The mouse model of APAP induced acute liver injury was performed as previously described (You 2013 (link)). The mice were fasted overnight for approximately 16 h prior to intraperitoneal (i.p.) injection of phosphate buffered solution (PBS) or APAP (300 mg/kg, dissolved in warm PBS, Sigma, USA) at 9 a.m. The samples were collected after 8 h at 5 p.m. and 24 h at 9 a.m. next day. Blood was collected by retro-orbital puncture. Sera samples were collected for measurement of serum ALT and aspartate aminotransferase (AST) levels using an automated chemistry analyzer (Beckman AU5800, BECKMAN COULTER, USA). Liver samples were obtained at various time points after APAP challenge and paraffin-embedded tissue sections were prepared and stained with H&E. The Src family kinase selective inhibitor PP2 (Selleck, China) was injected to the mice intraperitoneally (1.5 mg/kg, dissolved in ethanol) at half an hour prior to APAP-treatment.
Beckman au5800
Manufactured by Beckman Coulter
Sourced in United States, Japan
The Beckman AU5800 is a high-throughput, fully automated clinical chemistry analyzer designed for use in clinical laboratories. It is capable of performing a wide range of routine and specialized clinical chemistry tests, including colorimetric, enzymatic, and immunoassay analyses. The AU5800 features advanced technology and automation to deliver accurate and reliable results, with the ability to process a high volume of samples efficiently.
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Xn 9100, by Sysmex (1 mentions)
Mab 4e6, by Mercodia (1 mentions)
Hem 7200, by Omron (1 mentions)
Lc 20ad, by Shimadzu (1 mentions)
Gpo pap method, by Beckman Coulter (1 mentions)
Au5800, by Roche (1 mentions)
Cobas 8000, by Roche (1 mentions)
Cba software, by BD (1 mentions)
Cba human th1 th2 cytokine kit 2, by BD (1 mentions)
Cd3 pc5, by BD (1 mentions)
Cd4 pe, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Hem 7117, by Omron (1 mentions)
Fossomatic 4000, by Foss (1 mentions)
Cobas e602, by Roche (1 mentions)
Beckman dxl800, by Beckman Coulter (1 mentions)
Facscanto 2, by BD (1 mentions)
Au5800, by Beckman Coulter (1 mentions)
24 protocols using beckman au5800
1
APAP-Induced Acute Liver Injury in Mice
2
Automated Biochemical Analysis for Kidney Function
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The Beckman AU5800 automatic biochemical analyzer (Beckman Coulter Corporation, Tokyo, Japan) was used to measure the serum concentrations of albumin (ALB), triglyceride (TG), creatinine, β2-microglobulin (β2-MG), Cystatin C (CysC). The estimated glomerular filtration rate calculation was based on the Modification of Diet in Renal Disease (MDRD) deformation formula published in 2006 by the Journal of the Nephropathy Association. Twenty-four-hour urine protein quantification
3
Blood Lipid Profiling Protocol
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Early in the morning on the day after admission, 5 mL of peripheral venous blood was drawn from all patients in a fasted state. The blood samples were centrifuged for 5 min in a centrifugation machine at a centrifuge radius of 10 cm and rotating speed of 3,000 revolutions/min. An automatic biochemical analyzer (Beckman AU5800; Beckman Coulter, Inc. Brea, CA, USA) was used to determine the serum levels of TC, TG, LDL-C, HDL-C, apoA-I, apoB, and Lp(a). The serum levels of TC, TG, LDL-C, and HDL-C were also measured using an enzyme-linked immunosorbent assay, while those of apoA-I, apoB, and Lp(a) levels were also measured using an immunoturbidimetric method. The kits were purchased from Shanghai Langdon Company (Blood Lipid Biochemical Test Kit; Shanghai, China), and the measurements were carried out strictly in accordance with the manufacturer’s instructions.
4
Measurement of Cardiometabolic Biomarkers
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The total cholesterol, triglyceride, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting blood glucose, Scr, and uric acid were measured by BECKMAN AU5800 biochemical analyzer (Beckman Coulter Inc., Brea, California, USA) . Diabetes mellitus was defined as a previous definitive diagnosis of diabetes mellitus or a fasting blood glucose level of ≥7.0 mmol/l [25 (link)].
5
Resting State Blood Analysis
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Patients were at a resting state during the early morning when blood samples were collected. The blood samples were tested using an XE-5000 automated fluorescence flow cytometer (Sysmex, Kobe, Japan) and a Beckman AU5800 (Beckman Coulter, Brea, CA, USA) automatic blood analyzer. All samples were tested within two hours of blood sampling.
6
Serum and Salivary Creatinine Measurement
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Serum and salivary creatinine levels were measured using a modification of Jaffe’s method,10 (link) and urea levels were estimated using the urease method.11 (link) Measurement was performed using commercially available kits (Beckman Coulter Diagnostics) and an autoanalyzer (Beckman AU 5800, Beckman Coulter Inc., Brea, CA). These methods have been used for saliva in earlier studies.12 (link),13 (link)
7
Liver Function Assessment for DMF Exposure
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GE LOGIQ 400 (GE Company, Fairfield, CT, USA) was applied to distinguish parenchymal liver diseases, liver cirrhosis and fatty liver. Five millilitres of venous blood were donated by all the volunteers, and immediately centrifuged at 1600 g for 10 min (blood cells and serum were separated and stored at −80 °C). The biochemical measurements were collected using a Beckman AU 5800 (Beckman Coulter, Inc., Brea, CA, USA); alanine aminotransferase (ALT) levels of more than 40 U/L were defined as DMF-induced abnormal liver function (subjects were excluded who drank 3 days before the liver function assessment), otherwise ALT levels of no more than 40 U/L were defined as normal liver function. We named the “workers with DMF-induced abnormal liver function” and normal liver function as “cases” and “controls”, respectively. The DMF concentrations were measured strictly according to the Specifications of Air Sampling for Hazardous Substances Monitoring in the Workplace (GBZ 159-2004), and the 8 h-TWA (time-weighted average) concentration was calculated. The current threshold limit value (TLV) of DMF in the workplace is 20 mg/m3.
8
Comprehensive Clinical Biomarker Profiling
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Laboratory results, physical examination findings and medical records of all subjects were obtained through the laboratory information system (LIS) and hospital information system (HIS) of PUMCH. The SBP and DBP could be available in the HIS system. White blood cell counts (WBC), red blood cell counts (RBC), hemoglobin (Hb), lymphocyte counts (L), neutrophil counts (N), platelet counts (PLT) were measured with XN-9100 (Sysmex Corporation, Japan). Neutrophil-to-lymphocyte ratio (NLR) was calculated by dividing the absolute neutrophil counts by the absolute lymphocyte counts, whilst platelet-to-lymphocyte ratio (PLR) was calculated by dividing the platelet counts by the absolute lymphocyte counts. Hemoglobin A1c (HbA1c) was measured using the D-100 hemoglobin testing system (Bio-Rad, USA). Serum glucose (Glu), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) were measured with Beckman AU 5800 (Beckman Coulter, USA).
9
Cardiometabolic Risk Profiling Protocol
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During each clinical examination, participants were scheduled to arrive to the clinic between 8 and 10 am having fasted overnight. Participants were interviewed at each visit using a standard protocol to collect demographic information (age, sex, body mass index, waist-to-hip ratio, smoking status, medical history, and residential address). After resting for 5 minutes, averages of 3 readings of heart rate and right upper arm BP were obtained in a sitting position using an autonomic sphygmomanometer (HEM-7200; Omron, Japan). Concentrations of high-sensitivity CRP (C-reactive protein; hs-CRP) and lipid profiles, including apoA-I, total cholesterol, HDL-C, LDL (low-density lipoprotein) cholesterol (LDL-C), and triglycerides, were measured by automatic analyzer (Beckman AU5800; Beckman Coulter) at the clinical laboratory of Peking University First Hospital. Plasma oxidized LDL levels were analyzed using a monoclonal antibody, mAb-4E6 (Mercodia AB, Uppsala, Sweden), with an interassay coefficient of variation of 5.5% at 8.5 mU/L.
10
Lipid Profile Assessment in TCM
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During the experiment, nine patients after YTD treatment finished the essential procedures for sampling fasting venous blood. Biochemical measurements of plasma glucose, total cholesterol, triglyceride, lipoprotein (a), low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and uric acid were conducted in the central laboratory of Jiangsu Province Hospital of Traditional Chinese Medicine (Nanjing, China). The biochemical parameters were analyzed using an automatic analyzer (Beckman AU5800; Beckman Coulter, USA). Lipoprotein (a) is a useful indicator in patients with cerebrovascular disease and atherosclerosis.
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