Hepatocellular carcinoma cell line Hep3B (CL‐0102) and human hepatic stellate cell line LX‐2 (CL‐0560) were purchased from Procell. Huh7 cells were purchased from the Chinese Academy of Sciences Shanghai Cell Bank. SNU‐449 cells were purchased from the ATCC. LX‐2, SNU‐449, and Huh7 cells were cultured in DMEM medium (Gibco) supplemented with 10% FBS (Invitrogen). Hep3B cells were cultured in MEM (Gibco) containing 10% FBS (Invitrogen). All cells were cultured at 37 °C in 5% CO2.
Hep3b
Manufactured by Procell
Sourced in China
The Hep3B is a cell line derived from human hepatocellular carcinoma. It is commonly used in biomedical research as an in vitro model for the study of liver-related diseases and processes.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by Procell (12 mentions)
Sk hep 1, by Procell (8 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (7 mentions)
Hep3b, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Procell (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Thle 2, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Procell (2 mentions)
Bronchial epithelial growth medium (begm), by Lonza (2 mentions)
Rpmi 1640 medium, by Procell (2 mentions)
Thle 2, by Lonza (2 mentions)
Plcprf5, by Procell (2 mentions)
Mhcc97 h, by Procell (2 mentions)
Sk hep1, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Sartorius (2 mentions)
Smmc 7721, by Procell (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Hcclm3, by Procell (2 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
29 protocols using hep3b
1
Hepatocellular Carcinoma Cell Lines
2
Culture of Liver Cell Lines
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The HCC cell lines (Huh7 and Hep3B) were bought from Procell (Wuhan, China) and the human normal liver cell line (THLE-2) was bought from the American Type Culture Collection (ATCC, Manassas, VA, USA). All cells were kept in Dulbecco's modified Eagle's medium (DMEM; Procell) added with 10% fetal bovine serum (FBS; Procell) and 1% penicillin-streptomycin (Procell) at 37°C in a humid incubator consisting of 5% CO 2 .
3
Assessing KRT15 Expression in Liver Cell Lines
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The normal liver cell line THLE-2 (Cat. No. iCell-h38) cell lines were provided by the iCell Bioscience Inc. and liver cancer cell lines including Huh7 (Cat. No. CL-0120), PLC (Cat. No. CL-0415), Hep3B (Cat. No. CL-0102), HepG2 (Cat. No. CL-0103), and Li-7 (Cat. No. CL-0139) were obtained from Procell Life Science & Technology Co., Ltd. Cells of THLE-2 were maintained in BEGM (Lonza Group, Ltd.), cells including PLC, Hep3B, HepG2 and Li-7 were maintained in RPMI-1640 medium (Procell Life Science & Technology Co., Ltd.) and Huh7 cells were maintained in DMEM (Procell Life Science & Technology Co., Ltd. The medium was supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich; MilliporeSigma) and 1% penicillin/streptomycin (Procell Life Science & Technology Co., Ltd.). All cells were cultured in a humidity incubator with 5% CO2 at 37°C. The expression level of KRT15 in these cells was assessed using reverse transcription-quantitative PCR (RT-qPCR) and western blot analyses.
4
Establishing Stable Gene Modulation in HCC Cells
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The HCC cells including PLC/PRF/5, Huh7, HepG2, Hep3B, and MHCC-97L cells, as well as immortalized human hepatocytes (MIHA cells), were obtained from the Procell Life Science and Technology Company in China. Cells were seeded in culture plates with the RPMI 1640 medium (HyClone, USA) with 10% (v/v) fetal bovine serum (FBS) (Gibco, USA) at a humid incubator (37° C and 5% CO2). The cells were trypsinized by using 0.25% trypsin (Gibco, USA) and sub-cultured when cells reached 70-80% confluence. For the establishment of HCC cell lines with the stable knockdown/overexpression of corresponding genes, indicated constructs were induced into HCC cells through lentiviral infection. After that, cells were screened by adding 3 μg/ml puromycin or 5 μg/ml blasticidin.
5
Subcutaneous Xenograft Model in Nude Mice
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6 week-old male BALB/c nude mice were purchased from the SLAC Jingda Laboratory Animal Co.Ltd (Hunan, China). All animal experiments were performed under specific sterile barrier conditions. All protocols were approved by the Animal Ethics Committee (No. 2021049). A total of 5×106 cells resuspended in 100 μL of Matrigel were subcutaneously transplanted into nude mice. Lenvatinib (10 mg/kg) was administered orally 5 times a week when the tumors reached approximately 100 mm3. Tumors were measured every 3 days, and tumor volume was determined using the formula length∗widthˆ2/2. 293T cell line was obtained from the Chinese Academy of Sciences (Shanghai, China). Huh-7 and Hep3B were purchased from Procell (Wuhan, China). HCCLM3 was purchased from the Zhong Qiao Xin Zhou Biotechnology (Shanghai, China).The companies have authenticated these cell lines and test these cell line for mycoplasma prior to sale.
6
Culturing Human Liver Cancer Cell Lines
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Cell lines human liver cancer Hep3B and MHCC97H cells were purchased from Procell Life Science & Technology Co. Ltd. (Wuhan, China) and the Cell Bank of Chinese Academy of Sciences (Shanghai, China), respectively. Human embryonic kidney 293T (HEK293T) cells were gifted by Li Zhong (Chongqing University). HEK293T, Hep3B and MHCC97H cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing L-glutamine supplemented with 10% fetal bovine serum (FBS), and penicillin (100 U/ml)/streptomycin (100 μg/ml). Cells were grown in a humidified incubator at 37 °C in the presence of 5% CO2. Transfections were performed using Lipofectamine 2000 (Invitrogen; 11668-019) according to the manufacturer's instructions.
7
Lentiviral Delivery of SGOL2 and MAD2 in HCC Cells
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The HCC cell lines SK-HEP-1 and HEP3B were purchased from Procell (Wuhan, China). All cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco), 1% streptomycin, and penicillin. Cells were transfected with lentivirus or plasmid purchased from Shanghai Genomeditech (Shanghai, China) and verified by DNA sequencing. Lentiviruses containing shNC (negative control, NC) and shSGOL2 were constructed using the vector pGMLV-SC5. The shRNA sequence used to target SGOL2 was as follows: 5′-GGTCAGAATTCCCTAACTTGT-3′. The pGMLV-SGOL2 plasmid contained the SGOL2 coding sequence, and the pGMLV-MAD2 plasmid contained the MAD2 coding sequence. For plasmid transfection, SK-HEP-1 and HEP3B cells were seeded in 12-well plates and then transfected with plasmids (4 mg per well) using Lipofectamine 2000 Reagent (Invitrogen) according to the protocols. Cells were harvested for analysis after 48 h.
8
Culturing Hepatic Cell Lines and Modulating CFHR3 Expression
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All HCC cell lines (Hep3B, Huh-7, JHH-7, Li-7, QGY-7701, SK-Hep-1, SNU-398, SNU-423, HepG2 and SMMC-7721) and normal hepatic epithelial cells (LO2) were obtained from Procell (Wuhan, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Biological Industries, Israel) containing 10% fetal bovine serum (FBS; Biological Industries, Israel) at 37°C with 5% CO2. CFHR3 plasmids and their corresponding vectors were obtained from iGene Biotechnology Co. Ltd. (Beijing, China). The normoxic environment was set as 21% O2, 5% CO2 and 74 N2 in a three-gas incubator, while the hypoxic environment was set as 1% O2, 5% CO2 and 94 N2. Transfection of plasmids and vectors was performed using Lipofectamine 2000 (Thermo Scientific, USA) for 6 h, according to the manufacturer’s protocol. To obtain stable CFHR3-overexpressing cells, they were cultured with 0.5 μg/mL puromycin for 10 d.
9
Cell Culture Conditions for Liver Cancer Lines
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Hep3B, Huh7, PLCPRF5, Li7, and THLE2 cells were purchased from ProCell (Wuhan, China). The THLE2, PLCPRF5 and Hep3B were cultured with Minimum Essential Medium. Huh7 was cultured with Dulbecco’s Modified Eagle Medium. Li7 was cultured with Roswell Park Memorial Institute-1640. These medium was added with 10% FBS and 1% penicillin–streptomycin. Incubation was carried out in an environment with 5% CO2 at a temperature of 37 °C.
10
Culturing Human Liver Cell Lines
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Human HCC cell lines, HepG2, Hep3B and Huh7 were obtained from Procell Life Science and Technology Co., Ltd. (Wuhan, China), and L02 cells were purchased from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). Cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; M&C Gene Technology Co., Ltd., Beijing, China) and supplemented with 10% (v/v) fetal bovine serum (Gibco Life Technologies, Waltham, MA, USA), 100 U/mL of penicillin, and 100 U/mL of streptomycin. Both cells were incubated in a humidified 5% (v/v) CO2 at 37°C incubator.
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