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Fitc conjugated cd71 okt9

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FITC-conjugated CD71 (OKT9) is a fluorescently labeled monoclonal antibody that binds to the transferrin receptor (CD71) on the surface of cells. The FITC (Fluorescein Isothiocyanate) fluorescent label allows for the detection and analysis of CD71-expressing cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Annexin 5 bv421, by BD (2 mentions) Fortessa flow cytometer, by BD (2 mentions) Sytox blue, by Thermo Fisher Scientific (2 mentions) Apc conjugated cd49d 9f10, by BioLegend (2 mentions) Sytox orange, by Thermo Fisher Scientific (2 mentions) Annexin 5 apc staining kit, by BD (2 mentions) Apc conjugated glycophorin a cd235a hir2, by Thermo Fisher Scientific (2 mentions) Pe conjugated cd41a hip8, by Thermo Fisher Scientific (2 mentions) Pe conjugated cd11b icrf44, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by BD (2 mentions)

4 protocols using fitc conjugated cd71 okt9

1

Erythroid Cell Phenotyping by Flow Cytometry

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For flow cytometry analysis, in vitro cultured erythroid cells were washed in FACS buffer (1% FBS in PBS) before antibody staining. The following antibodies at indicated dilutions were used in this study: 1:50 APC-conjugated CD235a (Glycophorin A, clone HIR2, 50–153-69, eBioscience), 1:50 FITC-conjugated CD71 (OKT9, 14–0719-82, eBioscience), 1:50 APC-conjugated CD49d (9F10, Biolegend, 304308) and 1:200 FITC-conjugated BAND3 (An et al., 2014 (link); Hu et al., 2013 (link)). For live/ dead cell discrimination Sytox Blue was used according to the manufacturer’s instructions (Thermo Fisher, S34857). For apoptosis analysis, BV421-Annexin V (BD Bioscience, 563973) was used in combination with Sytox Orange according to the manufacturer’s instructions (Thermo Fisher, S34861). FACS analysis was conducted on a BD Bioscience Fortessa flow cytometer at the Whitehead Institute Flow Cytometry core. Data were analyzed using FlowJo software v10.4.2.
Ludwig L.S., Lareau C.A., Bao E.L., Nandakumar S.K., Muus C., Ulirsch J.C., Chowdhary K., Buenrostro J.D., Mohandas N., An X., Aryee M.J., Regev A, & Sankaran V.G. (2019). Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis. Cell reports, 27(11), 3228-3240.e7.
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2

Multicolor Flow Cytometry of Erythroid Cells

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For flow cytometry analysis, in vitro cultured erythroid cells were washed in PBS and stained with propidium iodide (PI), 1:60 APC-conjugated Glycophorin A/CD235a (HIR2, 17-9987-42, eBioscience), 1:60 FITC-conjugated CD71 (OKT9, 11-0719-42, eBioscience), 1:60 PE-conjugated CD41a (HIP8, 12-0419-42, eBioscience) and 1:60 PE-conjugated CD11b (ICRF44, 12-0118-42, eBioscience). For apoptosis analysis the Annexin V-APC staining kit was used according to the manufacturer’s instructions (550474, BD Pharmingen). FACS analysis was conducted on a BD Bioscience LSR II flow cytometer. Data were analyzed using FlowJo 8.6.9 (TreeStar).
Ludwig L.S., Gazda H.T., Eng J.C., Eichhorn S.W., Thiru P., Ghazvinian R., George T.I., Gotlib J.R., Beggs A.H., Sieff C.A., Lodish H.F., Lander E.S, & Sankaran V.G. (2014). Altered translation of GATA1 in Diamond-Blackfan anemia. Nature medicine, 20(7), 748-753.
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3

Erythroid Cell Phenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry analysis, in vitro cultured erythroid cells were washed in FACS buffer (1% FBS in PBS) before antibody staining. The following antibodies at indicated dilutions were used in this study: 1:50 APC-conjugated CD235a (Glycophorin A, clone HIR2, 50–153-69, eBioscience), 1:50 FITC-conjugated CD71 (OKT9, 14–0719-82, eBioscience), 1:50 APC-conjugated CD49d (9F10, Biolegend, 304308) and 1:200 FITC-conjugated BAND3 (An et al., 2014 (link); Hu et al., 2013 (link)). For live/ dead cell discrimination Sytox Blue was used according to the manufacturer’s instructions (Thermo Fisher, S34857). For apoptosis analysis, BV421-Annexin V (BD Bioscience, 563973) was used in combination with Sytox Orange according to the manufacturer’s instructions (Thermo Fisher, S34861). FACS analysis was conducted on a BD Bioscience Fortessa flow cytometer at the Whitehead Institute Flow Cytometry core. Data were analyzed using FlowJo software v10.4.2.
Ludwig L.S., Lareau C.A., Bao E.L., Nandakumar S.K., Muus C., Ulirsch J.C., Chowdhary K., Buenrostro J.D., Mohandas N., An X., Aryee M.J., Regev A, & Sankaran V.G. (2019). Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis. Cell reports, 27(11), 3228-3240.e7.
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4

Multicolor Flow Cytometry of Erythroid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry analysis, in vitro cultured erythroid cells were washed in PBS and stained with propidium iodide (PI), 1:60 APC-conjugated Glycophorin A/CD235a (HIR2, 17-9987-42, eBioscience), 1:60 FITC-conjugated CD71 (OKT9, 11-0719-42, eBioscience), 1:60 PE-conjugated CD41a (HIP8, 12-0419-42, eBioscience) and 1:60 PE-conjugated CD11b (ICRF44, 12-0118-42, eBioscience). For apoptosis analysis the Annexin V-APC staining kit was used according to the manufacturer’s instructions (550474, BD Pharmingen). FACS analysis was conducted on a BD Bioscience LSR II flow cytometer. Data were analyzed using FlowJo 8.6.9 (TreeStar).
Ludwig L.S., Gazda H.T., Eng J.C., Eichhorn S.W., Thiru P., Ghazvinian R., George T.I., Gotlib J.R., Beggs A.H., Sieff C.A., Lodish H.F., Lander E.S, & Sankaran V.G. (2014). Altered translation of GATA1 in Diamond-Blackfan anemia. Nature medicine, 20(7), 748-753.
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