High-purity lecithin of soybean, cholesterol, polyethylene glycol 4000, glucosidase, streptozotocin and quercetin were purchased from Sigma (MO, USA). Rat TNF-α and IL-1β ELISA kits were purchased from Elabscience Biotechnology Co. (Wuhan, China). The rat AGEs ELISA kit was purchased from Cusabio (Wuhan, China). All the other chemicals were purchased from Sigma (MO, USA). The SOD assay kit was purchased from Sigma-Aldrich (Shanghai, China).The MDA and GSH-PX assay kits were purchased from Elabscience Biotechnology Co. (Wuhan, China). All the other reagents used in the present study were of analytical grade and were purchared from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
Glucosidase
Manufactured by Merck Group
Sourced in United States, China
Glucosidase is an enzyme that catalyzes the hydrolysis of glycosidic bonds in carbohydrates, releasing glucose molecules. It plays a crucial role in the breakdown of complex carbohydrates in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Pepsin, by Merck Group (4 mentions)
α amylase, by Merck Group (3 mentions)
Quercetin, by Merck Group (2 mentions)
Xylanase, by Merck Group (2 mentions)
Flashzyme plus 200, by Merck Group (2 mentions)
Bile salt, by Merck Group (2 mentions)
Trypsin, by Merck Group (2 mentions)
Amylase, by Merck Group (2 mentions)
Polyethylene glycol 4000, by Merck Group (1 mentions)
Sod assay kit, by Merck Group (1 mentions)
Rat ages elisa kit, by Cusabio (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Celluclast 1.5 l, by Novozymes (1 mentions)
Celluclast 1.5 l, by Merck Group (1 mentions)
α amylase, by Maclin Biochemical Technology (1 mentions)
Pancreatin, by Merck Group (1 mentions)
β lactoglobulin, by Merck Group (1 mentions)
Methanol, by Sinopharm (1 mentions)
Sodium hypochlorite, by Sinopharm (1 mentions)
15 protocols using glucosidase
1
Lecithin-Based Nanoparticle Formulation
2
Enzymatic Optimization for Biomass Saccharification
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In order to select the enzyme complex for the SSF process, tests were performed using selected enzymes—Flashzyme Plus 200 and Celluclast 1.5 L (Novozymes, Bagsværd, Denmark) and their supplementation with glucosidase 20 CBU·g−1 and xylanase 500 XU·g−1 (Sigma-Aldrich, Darmstadt, Germany).
The composition of Flashzyme Plus 200 (90 FPU·mL−1, 2430 XU·mL−1) is endoglucanase, cellobiohydrolase, cellobiase, xylanase and mannanase, and Celluclast 1.5 L (62 FPU·mL−1, 278 XU·mL−1) consists of cellulase from Trichoderma reesei.
Enzymatic tests were carried out for 5% of biomass with the enzyme in the amount of 10 FPU·g−1, at pH 4.8 and for 24 h at 38 °C. The selection criterion was the content of reducing sugars determined using the Miller’s method.
The composition of Flashzyme Plus 200 (90 FPU·mL−1, 2430 XU·mL−1) is endoglucanase, cellobiohydrolase, cellobiase, xylanase and mannanase, and Celluclast 1.5 L (62 FPU·mL−1, 278 XU·mL−1) consists of cellulase from Trichoderma reesei.
Enzymatic tests were carried out for 5% of biomass with the enzyme in the amount of 10 FPU·g−1, at pH 4.8 and for 24 h at 38 °C. The selection criterion was the content of reducing sugars determined using the Miller’s method.
3
Barley Powder Preparation and Analysis
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Yangzhou's No. 3 Barley Powder (BP) was purchased from Yancheng Shuangzeng Agrochemical Technology Co. LTD (Yancheng, China). Extruded Barley Powder (EBP, 40 Hz of screw rotation, 30 Hz of feeding speed, 140°C of sleeve temperature, and 30% of raw water content) and fermented barley powder (FBP, fermentation with Lactobacillus plantarum dy-1) were prepared in our laboratory.
α-amylase, glucosidase, pepsin, trypsin, and bile salt were purchased from Sigma Company (St. Louis, MO, United States) as biological reagents. Gallic acid, catechin, epicatechin, chlorogenic acid, ferulic acid, caffeic acid, 2-hydroxybenzoic acid, p-coumaric acid, vanillin acid, and 3, 4-dihydroxybenzoic acid were purchased from Tixi Ai (Shanghai) Chemical Industrial Development Co, Ltd.
α-amylase, glucosidase, pepsin, trypsin, and bile salt were purchased from Sigma Company (St. Louis, MO, United States) as biological reagents. Gallic acid, catechin, epicatechin, chlorogenic acid, ferulic acid, caffeic acid, 2-hydroxybenzoic acid, p-coumaric acid, vanillin acid, and 3, 4-dihydroxybenzoic acid were purchased from Tixi Ai (Shanghai) Chemical Industrial Development Co, Ltd.
4
Oxidized Corn Starch Synthesis and Characterization
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Oxidized corn starch was synthesized according to the literature methods with slight modifications [17 (link)]. See Table S1 and Figure S1 for more structural, detailed information. β-lactoglobulin, pancreatin, glucosidase, and pepsin were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Astaxanthin, bile salt, and α-amylase were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Medium-chain triglycerides (MCT) were purchased from Shanghai Source Leaf Biological Technology Co., Ltd. Sodium hypochlorite, methanol, and all other analytical grade reagents were purchased from Sinopharm Chemical Reagent Company (Shanghai, China). Microfluidic chip (68 mm × 95 mm × 4 mm, liquid holdup: 0.51 mL) and two-channel syringe pump system were purchased from Suzhou Wenhao Microfluidic Technology Co., Ltd., Suzhou, China.
5
Enzymatic Characterization for SHF and SSF
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The enzymes cellulolytic activity was determined according to NREL LAP Measurement of Cellulase Activities. In turn, enzymes xylanolytic activity was determined according to the Osaka University procedure (with changes) [41 ].
In order to select the enzyme complex for SHF and SSF processes, tests were performed using selected enzymes (Flashzyme Plus 200:Celluclast 1.5L) and their supplementation with glucosidase 20 CBU·g−1 of solid and xylanase 500 XU·g−1 of solid (Sigma-Aldrich, Darmstadt, Germany ). Enzymatic tests were carried out for 5% of biomass with the enzyme in the amount of 10 FPU·g−1 of solid, at a pH of 4.8, and during 24 h at 55 °C for the SHF process and at 38 °C for the SSF process. The selection criterion was the content of reducing sugars determined by the Miller’s method.
In order to select the enzyme complex for SHF and SSF processes, tests were performed using selected enzymes (Flashzyme Plus 200:Celluclast 1.5L) and their supplementation with glucosidase 20 CBU·g−1 of solid and xylanase 500 XU·g−1 of solid (Sigma-Aldrich, Darmstadt, Germany ). Enzymatic tests were carried out for 5% of biomass with the enzyme in the amount of 10 FPU·g−1 of solid, at a pH of 4.8, and during 24 h at 55 °C for the SHF process and at 38 °C for the SSF process. The selection criterion was the content of reducing sugars determined by the Miller’s method.
6
Sorghum and Oat-based Fermented Product
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Sorghums and oats were obtained from the Shanxi breeding Institute (Shanxi, China). The OS and SS were prepared in the laboratory with a purity of 91.51 ± 1.45%, and 92.13 ± 1.23% (AACC 76.13), respectively. Thermostable α-amylase from Bacillus subtilis was purchased from Shanghai Yuan ye Bio-Technology Co., Ltd. (Shanghai, China), Pepsin and glucosidase were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Lactiplantibacillus plantarum (Lactobacillus plantarum subsp. Plantarum, GDMCC 1.1797), Lactobacillus acidophilus (GDMCC 1.321) and Lactobacillus delbrueckii (Lactobacillus delbrueckii subsp. Bulgaricus, GDMCC 1.155) were purchased from Guangdong Microbial Culture Collection Center (Guangdong, China). All the other chemicals were of analytical grade.
7
Gellan Gum-Based Encapsulation for Bioactive Delivery
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A commercial high acyl gellan gum, Kelcogel LT100 (CPKelco, Atlanta, GA, USA; Lot# 9L0058A CAS No:7101-52-1) was used in this study as well as medium-chain triglycerides (MCT, food-grade, Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China), derived from palm kernel and containing fatty acids that have a chain length of 8–12 carbon atoms; β-carotene (purity > 96%, food-grade, Zhejiang Xinchang Pharmaceutical Co., Ltd., Hangzhou, China); n-hexane, chloroform, sodium chloride, sodium hydroxide, hydrochloric acid, ethanol, potassium chloride, sodium dihydrogen phosphate, sodium sulfate, calcium chloride (analytical purity, Sinopharm Group Chemical Reagent Co., Ltd., Shanghai, China), α-amylase, pepsin, trypsin, glucosidase, bile salt, and Nile red dye (Sigma-Aldrich, Inc., St. Louis, MO, USA).
8
Antioxidant and Enzyme Inhibition Assays
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Folin-Ciocalteu, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), nitrophenyl--D-glucopyranoside, -glucosidase, and gallic acid were obtained from Sigma-Aldrich (MO, USA). PiSA Farmacéutica (Jalisco, Mexico) kindly donated the acarbose needed for this work.
9
Analytical Techniques for Bioactive Compounds
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HPLC-grade acetonitrile, water, methanol, acetone, acetic acid, ethanol, hexane, ethyl acetate, diethyl ether, hydrochloric acid, sulphuric acid, ammonium sulphate, and boric acid were purchased from Merck KGaA (Darmstadt, Germany). Hydroxide sodium was from Fluka (Buchs, Switzerland). Ferulic acid, catechin, quercetin, and rutin (Sigma-Aldrich, St. Louis, MO, USA) were used for the calibration curves. Glucosidase, amyloGlucosidase, peroxidase, and α-amylase were purchased from Sigma-Aldrich (St. Louis, MO, USA).
10
Glucosidase Inhibition Assay Protocol
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The assay was performance in 96-well microtiter plate as previously described with slight modifications 20 (link) . Each well contained 50 µL of solution of sample diluted in sodium phosphate buffer 100 mM (pH 6.8, 5% DMSO) and 40 µL solution of "-glucosidase (Sigma) 0.2 U mLG 1 , the mixture was incubated at room temperature for 20 min. Then, the mixture was added 40 µL of p-NPG 5 mM as a substrate and incubated for 20 min. Finally, 130 µL of Na 2 CO 3 0.2 M was placed in the well to stop the reaction before measuring OD 405 nm by Perkin Elmer 2030 Elisa reader. The reaction mixture not added enzyme is blank. Acarbose was used as a positive control. Buffer used for dissolving the extracts is negative control. Each sample was tested with 3 replicates. The percentage of inhibition activity (%I) was calculated according to the equation:
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