Eclipse ti e inverted fluorescent microscope

Manufactured by Nikon

Sourced in Canada, Japan

The Eclipse Ti-E is an inverted fluorescent microscope designed for advanced live-cell imaging. It features a stable and precise optical system, providing high-resolution imaging capabilities. The microscope is equipped with a motorized stage and supports a range of fluorescent imaging techniques.

Automatically generated - may contain errors

Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (6 mentions) Lsm 780 confocal microscope, by Zeiss (3 mentions) Nucview 488 caspase 3 substrate, by Biotium (2 mentions) Calcein am, by Thermo Fisher Scientific (2 mentions) Collagenase 1, by Merck Group (2 mentions) Calcein am, by BMG Labtech (2 mentions) Calcein, by BMG Labtech (2 mentions) Hoechst33342, by BMG Labtech (2 mentions) Clariostar plate reader, by BMG Labtech (2 mentions) P creb, by Cell Signaling Technology (2 mentions) Brain derived neurotrophic factor (bdnf), by Novus Biologicals (2 mentions) Nis elements ar microscope imaging software, by National Instruments (1 mentions) Quanta 250 feg scanning electron microscope, by Thermo Fisher Scientific (1 mentions) Goat anti chicken igy alexa fluor 488 secondary antibody, by Thermo Fisher Scientific (1 mentions) Cryostat microtome cm 3050s, by Leica (1 mentions) Rorγt, by BD (1 mentions) Gata3, by Santa Cruz Biotechnology (1 mentions) Cm3050, by Leica (1 mentions) Cryostat microtome, by Leica (1 mentions) Bisbenzimide, by Merck Group (1 mentions)

Close spelling variants of this product

Eclipse Ti (2714 citations) Eclipse Ti microscope (1165 citations) Inverted microscope (910 citations) Eclipse Ti-E (736 citations) Eclipse Ti inverted microscope (513 citations) Ti-E microscope (435 citations) Ti-E inverted microscope (403 citations) Eclipse Ti-E microscope (321 citations) Eclipse Ti-E inverted microscope (293 citations) Eclipse microscope (191 citations)

11 protocols using eclipse ti e inverted fluorescent microscope

Evaluating Docetaxel Effects on Prostate Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

22Rv1 prostate cancer cells were seeded onto a collagen-fibronectin matrix in Stacks and treated with vehicle or docetaxel for 48 hours. Cells were then stained with Calcein AM (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA #C1430) and Hoechst33342 (Thermo Fisher Scientific, Waltham, MA, USA) for one hour followed by 1xPBS washes. Cells were extracted from each well by collagenase I (Sigma-Aldrich, USA) digestion and transferred into a 384-well low volume microplate. The fluorescence intensities of Calcein AM and Hoechst33342 staining were detected using a CLARIOstar Plate reader (BMG Labtech, Cary, NC, USA) at the Small Molecule Screening Facility, UW-Madison, and relative viability was established by comparing the Calcein signal in each condition to the control condition.
Caspase-3 activity was analyzed by fluorescence microscopy using NucView^® 488 Caspase-3 Substrate (Biotium, Fremont, CA, USA). Cells were imaged on a glass coverslip using a Nikon Ti-E Eclipse inverted fluorescent microscope. Quantification of caspase-3 activity were accomplished using NIS-Elements software. Spot detection function was used to identify individual cells and generate statistics on signal intensity values in each cell and for the sample as a whole. Reagents are listed in Supplemental Table I.

Sethakorn N., Heninger E., Breneman M.T., Recchia E., Ding A.B., Jarrard D.F., Hematti P., Beebe D.J, & Kosoff D. (2022). Integrated analysis of the tumor microenvironment using a reconfigurable microfluidic cell culture platform. The FASEB Journal, 36(10), e22540.

+ Open protocol

+ Expand

Enrichment and Characterization of Circulating Tumor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

CTCs enriched using the VERSA platform as described above were stained in the VERSA with Hoechst 33342 (Thermo Fisher, Cat# PI62249) and antibodies to HLA-A,B,C, EpCAM, CD27, CD45, CD34, and CD11b. Fluorophores, catalog numbers, and other antibody information is summarized in Supplementary Data S6. Patient characteristics for single-cell aspiration CTC samples are summarized in Supplementary Data S7. Cells were then further enriched using a single-cell aspiration platform, SASCA, previously developed by Tokar et al.^{39 (link)}. Briefly, cells were seeded into polydimethylsiloxane (PDMS) microwells mounted on a glass microscope slide. The microwell array was imaged on a Nikon Ti-E Eclipse inverted fluorescent microscope. CTCs were identified as EpCAM positive, exclusion (CD45/CD34/CD11b/CD27) negative cells, whereas WBCs were defined as EpCAM negative, exclusion positive cells. CTCs were further subdivided into groups based on HLA-I positivity compared to WBCs in the same sample. Target cells were aspirated from microwells and dispensed into a droplet of PBS in the EXTRACTMAN extraction plate (Gilson, Cat# 22100008) to proceed with DNA extraction. Microarray images were analyzed using NIS Elements AR Microscope Imaging Software (NIS-Elements, RRID:SCR_014329) to obtain HLA-I mean fluorescent intensity (MFI) values.

Rodems T.S., Heninger E., Stahlfeld C.N., Gilsdorf C.S., Carlson K.N., Kircher M.R., Singh A., Krueger T.E., Beebe D.J., Jarrard D.F., McNeel D.G., Haffner M.C, & Lang J.M. (2022). Reversible epigenetic alterations regulate class I HLA loss in prostate cancer. Communications Biology, 5, 897.

+ Open protocol

+ Expand

Prostate Cancer Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

22Rv1 prostate cancer cells were seeded onto a collagen‐fibronectin matrix in Stacks and treated with vehicle or docetaxel for 48 h. Cells were then stained with Calcein AM (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA #C1430) and Hoechst33342 (Thermo Fisher Scientific, Waltham, MA, USA) for 1 h followed by 1xPBS washes. Cells were extracted from each well by collagenase I (Sigma‐Aldrich, USA) digestion and transferred into a 384‐well low‐volume microplate. The fluorescence intensities of Calcein AM and Hoechst33342 staining were detected using a CLARIOstar Plate reader (BMG Labtech, Cary, NC, USA) at the Small Molecule Screening Facility, UW‐Madison, and relative viability was established by comparing the Calcein signal in each condition to the control condition.
Caspase‐3 activity was analyzed by fluorescence microscopy using NucView® 488 Caspase‐3 Substrate (Biotium, Fremont, CA, USA). Cells were imaged on a glass coverslip using a Nikon Ti‐E Eclipse inverted fluorescent microscope. Quantification of caspase‐3 activity was accomplished using NIS‐Elements software. Spot detection function was used to identify individual cells and generate statistics on signal intensity values in each cell and for the sample as a whole. Reagents are listed in Table S1.

+ Open protocol

+ Expand

Quantifying Protein Nanopatterns by Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

NOA63 lift-off stamps were imaged using Quanta 250 FEG scanning electron microscope (FEI, Japan) at 5 kV with a spot size of 3.5 using an ETD Detector to detect secondary electrons. Microand nanopatterns of fluorescently labeled protein were imaged on a Ti-E Eclipse inverted fluorescent microscope (Nikon, Japan) and an LSM 780 Confocal microscope (Zeiss, Japan). All images were captured with fixed exposure times within each experiment, which varied from 1 to 10 s for all the images shown in this work. Mean fluorescence intensity measurements were obtained by performing image analysis in ImageJ (NIH, USA). Images were processed post quantification to increase the contrast through linear modifications in ImageJ.

Sathish S., Ricoult S.G., Toda-Peters K, & Shen A.Q. (2017). Microcontact printing with aminosilanes: creating biomolecule micro- and nanoarrays for multiplexed microfluidic bioassays. The Analyst, 142(10).

+ Open protocol

+ Expand

Measuring Plaque Areas in CECs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plaque areas were measured in CECs exactly as previously described^{32 (link)} using anti-MDV chicken sera and goat anti-chicken IgY-Alexa Fluor 488 secondary antibody (Molecular Probes, Eugene, OR). Digital images of 36 individual plaques were collected using Nikon Eclipse-Ti-E inverted fluorescent microscope and plaque areas were measured using ImageJ³³ version 1.41o software. Whisker plots were generated using Microsoft Excel 365 and significant differences were determined using IBM SPSS Statistics Version 28 software Package (https://www.ibm.com/analytics/spss-statistics-software).

Akbar H., Fasick J.J., Ponnuraj N, & Jarosinski K.W. (2023). Purinergic signaling during Marek’s disease in chickens. Scientific Reports, 13, 2044.

+ Open protocol

+ Expand

Dual Immunofluorescence Staining of BDNF, p-CREB, and CREB

Check if the same lab product or an alternative is used in the 5 most similar protocols

The brain was frozen at –20°C, and sections were cut to a thickness of 20 μm using a Cryostat Microtome (CM 3050 S, Leica Microsystems, Wetzlar, Germany). We performed double immunofluorescence staining by incubating tissue sections with antibodies for BDNF (Novus Biologicals, Inc., Littleton, CO), p-CREB, and CREB (Cell Signaling Technology, Inc.) overnight at 4°C. Subsequently, FITC-conjugated secondary antibody was added for 2 h, and nuclear staining was performed using DAPI. Sections were observed using an Eclipse Ti-E inverted fluorescent microscope (Nikon Instruments Inc., Mississauga, Canada).

Park B.K., Kim Y.R., Kim Y.H., Yang C., Seo C.S., Jung I.C., Jang I.S., Kim S.H, & Lee M.Y. (2018). Antidepressant-Like Effects of Gyejibokryeong-hwan in a Mouse Model of Reserpine-Induced Depression. BioMed Research International, 2018, 5845491.

+ Open protocol

+ Expand

Immunohistochemical Staining of Lung Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemical staining was performed as previously described with minor modifications [64 (link)]. Briefly, the lung tissues were frozen at −20 °C, and the frozen sections were randomly chosen and sliced in 20 µm thicknesses using a cryostat microtome (CM 3050S, Leica Microsystems, Wetzlar, Germany). Lung tissue sections (20 µm) were fixed with 4% sucrose and 4% paraformaldehyde in PBS at 20–25 °C for 40 min, permeabilized with 0.5% Nonidet P-40 in PBS, and blocked with 2.5% horse serum and 2.5% bovine serum albumin for 16 h. Double immunofluorescence staining was performed by incubating lung tissue sections with antibodies against GATA-3 (Santa Cruz Biotechnology, CA, USA), RORγt (BD Biosciences) and Foxp3 (Abcam, Cambridge, U.K.) overnight at 4 °C. Subsequently, a fluorescein-conjugated secondary antibody was added and incubated for 2 h, and nuclear staining was performed using Hoechst stain. Sections were observed using an Eclipse Ti-E inverted fluorescent microscope (Nikon Instruments Inc., Mississauga, ON, Canada).

Kim S.H., Hong J.H., Yang W.K., Kim H.J., An H.J, & Lee Y.C. (2021). Cryptotympana pustulata Extract and Its Main Active Component, Oleic Acid, Inhibit Ovalbumin-Induced Allergic Airway Inflammation through Inhibition of Th2/GATA-3 and Interleukin-17/RORγt Signaling Pathways in Asthmatic Mice. Molecules, 26(7), 1854.

+ Open protocol

+ Expand

DAPI-Based Nuclear Morphology Analysis of HeLa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 4’,6-diamidino-2-phenylindole (DAPI) dye was used to evaluate the nuclear morphology of HeLa cells according to Morikawa and Yanagida (1981 (link)). The HeLa cells were exposed to EtOAc extract after growing to a density 4 × 10⁵ cells per well in a six-well plate for 24 h. The cells were fixed with 4% cold paraformaldehyde (PFA) in the dark for 15 to 20 min at room temperature and washed with PBS. After that, cells were stained with 4, 6-diamidino-2-phenylindole DAPI (10 µg/mL) and left for 30 min at room temperature. The nuclear morphology was acquired using a Nikon eclipse TiE inverted fluorescent microscope at a magnification of 100 X.

Verma J., Attri S., Arora S, & Manhas R.K. (2023). Antioxidant and chemoprotective potential of Streptomyces levis strain isolated from human gut. AMB Express, 13, 69.

+ Open protocol

+ Expand

Hippocampal BDNF and CREB Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

The hippocampus was frozen at –20°C and cut to a thickness of 20 μm using a Cryostat Microtome (CM 3050 S, Leica Microsystems, Wetzlar, Germany). For double immunofluorescence staining, the tissue sections were incubated with antibodies specific for BDNF (Novus Biologicals, Inc., Littleton, CO, USA), phosphorylated (p)-cAMP response element-binding protein (p-CREB), and CREB (Cell Signaling Technology, Inc., Danvers, MA, USA) overnight at 4°C. Subsequently, fluorescein isothiocyanate-conjugated secondary antibody was added for 2 h, and nuclear staining was performed with 4′,6-diamidino-2-phenylindole (DAPI). All tissue samples were observed with an Eclipse Ti-E inverted fluorescent microscope (Nikon, Tokyo, Japan).

Park B.K., Kim N.S., Kim Y.R., Yang C., Jung I.C., Jang I.S., Seo C.S., Choi J.J, & Lee M.Y. (2020). Antidepressant and Anti-Neuroinflammatory Effects of Bangpungtongsung-San. Frontiers in Pharmacology, 11, 958.

+ Open protocol

+ Expand

Immunofluorescence Staining of STAT3 in Lung Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

The lung tissues were frozen at −20 °C, and sections cut to a thickness of 20 µm using a Cryostat Microtome (CM 3050S, Leica Microsystems, Wetzlar, Germany). Lung sections (20 µm) were fixed with 4% paraformaldehyde and 4% sucrose in phosphate-buffered saline (PBS) at 20–25 °C for 40 min, permeabilized with 0.5% Nonidet P-40 in PBS, and blocked with 2.5% horse serum and 2.5% bovine serum albumin for 16 h at 20–25 °C. Double immunofluorescence staining was performed by incubating tissue sections with antibodies for STAT3 (Cell Signaling Technology, Inc. USA) overnight at 4 °C. Subsequently, fluorescein-conjugated secondary antibody was added for 2 h, and nuclear staining performed using Hoechst. Sections were observed using an Eclipse Ti-E inverted fluorescent microscope (Nikon Instruments Inc., Mississauga, Canada).

Kim S.H., Hong J.H., Yang W.K., Geum J.H., Kim H.R., Choi S.Y., Kang Y.M., An H.J, & Lee Y.C. (2020). Herbal Combinational Medication of Glycyrrhiza glabra, Agastache rugosa Containing Glycyrrhizic Acid, Tilianin Inhibits Neutrophilic Lung Inflammation by Affecting CXCL2, Interleukin-17/STAT3 Signal Pathways in a Murine Model of COPD. Nutrients, 12(4), 926.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!