Statistical evaluation was carried out using GraphPad Prism Software for Windows, version 8.0.0 (San Diego, CA, USA). Experiments were repeated two to three times in two to four technical replicates. In EC tissue gene expression was evaluated in 36 paired samples. Immunohistochemistry was performed on 56 tissue samples, where 20 were paired (pairs of EC and adjacent control tissue of the same patient). Statistical analysis was performed using the Kruskal–Wallis test with Dunn’s multiple comparison test correction (expression of ‘transporter genes’ in cell lines, siRNA silencing of SLCO1B3 and SLCO2B1), Wilcoxon matched pairs with Bonferroni’s multiple comparison test (expression of ‘transporter genes’ in paired EC tissue samples), one-way analysis of variance (ANOVA) with Bonferroni’s multiple comparison tTest (Western blotting), two-way ANOVA (analysis of association between transporters’ expression and histopathological and clinical data), Mann–Whitney U test (immunocytochemistry, [3H]E1-S transport and metabolism studies), paired t-test (immunohistochemistry). Differences of p < 0.05 were considered statistically significant. Unless stated otherwise, all data are shown as mean ± SD.
Prism software for windows
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GraphPad Prism is a software application for Windows that provides data analysis and graphing tools for scientific research. It allows users to analyze data, create publication-quality graphs, and perform statistical analyses. The software's core function is to assist researchers in visualizing and interpreting their data.
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Lab products found in correlation
20 protocols using prism software for windows
1
Transporter Gene Expression in Endothelial Cells
2
Statistical Analysis of Autoantibody Associations
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Association studies were carried out with contingency tables using the Chi2 test and Fisher’s exact test when Chi2 was not applicable.
The Baptista-Pike method was used to obtain the 95% confidence interval for Odds Ratio (OR).
Spearman’s rank correlation coefficient rho was used to measure the correlations between anti-CCP2 antibodies, AhFibA, and antibodies to the 5 peptides and tested against the null hypothesis of rho =0.
Statistical analysis was carried out using PRISM software for Windows (GraphPad Software, 169 San Diego, California, USA) and IBM®SPSS® Statistics version 20. The significance threshold was set at p < 0.05.
The Baptista-Pike method was used to obtain the 95% confidence interval for Odds Ratio (OR).
Spearman’s rank correlation coefficient rho was used to measure the correlations between anti-CCP2 antibodies, AhFibA, and antibodies to the 5 peptides and tested against the null hypothesis of rho =0.
Statistical analysis was carried out using PRISM software for Windows (GraphPad Software, 169 San Diego, California, USA) and IBM®SPSS® Statistics version 20. The significance threshold was set at p < 0.05.
3
Comparative Analysis of Neurological Outcomes
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All results are expressed as mean ± SEM. Two-way analysis of variance followed by Bonferroni’s post hoc test was used to compare the two groups. Student’s t-test was used whenever necessary. The level of significance was set at p < 0.05. All statistics and graphs were analyzed using Prism software for Windows, version 5.01 (GraphPad Software, San Diego, CA, USA).
4
Statistical Analysis of Experimental Data
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The statistical analysis was done using One-way or two-way ANOVA with bonferroni post test in GraphPad Prism Software for Windows, version 6.00, San Diego California USA (www.graphpad.com ). P<0.05 was the statistically significant cutoff P value.
5
Statistical Analysis of Experimental Data
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Results were obtained from at least three independent experiments. For statistical analysis, results are shown as mean±standard error of the mean. Using Prism software for Windows (GraphPad Software Inc., La Jolla, CA, USA), statistical significance of differences between groups was assessed by one-way analysis of variance (ANOVA) for factorial comparisons and by the Tukey's multiple comparison tests for multiple comparisons. Results of immunohistochemical staining were analyzed using the Mann–Whitney test. The differences in the positivity rates were evaluated with Fisher's exact test. Statistical analyses were carried out using the SPSS statistical software (ver. 12.0; SPSS Inc., Chicago, IL, USA).
6
Evaluating Shoulder Anatomical and Clinical Outcomes
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All statistical analyses were performed using GraphPad Prism Software for Windows (Version 8.0.1, San Diego, California, USA). Mean, median, range, standard deviation, and percentage were used as descriptive statistical methods to analyze the study data. The correlations between anatomical outcomes (preoperative, early postoperative, late postoperative CC distance, loosening percentage, and postoperative AC distance) and clinical outcomes (Constant Score [CS], ASES score, SSV score and pain score) were statistically evaluated using Pearson’s correlation test. Statistical significance level was set at P = .05 for all analyses.
7
Statistical Analysis of Experimental Data
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Statistical analyses were performed using the GraphPad Prism Software for Windows (version 8.0.1). Differences between groups were assessed using Student’s t-tests, and significant differences were marked in each figure as * < 0.05, ** < 0.01, and *** < 0.001.
8
Cardiac Ischemia-Reperfusion Injury Protocol
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Data were expressed as mean ± S.D. and processed by ANOVA and Bonferroni post-test for most of the comparison in cell cultures and animal studies. Repeated measures ANOVA II and Bonferroni post-test were used to analyze systolic blood pressure and the arrhythmia severity score during ischemia and reperfusion. The sample size required to detect a 50% reduction in reperfusion arrhythmias incidence is 10 animals per group. The incidence was compared using Fisher's exact test. The Prism software for Windows (version 9.2.0, GraphPad Software, San Diego, CA, USA) was used for all statistical analyses. A significance level of p < 0.05 was established.
9
Statistical Analysis of Gut Microbiome
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The results are expressed as the mean ± standard deviation. The normal distribution was performed using the Kolmogorov–Smirnov test. Two-tailed unpaired Student’s t-tests or Mann–Whitney tests were used when the data were normally or not normally distributed, respectively. Moreover, the Chi-squared test was used to compare the proportions of a sufficiently large number of species (˃20 in each contingency table cell). In contrast, Fisher’s exact and bilateral Barnard’s exact tests were used if the number of species was small (typically < 20 in one or more cells of the table). For gut microbiota analysis, differences in the relative abundances of OTUs of dominant bacteria were analyzed using Mann–Whitney U-tests. Statistical significance was accepted at p < 0.05. All analyses were performed with GraphPad Prism Software for Windows (GraphPad Software, San Diego, CA, USA) (version 9.0).
10
Statistical Analyses of Experimental Data
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All statistical analyses were conducted using a two-way analysis of variance with Dunnett’s comparison tests or unpaired t-tests. These calculations were carried out using GraphPad Prism Software for Windows (GraphPad Software, San Diego, CA, USA). Significance was observed at p < 0.05.
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