Vectors of miR-152 mimics, miR-152 inhibitor and miR-shNC were purchased from GenePharma Company in China. The sequences of ACAA2 and HSD17B12 were amplified with PCR instrument with Not I and Xho I restriction sites engineered at primer ends. Amplified DNA fragments were cloned into pmiR-RB-REPORT vector to construct recombinant vectors pmiR-RB-REPORT- ACAA2-mut/WT/si and pmiR-RB-REPORT- HSD17B12-mut/WT/si. To investigate the association between target genes with apoptosis, cell proliferation and triglyceride production, PBI-CMV3-ACAA2/HSD17B12 and sh234-ACAA2-181/sh234-HSD17B12-474 plasmids were respectively constructed, and then transfected into cells using lipofectamine TM 2000 according to the manufacturer’s instructions.
Mir 152 inhibitor
Manufactured by GenePharma
Sourced in China
The MiR-152 inhibitor is a laboratory reagent designed to suppress the activity of the microRNA miR-152. This inhibitor can be used to study the biological functions and regulatory mechanisms of miR-152 in various cellular and molecular research applications.
Automatically generated - may contain errors
Lab products found in correlation
Mir 152 mimic, by GenePharma (3 mentions)
Carbon tetrachloride ccl4, by Merck Group (2 mentions)
Curcumin cur, by Merck Group (1 mentions)
5 aza 2 deoxycytidine 5 aza, by Merck Group (1 mentions)
Si pvt1, by GenePharma (1 mentions)
Si pvt1 2, by GenePharma (1 mentions)
Desmin, by Abcam (1 mentions)
α sma, by Abcam (1 mentions)
Vimentin, by Abcam (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
E cadherin, by Abcam (1 mentions)
Hiperfect transfection reagent, by Qiagen (1 mentions)
5 protocols using mir 152 inhibitor
1
Investigating microRNA Regulation of Lipid Metabolism Genes
2
Epigenetic Regulation of miR-152 in Liver
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MiRNA-negative control (miR-NC), a miR-152 inhibitor, and a miR-152 mimic were bought from GenePharma (Shanghai, China). Carbon tetrachloride (CCl4) and 5-Aza-2′-deoxycytidine (5-Aza) as well as Curcumin (Cur) were obtained from Sigma (St Louis, MO, USA) and Rg1 (≥98% purity) was obtained from Abcam (Cambridge, MA, USA). Adenoviral vectors containing DNA methyltransferase 1 (Ad-DNMT1) or a contrastive scrambled sequence were obtained from GenePharma Biotechnology.
3
RNA Interference Experiments in HSCs
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RNA interference experiments were performed following the manufacturer's protocols. siPVT1 (sense, 5′-AAGGAAGCUCUUCUUGAGC-3′; antisense, 5′-GCUC AAGAAGAGCUUCCUU-3′), siPVT1-2 (sense, 5′-GGAAUGCUAAGUUCGUAGC UU-3′; antisense, 5′-AAGCUACGAACUUAGCAUUCC-3′) and scrambled siRNA (negative control) were designed and synthesized by GenePharma. miR-152 mimics and miR-152 inhibitor were additionally synthesized by GenePharma. Moreover, siCtrl and miR-NC were used as the negative control. HSCs were transfected with siPVT1, siPVT1-2, miR-152 mimics or miR-152 inhibitor using Lipofectamine RNAiMAX at a final concentration of 10 nM.
4
Sal B Induces Epigenetic Regulation
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Sal B (purity 99%) was extracted and identified by the Chinese National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Its molecular weight is 718, and its molecular formula is C36H30O16. Carbon tetrachloride (CCl4) was obtained from Sigma-Aldrich (St Louis, MO, USA). Antibodies against DNMT3a, DNMT3b, type I collagen, α-SMA, E-cadherin, Vimentin, Desmin, PTCH1, Smo and Gli2 were obtained from Abcam (Cambridge, MA, USA). Antibodies targeting DNMT1 and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Chemically synthesized RNAs, including negative control (miR-NC), miR-152 mimics and miR-152 inhibitor, were obtained from GenePharma (Shanghai, China). For transfection, the cells were transfected with 1 μg of the chemically synthesized RNA.
5
Investigating miR-152 Regulation in Insulin-Producing Cells
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The miR-152 mimic, miR-152 inhibitor, and miRNA mimic and inhibitor controls, were purchased from Shanghai GenePharma Co., Ltd. (Shanghai, China). The miR-152 mimic, GCA GTC TCA GTG CAT GAC AGA, miR-152 inhibitor, CCA UCU UUA CCA GAC AGU GUU A, inhibitor controls CAG UAC UUU UGU GUA GUA C. HiPerFect transfection reagent (Qiagen GmbH, Hilden, Germany) was used for the transfection of miR-152 mimic and inhibitors, and controls. MIN6/INS-1 cells were cultured by plating 6x105 cells into 100-mm Petri dishes. A total of 250 pmol miR-152 mimic or inhibitor, or respective controls were transfected into cells. At 48 h following transfection, the expression of miR-152 and target genes were detected reverse transfection-quantitative polymerase chain reaction (RT-qPCR). Insulin (1 nmol/l) was purchased from United States Biological (Salem, MA, USA).
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