Log In Sign up
The largest database of trusted experimental protocols

Rat monoclonal anti ha

Manufactured by Merck Group
Visit Supplier
Sourced in United States

Rat monoclonal anti-HA is a laboratory reagent used to detect and identify proteins that have been engineered to contain the Hemagglutinin (HA) tag. This antibody specifically binds to the HA tag, allowing for the visualization and quantification of HA-tagged proteins in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Anti iba1, by Fujifilm (2 mentions) Anti neun, by Merck Group (2 mentions) Anti mouse cy5, by Jackson ImmunoResearch (1 mentions) X treme 9, by Roche (1 mentions)

Spelling variants of this product

Anti-HA (475 citations) Rat anti-HA (41 citations)

3 protocols using rat monoclonal anti ha

1

Immunohistochemical analysis of mouse brain

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were anesthetized with Pental (1:2) and were perfused with PBS. Brains and spinal cords were excised and fixed for 4 hours in 2% paraformaldehyde. Brains were incubated for 72 hours in 30% sucrose and spinal cords in 18% EDTA prior to OCT (TissueTek) imbedding and freezing. Post fixed and stained 15-30 µm frozen sections were blocked in 2% horse serum for 2 hours and incubated overnight in 4°C with the primary antibody. Sections were washed 3 times in PBSX1 0.02% TritonX (Sigma) and conjugated with secondary antibody for 1 hour in 4°C. Before covering samples were washed 3 times and incubated for 5 minutes with Hoechst. Sections were analyzed by confocal laser scanning microscope Olympus BX51. Image acquisition was processed by Olympus image browser software. The following antibodies were used: Rat monoclonal anti-HA (1:100, Sigma), Rabbit polyclonal anti-Iba1 (1:250, Wako), mouse polyclonal anti-NeuN (1:100, Millipore) and Hoechst 33342 (1:10,000,Invitrogen).
Haimon Z., Volaski A., Orthgiess J., Boura-Halfon S., Varol D., Shemer A., Yona S., Zuckerman B., David E., Chappell-Maor L., Bechmann I., Gericke M., Ulitsky I, & Jung S. (2018). Re-evaluating Microglia Expression Profiles Using RiboTag and Cell Isolation Strategies. Nature immunology, 19(6), 636-644.
+ Open protocol
+ Expand
2

Immunohistochemical analysis of mouse brain

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were anesthetized with Pental (1:2) and were perfused with PBS. Brains and spinal cords were excised and fixed for 4 hours in 2% paraformaldehyde. Brains were incubated for 72 hours in 30% sucrose and spinal cords in 18% EDTA prior to OCT (TissueTek) imbedding and freezing. Post fixed and stained 15-30 µm frozen sections were blocked in 2% horse serum for 2 hours and incubated overnight in 4°C with the primary antibody. Sections were washed 3 times in PBSX1 0.02% TritonX (Sigma) and conjugated with secondary antibody for 1 hour in 4°C. Before covering samples were washed 3 times and incubated for 5 minutes with Hoechst. Sections were analyzed by confocal laser scanning microscope Olympus BX51. Image acquisition was processed by Olympus image browser software. The following antibodies were used: Rat monoclonal anti-HA (1:100, Sigma), Rabbit polyclonal anti-Iba1 (1:250, Wako), mouse polyclonal anti-NeuN (1:100, Millipore) and Hoechst 33342 (1:10,000,Invitrogen).
Haimon Z., Volaski A., Orthgiess J., Boura-Halfon S., Varol D., Shemer A., Yona S., Zuckerman B., David E., Chappell-Maor L., Bechmann I., Gericke M., Ulitsky I, & Jung S. (2018). Re-evaluating Microglia Expression Profiles Using RiboTag and Cell Isolation Strategies. Nature immunology, 19(6), 636-644.
+ Open protocol
+ Expand
3

Visualizing Endosome Localization of HA-tagged I329L

Check if the same lab product or an alternative is used in the 5 most similar protocols
Vero cells grown on coverslips were transfected using X-treme9 (Roche) with pcDNA3-HA empty vector or pcDNA3-I329L-HA and EEA1 Ct-tomato plasmid (to visualize early endosome marker). At 48 h post-transfection, the cells were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature. The cells were permeabilized with PBS containing 0.1% Triton X-100 for 20 min, washed and blocked with PBS containing 5% normal goat serum and 0.05% Tween 20 for 30 min. In an alternative protocol, the fixation and permeabilization step were omitted in order to stain cell surface proteins. The cells were then incubated with a rat monoclonal anti-HA (SIGMA, St. Louis, MO, USA), and then anti-mouse cy5 (Jackson, Chicago, IL, USA) to visualize the HA tagged I329L, and the cell nuclei were stained with DAPI.
Correia S., Moura P.L., Ventura S., Leitão A, & Parkhouse R.M. (2023). I329L: A Dual Action Viral Antagonist of TLR Activation Encoded by the African Swine Fever Virus (ASFV). Viruses, 15(2), 445.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!