Detachabead
DETACHaBEAD is a magnetic bead-based cell separation product used for the isolation and purification of specific cell types from complex biological samples. It enables the effective separation and recovery of target cells without the need for complex instrumentation.
Lab products found in correlation
19 protocols using detachabead
Isolation of CD19+ B cells from healthy human donors
Isolation of High-Purity CD8+ T Cells
Isolation of Human Blood Cells
Isolation of CD19+ B-cells from Buffy Coats
Isolation of CD19+ B-cells from Buffy Coats
CD19+ B Cell Isolation and EBV Transformation
B cells were infected with EBV to transform lymphocytes. 10 ml of B cells were transferred into a T75 flask. 1.5 ml of stock EBV collected from a B95-8 strain-containing marmoset cell line and 1 ml of phytohemagglutinin P (PHA-P) were added to flask and incubated in 37°C with a 5% CO2 atmosphere incubator. Every 5–7 d, 10 ml of cell culture medium was added. Cells were let to grow in the CO2 atmosphere incubator for 30 d until all B cells were transformed to LCLs.
Culturing B-cell and Monocytic Lines
Primary tonsillar B cells from two seronegative individuals of the study cohort were isolated with anti-CD19 magnetic beads from frozen total cell suspensions of mechanically homogenized preparations and released with DETACHaBEAD as instructed by manufacturer (Invitrogen).
Isolation and Activation of B Cells
Induction of colitis via T-cell transfer
B cell immortalization from apheresis
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