Advia chemistry xpt

Blood samples were collected from the participants after an 8-h fast. Hematological analysis of the level of serum glycated albumin (GA), lactate dehydrogenase (LDH), glucose AC (fasting blood glucose), glycosylated hemoglobin (HbA1c), connecting peptide (C-peptide), insulin antibodies (insulin Ab), triiodothyronine (T3), free thyroxine (FT4), and thyroid-stimulating hormone (TSH) was conducted. The hemoglobin level was determined using an automatic analyzer (XN-9000, Sysmex, Japan). The serum GA and LDH levels were analyzed by enzymatic methods by an automatic analyzer (ADVIA Chemistry XPT, Siemens, Germany), and shaking should be avoided while drawing blood. Glucose AC level was analyzed using the hexokinase method, and HbA1c level was determined through high-performance liquid chromatography using automatic analyzers (ADVIA Chemistry XPT and Variant II Turbo 2.0 System, Bio-Rad, USA). C-peptide level was detected by the method of chemiluminescence enzyme immunoassay using an automatic analyzer (ADVIA Chemistry XPT, Siemens, Germany). Serum insulin Ab level was measured by immunoradiometric binding assay in an automatic analyzer (PerkinElmer Cisbio, USA). The thyroid function profile (including T3, FT4, and TSH) was measured by the radioimmunoassay technique using an automatic analyzer (Atellica IM 1300 analyzer, Siemens, Germany).

Leucocytes and differential counts were analysed per routine. High sensitivity CRP was analysed by an immunoturbidimetric method using the MODULAR platform (Roche Diagnostics, Basel, Switzerland) and Siemens Advia Chemistry XPT (Siemens Healthcare, Erlangen, Germany). High sensitivity TnT was analysed by electrochemiluminescence immunoassay using Roche Elecsys 2010 and Roche Cobas 8000 (Roche Diagnostics, Basel, Switzerland).

Cryopreserved peripheral blood mononuclear cells (PBMCs) were provided by the Spanish HIV Hospital Universitario Gregorio Marañón BioBank (HIV HUGM BioBank) [22 (link)]. Thirty‐millilitre samples of fresh whole blood from HD volunteers were collected in ethylene diamine tetra‐acetic acid tubes. The percentage of CD4+ and CD8+ T cells was determined in fresh whole blood using Cytomics FC (Beckman‐Coulter, Brea, CA, USA). Plasma and PBMCs were immediately isolated by Ficoll‐Paque density gradient centrifugation and stored at −20°C and −170°C, respectively, until subsequent analysis of IL‐10 and TNF‐α by ELISA assay (R&D Systems, Minneapolis, MN), high‐sensitivity CRP (hsCRP) (ADVIA Chemistry XPT, Siemens Healthineers) and IL‐6 (COBAS e411, Roche Molecular Systems, Basel, Switzerland). Plasma HIV‐1 RNA concentration was measured using quantitative polymerase chain reaction (COBAS Ampliprep/COBAS Taqman HIV‐1 test, Roche Molecular Systems) according to the provided manufacturer's protocol.

Blood samples were meticulously collected between 8 and 9 a.m. following an overnight fast, a precautionary measure aimed at minimizing potential confounding factors and ensuring the precise assessment of bone health. Utilizing enzymatic methods (ADVIA Chemistry XPT; SIEMENS, German), we conducted a thorough analysis, encompassing the measurement of serum uric acid, creatinine, phosphorus, calcium, and the serum lipid profile, which included total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglyceride levels, as well as fasting blood glucose. The estimated glomerular filtration rate (eGFR) was calculated using the CKD-EPI formula (mL/min). For the evaluation of markers of bone turnover, serum was separated by centrifugation and kept at − 20 °C for the determination of parathyroid hormone, c-terminal telopeptide, total osteocalcin, intact N-terminal propeptide of type I collagen, 25-hydroxyvitamin D, sclerostin and osteocalcin using electrochemiluminescence assays (Cobas e601; Roche Diagnostics, Basel, Switzerland). Additionally, plasma glycated hemoglobin levels were determined with high-performance liquid chromatography (MQ-6000; Shanghai Medconn Biotechnology Co., Ltd., China).

Clinical data from the patients were collected twice: at pre- and post-Cana treatment. Meanwhile, data from the healthy controls were obtained at baseline. Demographic and health-related data such as name, gender, age, medication history, surgical history, history of contact lens use, past medical conditions, and dietary habits were collected using comprehensive questionnaires. Height and weight measurements were taken in the morning on an empty stomach, and all participants wore uniform clothing. FPG concentrations were measured using an Automatic Biochemical Analyzer (TBA-120 FR, Toshiba, Japan). Glycated serum protein (GSP) levels were measured using an automatic biochemical analyzer (ADVIA Chemistry XPT, Siemens, USA). Plasma HbAlc concentrations were measured using high-performance liquid chromatography (Bio-Rad D-10, Bio-Rad Laboratories Co., Ltd., Germany). Routine blood tests were performed using the Swelab Alfa Cell analyzer (Boule Diagnostics, AB, Sweden). Urinary microalbumin (UMALB) and creatinine (UCR) levels were assessed using a DCA Vantage Analyzer (Siemens, Chapel Lane Swords Co., Dublin, Ireland). Triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) levels were measured using an automatic biochemical analyzer (Abbott C1600, Illinois, USA).