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Hpa003259

Manufactured by Merck Group
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HPA003259 is a laboratory equipment product manufactured by Merck Group. It is a versatile device used for various scientific applications. The core function of this product is to perform specific tasks required in a laboratory setting. However, a detailed description of its intended use cannot be provided in an unbiased and factual manner without extrapolation.

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Lab products found in correlation

Anti h3k27ac, by Diagenode (2 mentions) C15410196, by Diagenode (2 mentions) Ab12039, by Abcam (1 mentions)

4 protocols using hpa003259

1

Protein-Protein Interactions in Melanogenesis

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Cells were grown on glass coverslips, fixed and permeabilized. PLA (Duolink) was performed according to the manufacturer’s instructions (Sigma) using antibodies against ARAF (cs4432, Cell Signaling) and MITF (ab12039, abcam), BRAF (F7, Santa Cruz) and MITF (HPA003259, Sigma), or CRAF (610151, BD Biosciences) and MITF (HPA003259, Sigma). Images were captured using a 3D Leica fluorescence microscope. The average number of dots per cell (identified by its nucleus stained with DAPI) was determined by analysing at least 148 different cells with Image J software.
Estrada C., Mirabal-Ortega L., Méry L., Dingli F., Besse L., Messaoudi C., Loew D., Pouponnot C., Bertolotto C., Eychène A, & Druillennec S. (2022). MITF activity is regulated by a direct interaction with RAF proteins in melanoma cells. Communications Biology, 5, 101.
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2

Transcriptional and Protein Profiling of the Developing Eye

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RNA in situ hybridisation (ISH) of cryostat sections was performed as previously described (Reissmann et al., 1996 (link)). Antisense RNA probes specific for chicken Pax6, Mitf, MMP115, Vsx2, Wnt2b (Müller et al., 2007 (link)) were used. For immunohistochemical studies, antibodies recognizing MITF (HPA003259, Sigma-Aldrich), pSMAD1/5/8 (9516, 9511S, Cell Signaling Technologies), PY489-β-Catenin (DSHB, Iowa, USA), MMP115 (Makato Mochii, Hyogo, Japan), Retinal pigment epithelial-specific protein 65 kDa (4018BD113D9, Novus Biologicals, Littleton, USA), VSX2 (Thermo Fisher Scientific), BRN3α (Eric Turner, University of California, San Diego, USA), Visinin, TUJ1, Collagen IX and G3G4 anti-BrdU (DSHB) were used on cryostat sections (Steinfeld et al., 2013 (link)). AChE activity was visualized according to the methods described by Karnovsky and Roots (1964) (link). Images were taken with a Stereomicroscope Nikon H550L, Axiovert S1002 and Axiophotx10 Observer D1 (Carl Zeiss) and processed with Adobe Photoshop CS5 (Adobe Systems).
Steinfeld J., Steinfeld I., Bausch A., Coronato N., Hampel M.L., Depner H., Layer P.G, & Vogel-Höpker A. (2017). BMP-induced reprogramming of the neural retina into retinal pigment epithelium requires Wnt signalling. Biology Open, 6(7), 979-992.
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3

Chromatin Immunoprecipitation Sequencing

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ChIP-Seq was performed as previously described [26 (link)], using anti-H3K27ac (C15410196, Diagenode) or anti-MITF (Sigma, HPA003259). Details of ChIP-seq and data analysis are described in the supplemental information. The human tissue samples used in this study were collected on protocol 2007P000699 “Culture of LAM, AMLs, and other TSC Lesions” approved by the Partners Human Research Committee and informed consent was obtained from all subjects.
Additional methodologic details for metabolomic profiling, whole transcriptome RNA sequencing and immunohistochemistry are provided in supplemental information.
Zarei M., Giannikou K., Du H., Liu H.J., Duarte M., Johnson S., Nassar A.H., Widlund H.R., Henske E.P., Long H.W, & Kwiatkowski D.J. (2020). MITF is a driver oncogene and potential therapeutic target in kidney angiomyolipoma tumors through transcriptional regulation of CYR61. Oncogene, 40(1), 112-126.
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4

Chromatin Immunoprecipitation Sequencing

Cited 1 time
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ChIP-Seq was performed as previously described [26 (link)], using anti-H3K27ac (C15410196, Diagenode) or anti-MITF (Sigma, HPA003259). Details of ChIP-seq and data analysis are described in the supplemental information. The human tissue samples used in this study were collected on protocol 2007P000699 “Culture of LAM, AMLs, and other TSC Lesions” approved by the Partners Human Research Committee and informed consent was obtained from all subjects.
Additional methodologic details for metabolomic profiling, whole transcriptome RNA sequencing and immunohistochemistry are provided in supplemental information.
Zarei M., Giannikou K., Du H., Liu H.J., Duarte M., Johnson S., Nassar A.H., Widlund H.R., Henske E.P., Long H.W, & Kwiatkowski D.J. (2020). MITF is a driver oncogene and potential therapeutic target in kidney angiomyolipoma tumors through transcriptional regulation of CYR61. Oncogene, 40(1), 112-126.
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