In EBUS-GS TBB, we used video bronchoscopes (BFp-260F, 4.0-mm outer diameter and BF1T-260, 5.9-mm outer diameter; Olympus, Tokyo, Japan) with an ultrasound scanner (EU-ME-1; Olympus) for the EBUS-GS biopsies. We used guide sheath kits with two sizes (K-201 and K-203 unit; Olympus). Each guide sheath kit consisted of a guide sheath, forceps, and a cytology brush. To detect the target lesion, we used radial endobronchial ultrasound probes (UM-S20-17S, 1.7-mm outer diameter and UM-S20-20R, 2.0-mm outer diameter; Olympus). In the CTBB group, we used several types of bronchoscopes for biopsy (BF260, BF6C260, BFp260F, and BF1T260; Olympus), disposable biopsy forceps (FB-231D; Olympus), and disposable cytology brushes (BC-202D-2010; Olympus).
Bc 202d 2010
Manufactured by Olympus
Sourced in Germany, United Kingdom, Japan
The BC-202D-2010 is a compact, automated hematology analyzer designed for clinical laboratory use. It is capable of performing complete blood count (CBC) and 3-part differential measurements on whole blood samples. The analyzer utilizes impedance-based technology for cell counting and sizing.
Automatically generated - may contain errors
Lab products found in correlation
Fb 231d, by Olympus (3 mentions)
Bf 1t260, by Olympus (3 mentions)
Um s20 20r, by Olympus (2 mentions)
Bf p260f, by Olympus (2 mentions)
Bf 260, by Olympus (1 mentions)
Bf 6c260, by Olympus (1 mentions)
Um s20 17s, by Olympus (1 mentions)
K 201, by Olympus (1 mentions)
Eu me1, by Olympus (1 mentions)
Bf 1tq290, by Olympus (1 mentions)
Sg 201 c, by Olympus (1 mentions)
Cryo2, by Erbe (1 mentions)
7 protocols using bc 202d 2010
1
EBUS-GS and CTBB Biopsy Techniques
2
Flexible Bronchoscopy with EBUS and Cryo Biopsy
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The procedures used in this study were described previously [14 (link)]. A flexible fiber bronchoscope (BF-1TQ290; Olympus, Tokyo, Japan), 20-MHz radial EBUS probe (UM-S20-20R; Olympus), guide sheath (SG-201C; Olympus), brush (BC-202D-2010; Olympus), forceps (FB-231D; Olympus), and 1.9 mm cryo probe (CRYO2; ERBE, Tuebingen, Germany) were employed [14 (link)]. Thrombin (Liquid Thrombin MOCHIDA Softbottle 10,000; Mochida Pharmaceutical, Tokyo, Japan) and balloon catheter (B5-2C; Olympus) were prepared in case of mild or severe bleeding [14 (link)]. Local anesthesia with 1% lidocaine for nebulizing, 2% lidocaine bolus to the bronchus, intravenous injection of 2–2.5 mg of midazolam, and intra-muscular injection of 35 mg pethidine hydrochloride for conscious sedation were used during the procedures [9 (link), 14 (link)]. The blood pressure, oxygen saturation, pulse rate, and electrocardiography of all patients were monitored in this study [9 (link), 14 (link)].
3
Bronchoscopic Tumour Sampling Procedure
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The same pulmonologist with extensive bronchoscopy experience performed all procedures via the transnasal or transoral route using a variety of video bronchoscopes (models BF-1T260, F260, and 6C260; Olympus; Tokyo, Japan) under local anaesthesia (2% lidocaine spray) and mild conscious sedation with midazolam. Additional doses of lidocaine and midazolam were added during the procedures at the physician’s discretion. After visualising the vocal cords, the trachea and all bronchial trees, including the subsegmental bronchi, were inspected. Bronchial brushing was performed without fluoroscopic guidance using disposable cytology brushes with a covered sheath (BC-202D-2010, Olympus). The brush was passed through the working channel of the bronchoscope while in its sheath and advanced as far as possible into the subsegmental bronchus that was considered to contain the target tumour based on chest CT findings. At this point, the brush and sheath were retracted 3–5 cm, and then the brush was pushed out of the covered sheath and moved back and forth over the lesion several times. After removing the brush from the working channel, 10–20 mL sterile saline were instilled through the working channel in a wedged position in the involved segment, and the washing samples were aspirated. This procedure was repeated two or three times.
4
Airway Epithelial Cell Sampling in Children
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Children aged ≤16 years scheduled for routine ear nose and throat operations under anaesthesia were recruited between January 2011 and April 2013. Exclusion criteria included upper respiratory tract infection in the previous week and hereditary bleeding conditions. A lifetime history of asthma, eczema and hayfever, receipt of current asthma medications and exposure to parental smoking at home was obtained by a researcher-administered questionnaire. A bronchoscopy cytology brush (10-mm disposable cytology brush, BC 202D-2010, Olympus, Southend-on-Sea, Essex, UK) was used to obtain “blind” bronchial brushings to obtain airway epithelial cells. Ethical approval for the study was granted by the North of Scotland Medical Research Ethics Committee (approval numbers 09/S0802/122 and 12/NS/0107) and informed patient consent was obtained.
5
Bronchial Sampling Methods for Research
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Bronchoabsorption exudate - Lung lining fluid was collected using Accuwick Ultra membrane (Pall, USA) which is a specialised fibrous hydroxylated polyester absorptive matrix paper. A pre-cut Accuwick strip was guided through the K-203 guide sheath within the bronchoscope using Olympus forceps (FB-231D, oval, fenestrated swing jaw biopsy forceps, working length 1150 mm, and an outer diameter of 2.0 mm). Strips were left to absorb bronchial secretions for up to 2 min before they were withdrawn back through the bronchoscope.
Bronchial lavage - BAL collection was performed using 4 x 60 mL aliquots (240mL in total) of 0.9 % saline (Nebusal 7 %) pre-warmed. Each aliquot was immediately recovered by gentle negative pressure using a suction pump or directly into a syringe and collected into a container cooled in ice.
Bronchial brushing - Utilizing an endobronchial disposable Olympus cytology brush (BC-202D-2010, 2 mm brush diameter, 10 mm brush length, working length 1150 mm) bronchial brushings were obtained for microarray analysis. A bronchial brush was guided through the K-203 guide sheath within the bronchoscope. Once in position, the brush was gently rotated to collect mucosal cells. The brush was then removed through the guide sheath.
Bronchial lavage - BAL collection was performed using 4 x 60 mL aliquots (240mL in total) of 0.9 % saline (Nebusal 7 %) pre-warmed. Each aliquot was immediately recovered by gentle negative pressure using a suction pump or directly into a syringe and collected into a container cooled in ice.
Bronchial brushing - Utilizing an endobronchial disposable Olympus cytology brush (BC-202D-2010, 2 mm brush diameter, 10 mm brush length, working length 1150 mm) bronchial brushings were obtained for microarray analysis. A bronchial brush was guided through the K-203 guide sheath within the bronchoscope. Once in position, the brush was gently rotated to collect mucosal cells. The brush was then removed through the guide sheath.
6
Cytological Slide Preparation Protocol
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These procedures have been described in our previous studies.10 (link),12 (link),13 (link) First, a
straight brush (BC-202D-2010; Olympus, Tokyo, Japan) for BB was used for
smearing the slides. Then, we put the brush into a BD CytoRich™ Red preservative
(Cytyc Corporation, Boxborough, MA, USA) bottle. Finally, a BALF sample in 0.9%
saline was added to fill the bottle.
straight brush (BC-202D-2010; Olympus, Tokyo, Japan) for BB was used for
smearing the slides. Then, we put the brush into a BD CytoRich™ Red preservative
(Cytyc Corporation, Boxborough, MA, USA) bottle. Finally, a BALF sample in 0.9%
saline was added to fill the bottle.
7
Flexible Bronchoscopy for Diagnostic Sampling
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Flexible bronchoscopy using conventional (BF 1T260, Olympus, Tokyo, Japan) and thin (BF P260F, Olympus) bronchoscopes was performed via the oral route under sedation by intramuscular hydroxyzine and intravenous midazolam or propofol. Bronchial brushing was carried out using a disposable cytology brush (⌽1.8 mm; BC-202D-2010, Olympus). Transbronchial biopsy was performed using biopsy forceps (⌽1.9 mm [Endo Jaw FB-231D, Olympus], ⌽1.8 mm [KW1811S, PENTAX Medical, Montvale, New Jersey], or ⌽1.5 mm [KW1511S, PENTAX Medical]). We defined conventional biopsy as transbronchial biopsy without using EBUS guidance to distinguish from EBUS-guided transbronchial biopsy. During the procedure, continuous pulse oximetry was routinely used to monitor oxygen saturation and pulse rate. When necessary, oxygen was delivered to the subject via nasal cannula. Blood pressure was intermittently measured.
Secretions in the bronchus were aspirated for cytologic and microbiologic examinations. The lobar bronchus was washed with 1 or 2 10-mL aliquots of saline. Bronchoal-
Secretions in the bronchus were aspirated for cytologic and microbiologic examinations. The lobar bronchus was washed with 1 or 2 10-mL aliquots of saline. Bronchoal-
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