The adrenergic receptor beta ligands carazolol, pindolol and (S)-propranolol hydrochloride were purchased from Sigma-Aldrich (St. Louis, USA). The ADRB3 agonist CL 316,243 was purchased from Tocris Bioscience (Bristol, UK). The unlabeled compounds FE@SNAP and SNAP-7941 were synthesized at the Department of Pharmaceutical Chemistry and at the Department of Organic Chemistry (University of Vienna, Austria). The radioligands 5,7-[3H](-)CGP-12177 and [125I](-)Iodocyanopindolol were purchased from PerkinElmer, Inc. (Waltham, USA). All other reagents and cell culture supplies were purchased from standard commercial sources.
Balber T., Benčurová K., Kiefer F.W., Kulterer O.C., Klebermass E.M., Egger G., Tran L., Wagner K.H., Viernstein H., Pallitsch K., Spreitzer H., Hacker M., Wadsak W., Mitterhauser M, & Philippe C. (2019). In vitro Radiopharmaceutical Evidence for MCHR1 Binding Sites in Murine Brown Adipocytes. Frontiers in Endocrinology, 10, 324.
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3

Microinfusion of Receptor Antagonists

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For microinfusions, the concentrations used were 21.1 mM (6.25 μg μl−1) for β-adrenoceptor antagonist propranolol [(S)-(−)-propranolol hydrochloride, 295.80 g mol−1; Sigma-Aldrich), 3.1 mM (1 μg μl−1) for D1/D5 receptor antagonist SCH23390 (SCH 23390 hydrochloride, 324.24 g mol−1; Tocris) and 2% w/v for voltage-gated sodium channel blocker lidocaine (lidocaine hydrochloride monohydrate, 288.81 g mol−1; Sigma-Aldrich). α2-adrenoceptor agonist clonidine (clonidine hydrochloride, 266.55 g mol−1; Sigma-Aldrich) was administered i.p. at a dose of 50 μg kg−1 of body weight. We used 0.9% NaCl (saline) in H2O as a vehicle and for control infusions. Both vehicle and drug solutions were stored in 100 μl aliquots at −20°C until use.
Takeuchi T., Duszkiewicz A.J., Sonneborn A., Spooner P.A., Yamasaki M., Watanabe M., Smith C.C., Fernández G., Deisseroth K., Greene R.W, & Morris R.G. (2016). Locus coeruleus and dopaminergic consolidation of everyday memory. Nature, 537(7620), 357-362.
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4

Propranolol Cytotoxicity Assay Protocol

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Cells (1 × 104) were plated on flat-bottom 96-well plates and subsequently exposed to 0–400 μM (S)-(−)-propranolol hydrochloride (Sigma-Aldrich) for 24–48 hours at 37°C.7 (link),38 (link) After incubation, the Cell Counting Kit-8 cytotoxicity assay (Dojindo Molecular Technologies) was subsequently performed according to the manufacturer’s protocol. An ELx800 96-well spectrometer (BioTek Instruments) was used to perform all absorbance measurements.
Shepard M.J., Bugarini A., Edwards N.A., Lu J., Zhang Q., Wu T., Zhuang Z, & Chittiboina P. (2018). Repurposing propranolol as an antitumor agent in von Hippel-Lindau disease. Journal of neurosurgery.
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5

Propranolol Induces Cell Death

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The 786-O cells were cultured in the presence of 0–200 μM (S)-(−)-propranolol hydrochloride (Sigma-Aldrich) for 24–48 hours at 37°C. The Vybrant Apoptosis Assay Kit #5 (Hoechst 33342/propidium iodide, ThermoFisher Scientific) was then used to determine the percentage of necrotic and apoptotic cells in culture according to the manufacturer’s specification.
Shepard M.J., Bugarini A., Edwards N.A., Lu J., Zhang Q., Wu T., Zhuang Z, & Chittiboina P. (2018). Repurposing propranolol as an antitumor agent in von Hippel-Lindau disease. Journal of neurosurgery.
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