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D amphetamine

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D-amphetamine is a chemical compound used in various laboratory applications. It serves as a stimulant and is commonly employed in research and analytical processes. The core function of D-amphetamine is to facilitate specific experimental procedures and analyses, as determined by the research objectives.

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Lab products found in correlation

Gbr12909 dihydrochloride, by Bio-Techne (2 mentions) Atomoxetine, by Bio-Techne (2 mentions) Hydroxypropyl beta cyclodextrin, by Thermo Fisher Scientific (2 mentions) Paris kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

D-amphetamine sulfate Amphetamine

6 protocols using d amphetamine

1

Selective Neuromodulator Effects on Mouse Behavior

Cited 2 times
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In all experiments mice were administered the selective COMT inhibitor tolcapone (30mg/kg; TRC Inc)(25 (link),41 (link)) and/or the selective DAT blocker GBR-12909 dihydrochloride (6mg/kg, Tocris)(42 (link)). These doses are effective at altering behavior without causing motor stereotypies (see Supplementary Information). D-amphetamine (in sulfate formulation; Tocris) was used at 4mg/kg (43 (link)), and the NET blocker atomoxetine (in hydrochloride formulation; Tocris) at 1mg/kg (44 (link)) in the voltammetry experiment only. All drugs were dissolved in 20% hydroxypropyl-beta-cyclodextrin (Acros Organics) in 0.9% saline (AquPharm), which served as a vehicle control in all experiments. All drugs were delivered by intraperitoneal injection.
Korn C., Akam T., Jensen K.H., Vagnoni C., Huber A., Tunbridge E.M, & Walton M.E. (2021). Distinct roles for dopamine clearance mechanisms in regulating behavioral flexibility. Molecular Psychiatry, 26(12), 7188-7199.
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2

Selective Neuromodulator Effects on Mouse Behavior

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
In all experiments mice were administered the selective COMT inhibitor tolcapone (30mg/kg; TRC Inc)(25 (link),41 (link)) and/or the selective DAT blocker GBR-12909 dihydrochloride (6mg/kg, Tocris)(42 (link)). These doses are effective at altering behavior without causing motor stereotypies (see Supplementary Information). D-amphetamine (in sulfate formulation; Tocris) was used at 4mg/kg (43 (link)), and the NET blocker atomoxetine (in hydrochloride formulation; Tocris) at 1mg/kg (44 (link)) in the voltammetry experiment only. All drugs were dissolved in 20% hydroxypropyl-beta-cyclodextrin (Acros Organics) in 0.9% saline (AquPharm), which served as a vehicle control in all experiments. All drugs were delivered by intraperitoneal injection.
Korn C., Akam T., Jensen K.H., Vagnoni C., Huber A., Tunbridge E.M, & Walton M.E. (2021). Distinct roles for dopamine clearance mechanisms in regulating behavioral flexibility. Molecular Psychiatry, 26(12), 7188-7199.
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3

Acute D-Amphetamine Effects in Mice

Cited 1 time
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D-Amphetamine (2813/100, Tocris Bioscience, dissolved in saline, 2 mg/kg) or saline was injected i.p. into the mice 13 (link), 21 (link), and the mice were assessed directly after D-Amphetamine injection.
Yu X., Ba W., Zhao G., Ma Y., Harding E.C., Yin L., Wang D., Li H., Zhang P., Shi Y., Yustos R., Vyssotski A.L., Dong H., Franks N.P, & Wisden W. (2020). Dysfunction of ventral tegmental area GABA neurons causes mania-like behavior. Molecular Psychiatry, 26(9), 5213-5228.
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4

Amphetamine-induced gene expression

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Tissue for RNA analysis was obtained following cervical dislocation and isolation of the brain. To assess sensitivity to amphetamine an intraperitoneal injection of saline or 0.5 mg/kg D‐amphetamine (Tocris Bioscience, Bristol, UK) was administered. Brains were isolated 60 min following injection by cervical dislocation and ventral striatum was isolated. Embryonic RNA was extracted using PARIS kit (Life Technologies, UK). Adult RNA was extracted using standard phenol extraction methods and DNase treated using RNAse‐free DNase treatment and removal kit (Applied Biosystems, UK) according to the manufacturer's instructions. Expression was normalized (ΔCt) to the geometric mean Ct value of βactin and Hprt as these genes have previously been verified in‐house. Statistical analysis was carried out on transformed ΔCt values.
McNamara G.I., Davis B.A., Browne M., Humby T., Dalley J.W., Xia J., John R.M, & Isles A.R. (2017). Dopaminergic and behavioural changes in a loss‐of‐imprinting model of Cdkn1c. Genes, Brain, and Behavior, 17(2), 149-157.
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5

Intraperitoneal Drug Administration

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d-Amphetamine (Tocris Bioscience, Bristol, UK), PCP (Sigma-Aldrich, St. Louis, MO), lorcaserin (HCl salt, Carbosynth, San Diego CA), and compound (+)-19 were dissolved in physiological saline and administered by intraperitoneal injection (IP) at a concentration of 10 mL/kg.
Zhang G., Cheng J., McCorvy J.D., Lorello P.J., Caldarone B.J., Roth B.L, & Kozikowski A.P. (2017). Discovery of N-Substituted 2-Phenylcyclopropylmethylamines as Functionally Selective Serotonin 2C (5-HT2C) Receptor Agonists for Potential Use as Antipsychotic Medications. Journal of medicinal chemistry, 60(14), 6273-6288.
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6

Amphetamine-induced Behavioral Assessment

Cited 1 time
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D-Amphetamine (2813/100, Tocris Bioscience, dissolved in saline, 2 mg/kg) or saline was injected i.p. into the mice [13 (link), 21 (link)], and the mice were assessed directly after D-Amphetamine injection.
Yu X., Ba W., Zhao G., Ma Y., Harding E.C., Yin L., Wang D., Li H., Zhang P., Shi Y., Yustos R., Vyssotski A.L., Dong H., Franks N.P, & Wisden W. (2020). Dysfunction of ventral tegmental area GABA neurons causes mania-like behavior. Molecular Psychiatry, 26(9), 5213-5228.
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