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Aspirin

Manufactured by Cayman Chemical
Sourced in United States

Aspirin is a crystalline compound that is commonly used as a starting material in various chemical reactions and analyses. It has the chemical formula C9H8O4 and is known for its ability to inhibit the production of certain prostaglandins, which are involved in the inflammatory response. Aspirin is widely used in research and development applications, but its specific use should be determined by the customer's intended application.

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11 protocols using aspirin

1

Antiplatelet and Anticoagulant Agents

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Prasugrel hydrochloride (prasugrel; CS-747S; molecular formula C20H20FNO3S·HCl) was obtained from Ube Industries, aspirin (molecular formula C9H8O4) was purchased from Cayman Chemical, and warfarin sodium (molecular formula C19H15NaO4) and Gum Arabic (vehicle) were purchased from Wako Pure Chemical Industries.
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2

Anti-VEGF and Aspirin Combo Therapy

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For long-term experiments, one day after ID8-VEGF tumor inoculation, mice were injected weekly with 40 μg anti-VEGF-A antibody (∼2 mg kg−1; clone G6-31; gift of Genentech), given daily gavage with 2 mg aspirin (∼100 mg kg−1; Cayman Chemical), or treated with both interventions. Control mice were given equal amounts of mouse IgG. For long-term experiments with anti-FasL antibody (clone MFL3), mice were given three 25 μg intratumoral injections a week.
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3

Blocking Innate Immune Receptors and Inflammatory Mediators

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Purified anti-human TLR2, TLR4 blocking antibodies and their matching isotype controls were obtained from BioLegend, Inc., USA. Bay 61–3606 and Phorbol 12-myristate 13-acetate (PMA), ticagrelor and Cell Detection ELISA PLUS kit were from Sigma-Aldrich, Australia. ML-171, aspirin, RGDS, cathepsin G inhibitor I, neutrophil elastase inhibitor (1-(3-methylbenzoyl)-1H-indazole-3-carbonitrile), myeloperoxidase inhibitor 1 (4-Aminobenzoic acid hydrazide), losartan were obtained from Cayman Chemical, USA. Abciximab (ReoPro) and low molecular weight heparin (Clexane enoxaparin sodium) were from Eli Lilly and Sanofi Aventis Australia Pty Ltd., respectively. DNAse I solution was purchased from STEMCELL Technologies Australia Pty Ltd. Collagen and thrombin were from Chrono-log Corporation, USA. Human PMN Elastase ELISA kit was obtained from Abcam Biotechnology, Cambridge, UK.
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4

Ticagrelor and Aspirin in MCAO Stroke Model

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Ticagrelor (LKT Laboratories, Inc., Saint Paul, Minnesota, USA) and aspirin (Cayman Chemical, Ann Arbor, Michigan, USA) were purchased. Mice were randomly divided into two ticagrelor treatment groups (10 mg/kg and 30 mg/kg), an aspirin treatment group (60 mg/kg), and a vehicle treatment group. Drugs were dissolved in 0.4 mL 1.0% carboxymethylcellulose and administrated by oral gavage at 3 and 24 hours before MCAO and 0 and 6 hours after reperfusion. Mice in vehicle treatment group were administered the same amount of carboxymethylcellulose at same time point.
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5

Anti-VEGF and Aspirin Combo Therapy

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For long-term experiments, one day after ID8-VEGF tumor inoculation, mice were injected weekly with 40 μg anti-VEGF-A antibody (∼2 mg kg−1; clone G6-31; gift of Genentech), given daily gavage with 2 mg aspirin (∼100 mg kg−1; Cayman Chemical), or treated with both interventions. Control mice were given equal amounts of mouse IgG. For long-term experiments with anti-FasL antibody (clone MFL3), mice were given three 25 μg intratumoral injections a week.
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6

Inhibiting Inflammatory Pathways in Foreskin HDFs

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NS-398 and aspirin were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Celecoxib was a generous gift from Dr. S.V. Yim (Kyung Hee University, Seoul, Korea). HDFs, isolated from foreskin [7 (link)], were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (Life Technologies, Carlsbad, CA, USA), penicillin (100 units/mL) and streptomycin (100 units/mL) in a 5% CO2 incubator [6 (link)].
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7

Caco-2 Cell Viability Assay for C. difficile

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Caco-2 cells were plated in black 96-well plates at 3 × 104 cells per well and incubated for 48 hours. After 48 hours, cell culture medium was refreshed, and cells were treated with indomethacin, ibuprofen (Cayman), naproxen (Cayman), aspirin, R2PPA, valeroyl silyciate (Cayman), celecoxib (Cayman), PGE2 (Cayman), or vehicle control [dimethyl sulfoxide (DMSO)] for 16 hours overnight. The next day, cells were intoxicated with filter-sterilized C. difficile VPI10463 cultures at a final dilution of 1:5 or 1:15 or mock-infected (BHI) and incubated for 8 hours. Cell viability was indicated by measuring ATP levels using the CellTiter Glo Reagent (Promega). Data were normalized to ATP levels of vehicle-treated and mock-infected cells.
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8

Purification and Characterization of Membrane Proteins

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Arachidonic acid (AA; 5Z, 8Z, 11Z, 14Z-eicosatetraenoic acid) and aspirin were purchased from Cayman Chemical Company (Ann Arbor, MI). Celebrex® was from a physician sample. [acetyl-14C] aspirin (55 mCi/mmol) was purchased from American Radiolabeled Chemicals (St. Louis, MO). Fe3+-protoporphyrin IX and Co3+-protoporphyrin IX were purchased from Frontier Scientific (Logan, UT). Decyl maltoside (C10M) was purchased from Anatrace (Maumee, OH), while n-octyl-β-D-glucopyranoside (βOG) was purchased from Inalco Pharmaceuticals (San Luis Obispo, CA). Anti-FLAG M2 affinity resin was purchased from Sigma-Aldrich (St. Louis, MO). FLAG peptide was purchased from GenScript (Piscataway, NJ). Polyacrylic acid (sodium salt) 5100 was purchased from Hampton Research. Restriction enzymes were purchased form New England Biolabs. The QuikChange™ II mutagenesis kit was purchased from Agilent Technologies (Santa Clara, CA). Oligos for site-directed mutagenesis, the Bac-to-Bac® Baculovirus expression kit and associated reagents, including fungizone and penicillin-streptomycin, were purchased from Invitrogen (Carlsbad, CA). SFM 921 serum free media, Spodoptera frugiperda 21 (Sf21) insect cells, and fetal bovine serum were purchased from Expression Systems (Davis, CA). HiTrap HP chelating and HiPrep Sephacryl 300-HR chromatography columns were purchased from GE Healthcare.
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9

Antibodies and Pharmacological Agents

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p53 (#2524), Bcl‐xL (#2762), and GAPDH (#2118) antibodies were purchased from Cell Signaling Technology. COX2 (#160106) antibody was purchased from Cayman Chemical. Doxorubicin was purchased from Sigma. Aspirin and celecoxib were purchased from Cayman Chemical.
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10

Antibiotic-Induced Clostridium difficile Infection

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All mouse procedures were approved by the Children’s Hospital of Philadelphia Institutional Animal Care and Use Committee. Mice were obtained from The Jackson Laboratory and were C57BL6/J males that were 4 weeks of age at arrival. Mice were acclimated for 7 days, and bedding was mixed upon arrival. After acclimation, mice were given cefoperazone at 0.5 g/liter in the drinking water for 5 days, with changes in antibiotic water every 2 days. After 5 days, water was changed to untreated water.
After the 5-day antibiotic treatment, mice were orally gavaged with indomethacin (Cayman) at 10 mg/kg, aspirin (Cayman) at 10 mg/kg, or R2PPA (Sigma-Aldrich) at 100 mg/kg of body weight or vehicle phosphate-buffered saline (PBS) for two consecutive days. Then, mice were infected with 105 spores of C. difficile ribotype Cd196. Mice were monitored for survival and were euthanized after reaching a terminal endpoint of appearing moribund or experiencing weight loss >20% from baseline. C. difficile colony-forming units were quantified daily from fecal samples. Samples were diluted and homogenized in PBS, serially diluted, and plated onto taurocholate cycloserine cefoxitin fructose agar. All animals were confirmed C. difficile culture negative upon arrival to the animal facility and on the day of infection.
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