Elav gal4c155

Manufactured by Bloomington Drosophila Stock Center

Sourced in United States

The Elav-GAL4C155 is a genetic tool used in Drosophila research. It is a transgenic fly line that expresses the yeast transcriptional activator GAL4 under the control of the elav (embryonic lethal, abnormal vision) promoter, which drives expression in all post-mitotic neurons. This allows for targeted gene expression or manipulation in the neurons of Drosophila.

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8 protocols using elav gal4c155

Drosophila Genetics for Neurodegeneration

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GAL4/UAS fly crosses were grown on a cornmeal-agar diet at 29 °C from the egg-laying to the adult stage. dDOR mutant and UAS-dDOR-F (“FENLL”) and UAS-dDOR-L (“Long”) lines were from A.A. Teleman^{32 (link)}, elav-GAL4^C155 (neuronal driver), mef2-GAL4 (muscle driver), CG-GAL4 (fat body driver), UAS-mCherry::Atg8a, UAS-GFP::Atg8a, dPINK1^B9, UAS-SNCA^A30P, UAS-dParkin_IR (TRiP line HMS01800), UAS-dPINK1_IR (TRiP line JF01203), UAS-Dicer2 and UAS-mCD8::GFP were from the Bloomington Drosophila Stock Center (BDSC), UAS-mtKeima was from M.J. Clague and A.J. Whitworth^{33 (link)}, and TH-GAL4 was from S. Birman. UAS-mit::dendra2 line stains mitochondrial matrix and is described in^{34 (link)}. UAS-dDOR::GFP line (C-terminal tag) was generated using the pUASp-GFP-W gateway vector from T. Murphy and inserted in the fly genome by random P-element transgenesis.

Dinh E., Rival T., Carrier A., Asfogo N., Corti O., Melon C., Salin P., Lortet S, & Kerkerian-Le Goff L. (2021). TP53INP1 exerts neuroprotection under ageing and Parkinson’s disease-related stress condition. Cell Death & Disease, 12(5), 460.

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Drosophila Alzheimer's Disease Model

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All fly lines were raised under standard conditions at 20–25°C on standard yeasted Drosophila media (Applied Scientific Jazz Mix Drosophila Food; Thermo Fisher Scientific). The pan-neuronal driver elav-Gal4^C155 (#458) and the transgenic UAS line carrying human Aβ₄₂ (#33769) were purchased from Bloomington Drosophila Stock Center. We generated stocks carrying UAS-Tip60 (UAS-dTip60^WT C) previously (Lorbeck et al. 2011 (link)). The transgenic UAS-Tip60 fly line was crossed into the UAS-Aβ₄₂ background using standard genetic techniques, as previously described (Pirooznia et al. 2012 (link)), to generate the UAS-Aβ₄₂;UAS-Tip60 double transgenic fly line. The w¹¹¹⁸ line (Bloomington Drosophila Stock Center, #3605) served as the genetic background control. All experimental crosses were performed at an average physiological temperature of 20–25°C.

Zhang H., Karisetty B.C., Bhatnagar A., Armour E.M., Beaver M., Roach T.V., Mortazavi S., Mandloi S, & Elefant F. (2020). Tip60 protects against amyloid-β-induced transcriptomic alterations via different modes of action in early versus late stages of neurodegeneration. Molecular and cellular neurosciences, 109, 103570.

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Drosophila Genetics for Neuroprotective Pathways

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Flies were raised on standard maize media at 25°C at a 12-h LD cycle. The elav-GAL4[C155] driver was obtained from the Bloomington Stock Center (Indiana, US). The UAS-RNAi lines [Keap1 (CG3962) and CncC (CG43286)] were purchased from the Vienna Drosophila Resource Centre (VDRC). The UAS-CncC line was kindly provided by Dirk Bohmann, University of Rochester, USA (Sykiotis and Bohmann, 2008 (link); Pitoniak and Bohmann, 2015 (link)). The UAS/GAL4 bipartite expression system was utilized to drive pan-neuronal expression. The elav-GAL4 driver (female flies) and the UAS responder lines (male flies) were crossed to obtain offspring expressing the genes of interest. As a control for the RNAi strains, a line carrying an empty RNAi vector inserted in the AttP40 site was used and crossed to the elav-GAL4 driver (referred to as RNAi Ctrl). For CncC overexpressing (OE) lines, experimental lines were compared to control obtained by crossing the GAL4 (elav Ctrl) and UAS lines to w¹¹¹⁸. The homozygote w¹¹¹⁸ line was used as a control in the pharmacology experiments.

Spiers J.G., Breda C., Robinson S., Giorgini F, & Steinert J.R. (2019). Drosophila Nrf2/Keap1 Mediated Redox Signaling Supports Synaptic Function and Longevity and Impacts on Circadian Activity. Frontiers in Molecular Neuroscience, 12, 86.

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Drosophila Neuronal Phenotype Assay

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Flies were maintained on standard cornmeal medium at 25 °C. para^bss were gifts from Dr. Kevin O’Dell (University of Glasgow). The elav-GAL4^C155 (stock no. 458) was obtained from Bloomington and UAS-RNAi lines were obtained from the Vienna Drosophila Resource Center. Para^bss;GAL4^Cha(19B)was derived by crossing para^bss with GAL4^Cha(19B) (a gift from Dr. Paul Salvaterra, City of Hope, USA).

Lin W.H., He M., Fan Y.N, & Baines R.A. (2018). An RNAi-mediated screen identifies novel targets for next-generation antiepileptic drugs based on increased expression of the homeostatic regulator pumilio. Journal of Neurogenetics, 32(2), 106-117.

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Drosophila Genetics Protocols for RNAi

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Flies were raised at 25 °C in LD12:12 on standard maize food. The elav-GAL4 (c155) and w; UASeGFP; + (5431) lines were obtained from the Bloomington Stock Center (Bloomington, Indiana). The w; +; UASHTT93Q and w; +; UASHTT20Q transgenic line was kindly provided by J. Lawrence Marsh and Leslie Thompson (University of California, Irvine)35 (link). RNAi transgenic lines were obtained from the Vienna Drosophila RNAi Center (VDRC). For Glo1 knockdown, the 101560 line from the KK Library (phiC31-based transgenes at a single, defined site) was used. For Tpi knockdown, two lines were employed from the GD Library (P-element based, random insertion sites): 25643 and 25644. The UASeGFP and w; 3M; + (carrying an empty vector located in the KK site) lines were used in the experiment as titration controls.

Vicente Miranda H., Gomes M.A., Branco-Santos J., Breda C., Lázaro D.F., Lopes L.V., Herrera F., Giorgini F, & Outeiro T.F. (2016). Glycation potentiates neurodegeneration in models of Huntington’s disease. Scientific Reports, 6, 36798.

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Drosophila Genetics Toolbox Utilization

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The following stocks were obtained from Tsinghua RNAi Stock Center: UAS-babo-RNAi (THU5256), UAS- uba1-RNAi (THU2127).
The following stocks were obtained from Bloomington Stock Center (BSC): w1118, ok6-gal4, elav-gal4 (c155), PPK-Gal4, mCD8-GFP, UAS-EcR-RNAi (BSC9327), UAS-EcR-B1^DN (BSC9452), UAS-bsk-RNAi (BSC36643), UAS-mCherry-RNAi (BSC35785).
UAS-tubulin-GFP (Dr. Xing Liang, University of Tsinghua).

Xu W., Kong W., Gao Z., Huang E., Xie W., Wang S, & Rui M. (2023). Establishment of a novel axon pruning model of Drosophila motor neuron. Biology Open, 12(1), bio059535.

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Drosophila Stocks and Rearing Conditions

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w¹¹¹⁸, y'w’, OreR, ry⁵⁰⁶, elav::GeneSwitch, and elav::Gal4^c155 were obtained from the Bloomington Drosophila Stock Center. UAS::G6PD was generously provided by Dr. William C. Orr. Puc^E69 was a gift from Dr. Dirk Bohmann.
Female flies were used throughout. Flies were reared on yeast/molasses‐based food (agar [13.8 g], molasses [22 g], malt extract [75 g], dry yeast [Inactivated, 18 g], corn flour [80 g], soy flour [10 g], propionic acid [6.2 ml], methyl 4‐hydroxybenzoate [2 g], EtOH [7.2 ml], H₂O [1 L]) at 25°C, and 65% humidity on a 12‐hr light/dark cycle.

Wang L., Davis S.S., Borch Jensen M., Rodriguez‐Fernandez I.A., Apaydin C., Juhasz G., Gibson B.W., Schilling B., Ramanathan A., Ghaemmaghami S, & Jasper H. (2019). JNK modifies neuronal metabolism to promote proteostasis and longevity. Aging Cell, 18(3), e12849.

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Drosophila Neurodegeneration Transgenic Lines

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Flies were grown on standard cornmeal–agar–yeast-based medium at 25 °C. Transgenic fly lines bearing UAS-MJDtrQ27 (#8149), UAS-MJDtrQ78s (#8150), UAS-MJDtr-Q78w (#8141), UAS-GFP-IR (#9330), UAS-dRubicon (CG12772)-IR (#43276), UAS-GFP-mCherry-Atg8a (#37749), da-GAL4 (#55849) and elav-GAL4^c155 (#458) were obtained from the Bloomington Stock Center. Transgenic fly lines harbouring GMR-GAL4 were described previously^{51 (link)}.

Nakamura S., Oba M., Suzuki M., Takahashi A., Yamamuro T., Fujiwara M., Ikenaka K., Minami S., Tabata N., Yamamoto K., Kubo S., Tokumura A., Akamatsu K., Miyazaki Y., Kawabata T., Hamasaki M., Fukui K., Sango K., Watanabe Y., Takabatake Y., Kitajima T.S., Okada Y., Mochizuki H., Isaka Y., Antebi A, & Yoshimori T. (2019). Suppression of autophagic activity by Rubicon is a signature of aging. Nature Communications, 10, 847.

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