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Goat anti guinea pig cy3

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Goat anti-guinea pig Cy3 is a secondary antibody conjugated with the Cy3 fluorescent dye. It is designed to detect and bind to guinea pig primary antibodies, allowing for their visualization in various immunoassay techniques.

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Lab products found in correlation

Goat anti rabbit cy3, by Jackson ImmunoResearch (3 mentions) Goat anti rabbit fitc, by Jackson ImmunoResearch (1 mentions) Chicken anti gfp, by Abcam (1 mentions) Rabbit anti cgrp, by Immunostar (1 mentions) Axr confocal microscope, by Nikon (1 mentions)

3 protocols using goat anti guinea pig cy3

1

Quantification of Immunostaining Signals

Cited 4 times
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Immunostaining was performed as described [37 (link)]. The following antibodies were used: guinea pig anti-IR21a [19 (link)] (1:100), guinea pig anti-IR93a [18 (link)] (1:100), rabbit anti-IR25a [26 (link)] (1:100), chicken anti-GFP (1:500; Abcam), goat anti-guinea pig Cy3 (1:100; Jackson ImmunoResearch), goat anti-rabbit Cy3 (1:100; Jackson ImmunoResearch), goat anti-rabbit FITC (1:100; Jackson ImmunoResearch), goat anti-chicken FITC (1:500; Invitrogene).
To quantify immunostaining, the center of each soma was determined by NIS Elements Viewer and outlined based on GFP signals (Fig 4) or IR21a signals (S8 Fig). The mean intensity was quantified using ImageJ (Analyze>Measure) and subtracted by the background intensity. The normalized fluorescence was quantified as the fluorescent intensity was divided by the average intensity of the corresponding fluorescent intensity in wild type animals at 72 hr AEL.
Tyrrell J.J., Wilbourne J.T., Omelchenko A.A., Yoon J, & Ni L. (2021). Ionotropic Receptor-dependent cool cells control the transition of temperature preference in Drosophila larvae. PLoS Genetics, 17(4), e1009499.
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2

Immunohistochemical Profiling of Sensory Neurons

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Brain slices were frozen sectioned at 5 μm thickness and incubated with primary antibodies at 4°C overnight, followed by secondary antibody incubation at room temperature for 1 hour. Images were acquired with a Nikon AXR confocal microscope. Guinea pig anti-TRPV1 (Invitrogen, catalog PA129770, 1:1,000 dilution), rabbit anti-CGRP (Immunostar, catalog 24112, 1:500), donkey anti-mouse NeuN (Cell Signaling Technology, catalog 94403, 1:500 dilution), goat anti-rabbit Cy3 (Jackson Immunoresearch, catalog 111-165-003, 1:2,000 dilution), goat anti-mouse DyLight 405 (Jackson Immunoresearch, catalog 115-475-003, 1:250 dilution), and goat anti-guinea pig Cy3 (Jackson Immunoresearch, catalog 106-165-003, 1:2,000 dilution) were used.
Ding W., Yang L., Chen Q., Hu K., Liu Y., Bao E., Wang C., Mao J, & Shen S. (2023). Foramen lacerum impingement of trigeminal nerve root as a rodent model for trigeminal neuralgia. JCI Insight, 8(11), e168046.
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3

Immunostaining of Aristae in Drosophila

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Aristae, distal portions removed prior to fixation, were stained as
described (Knecht et al., 2017 (link)).
Primary antibodies were: guinea pig anti-IR21a, peptide epitope:
EDEESREALESLQRLDEFMD (1:100, Proteintech, Chicago), rabbit anti-IR25a
(1:100) (Benton et al., 2009 (link)), rabbit
anti-IR93a (1:100)(Knecht et al.,
2016), chicken anti-GFP (1:1000, Abcam 13970), and mouse anti-E7
tubulin beta (1:100, DSHB). Secondary antibodies (used at 1:200) were: goat
anti-chicken Alexa 488 (A-11039, Life Technologies), goat anti-mouse Alexa
488 (A11029, Invitrogen), goat anti-guinea pig Cy3 (Jackson ImmunoResearch),
and goat anti-rabbit Cy3 (Jackson ImmunoResearch). Images were obtained on
Leica Sp5 confocal or Nikon A1 confocal and processed using FIJI and Adobe
Photoshop. For the fluorescence immunostaining in Figure 4A: n=8 samples for wild
type; n=10 for Ir21a mutants, n= 9 for
Ir25a mutants and n=4 for Ir93amutants.
Budelli G., Ni L., Berciu C., van Giesen L., Knecht Z.A., Chang E.C., Kaminski B., Silbering A.F., Samuel A., Klein M., Benton R., Nicastro D, & Garrity P.A. (2019). Ionotropic Receptors Specify the Morphogenesis of Phasic Sensors Controlling Rapid Thermal Preference in Drosophila.. Neuron, 101(4), 738-747.e3.
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