Xevo tq s mass detector

A Waters ACQUITY UPLC and Xevo TQ-S Mass Detector (Waters company, Milford, MA, USA) was used as UPLC-MS/MS system. Chromatographic separation was achieved on a C18 reverse-phase column (ACQUITY UPLC^® BEH Phenyl column 1.7 μm, 50 mm × 2.1 mm). The column temperature was set at 40 °C. Gradient elution was established with a mobile phase consisting of methanol (eluent A) and 0.1% (v/v) formic acid in water (eluent B). The injection volume was 2 μL, and the flow rate was 0.35 mL/min. At the start of the run, A ran at 15% for 1.5 min, increasing linearly from 15 to 90% for 1.5 to 3 min and then reduced linearly from 90 to 15% for 3 to 5 min. A ran at 15% for one minute, a total run time of 6 min. MS analyses were carried out using multiple reaction monitoring (MRM) with an electrospray ionization (ESI) source in the positive mode. The MS parameters are provided in Table 1, and the MRM parameters are provided in Table 2. MassLynx version 4.0 software running in Microsoft Windows 7 Professional was used to operate the instrument for data acquisition and data processing for automated quantification.

A Waters ACQUITY high-performance LC system equipped with a Waters Xevo TQS mass detector was employed for anthocyanins analysis [44 (link)]. The separation chromatographic column was a reversed-phase Hypersil GOLD C18 (100 mm × 2.1 mm, 1.9 μm; Thermo Scientific, Waltham, MA, USA). The mobile phase consisted of two solvents: formic acid (0.5%, v/v) in water (A) and methanol (B) with gradient elution at a flow rate of 0.3 mL/min. The gradient program of the mobile phase was as follows: 0–2 min, 5% B; 2–5 min, 5–10% B; 5–10 min, 10–16% B; 10–12 min, 16–95% B; 12–15 min, 95% B; 15–16 min, 95–5% B; 16–20 min, 5% B. The injection volume was 3 μL, and the column temperature was maintained at 40 °C.
The mass spectrometer was operated in a positive-ion mode by scanning ions between m/z 100 and 1000. The ESI inlet conditions were as follows: capillary voltage, 0.6 kV; cone voltage, 30 V; extractor voltage, 2 V; source temperature, 120 °C; and desolvation temperature, 350 °C. Data acquisition of anthocyanins and further determinations were done by the OS 1.8 software (Waters).