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Anti rad50

Manufactured by Cell Signaling Technology
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Anti-Rad50 is a laboratory research reagent produced by Cell Signaling Technology. It is a polyclonal antibody that recognizes the Rad50 protein, which is a key component of the MRE11-RAD50-NBS1 (MRN) complex involved in DNA double-strand break repair and telomere maintenance.

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Lab products found in correlation

Anti mre11, by Cell Signaling Technology (2 mentions) Anti cip2a, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Anti lamin a c, by Santa Cruz Biotechnology (1 mentions) Anti brca1, by Santa Cruz Biotechnology (1 mentions) Anti topbp1, by Fortis Life Sciences (1 mentions) Anti mdc1, by Abcam (1 mentions) Anti ha tag, by Novus Biologicals (1 mentions) Anti 53bp1, by Thermo Fisher Scientific (1 mentions) Anti lamin b1, by Santa Cruz Biotechnology (1 mentions) Anti mre11, by GeneTex (1 mentions) Anti lig4, by GeneTex (1 mentions) Mouse anti phospho histone h2a x, by Merck Group (1 mentions) Goat anti pml n 19, by Santa Cruz Biotechnology (1 mentions) Rabbit anti mad1, by GeneTex (1 mentions) Rabbit anti nbs1, by Novus Biologicals (1 mentions) Anti beta actin ac 15, by Abcam (1 mentions) Anti phospho akt, by Cell Signaling Technology (1 mentions) Anti cleaved parp, by Cell Signaling Technology (1 mentions) Anti γh2ax, by Merck Group (1 mentions)

Close spelling variants of this product

Rad50 (23 citations) Anti-phospho-Rad50 (Ser635) (2 citations) Rabbit anti-RAD50 (2 citations)

5 protocols using anti rad50

1

Antibody Panel for DNA Damage Response

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Following antibodies were used in this study at the indicated concentration. Anti-phospho histone H2AX (Ser139) (γH2AX) (clone JBW301, EMD Millipore, 05–636, 1:1500 IF) (Cell Signaling Technologies, 2577, 1:1000 IF), anti-TOPBP1 (Bethyl Laboratories, A300–111A, 1:2000 IF, 1:2000 IB) (Abcam, ab2402, 1:1000 IF), anti-MDC1 (Abcam ab11171, 1:1000 IF, 1:1000 IB), anti-CIP2A (clone 2G10–3B5; Santa Cruz sc80659, 1:500 IF, 1:1000 IB), anti-GAPDH (clone 14C10, Cell Signaling Technologies, 1:5000 IB), anti-PolD3 (clone 3E2, Abnova, H00010714-M01, 1:1000 IB), anti-Lig4 (clone N2C2, GeneTex, GTX100100, 1:1000 IB), anti-Mre11 (clone 12D7, GeneTex, GTX70212, 1:1000), anti-Rad50 (Cell Signaling Technologies, 3427, 1:1000 IB), anti-HA-tag (Novus biologicals, NB600–363, 1:2000 IB), anti-Lamin A/C (clone E-1, Santa Cruz, sc-376248, 1:200 IF), anti-Lamin B1 (clone C-5, Santa Cruz, sc-365962, 1:200 IF), anti-53BP1 (Thermo Fisher Scientific, PA1–16565, 1:1000 IF), anti-BRCA1 (clone D-9, Santa Cruz, sc-6954, 1:1000 IF).
Broberger C., Johansen J., Johansson C., Schalling M, & Hökfelt T. (1998). The neuropeptide Y/agouti gene-related protein (AGRP) brain circuitry in normal, anorectic, and monosodium glutamate-treated mice. Proceedings of the National Academy of Sciences of the United States of America, 95(25), 15043-15048.
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2

Comprehensive Antibody Panel for DNA Damage

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Rabbit anti-PARP-1 (H-250), anti-caspase-3 (H-277) and anti-POT1 (H-200) (Santa Cruz, CA, USA); goat anti-PML (N-19) (Santa Cruz); mouse anti-p53 (DO-1), anti-Cyclin B1 (GNS1), anti-N-myc (B8.4.B) and anti-TRF2 (4A794) (Santa Cruz); rabbit anti-PARP-9, anti-PML, anti-c-Myc (Y69); mouse anti-Rad50 (13B3/2C6), anti-Mre11 (12D7) and anti-NBS1 (NBS1-501) (Abcam, Cambridge, MA, USA); rabbit anti-phospho-histone H2A.X (20E3), anti-p21 (12D1) and anti-Rad50 (Cell Signaling, MA, USA); mouse anti-TRF2 (Cell Signaling); mouse anti-phospho-histone H2A.X (Millipore); rabbit anti-MAD1 (Gene Tex, CA, USA), rabbit anti-NBS1(Novus, Cambridge, UK) and anti-beta actin (AC-15) (Abcam) antibodies were used.
Yu Y., Katiyar S.P., Sundar D., Kaul Z., Miyako E., Zhang Z., Kaul S.C., Reddel R.R, & Wadhwa R. (2017). Withaferin-A kills cancer cells with and without telomerase: chemical, computational and experimental evidences. Cell Death & Disease, 8(4), e2755-.
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3

Molecular Targets in Cancer Research

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Anti-NF-κB, anti-Bcl-2, anti-cyclin A, anti-cyclin B, anti-cyclin E, anti-phospho-Raf-1, anti-Akt, anti-Snail, and anti-β-actin were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-cleaved PARP, caspase-3, anti-ATM, anti-ATR, anti-Rad50, anti-p95/NBS1, anti-Rad52, anti-MRE11, anti-Ku70, anti-Ku80, anti-DNA-PKcs, anti-ERCC1, anti-Rad51, anti-Ras, anti-MEK1/2, anti-phospho-MEK1/2, anti-Erk1/2, anti-phospho-Erk1/2, anti-phospho-Akt, anti-vimentin, anti-E-cadherin, and anti-MMP9 were purchased from Cell Signaling Technology (Danvers, MA, USA), and anti-γ-H2AX was obtained from Millipore (Billerica, MA, USA). ZOL was purchased from Sigma-Aldrich (St. Louis, MO, USA). For in vitro experiments, ZOL was dissolved in PBS to make a 2 mmol/L stock solution and stored at −20°C.
Kim E.H., Kim M.S., Lee K.H., Koh J.S., Jung W.G, & Kong C.B. (2016). Zoledronic acid is an effective radiosensitizer in the treatment of osteosarcoma. Oncotarget, 7(43), 70869-70880.
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4

Antibody Characterization and Validation

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Commercial antibodies used were as follows: anti-FLAG (Sigma), anti-CDYL (Sigma, HPA035578; for Western blotting), anti-CDYL (Abcam, ab5188), anti-BrdU (Abcam, ab6326), anti-RPA1 (ABclonal, A0990), anti-RAD51 (ABclonal, A6268), anti-WRN (ABclonal, A6855), anti-DNA2L (Abcam, ab2179), anti-BLM (Abcam, ab96488), anti-MRE11 (Cell Signaling Technology, 4847), anti-NBS1 (Cell Signaling Technology, 14956), anti-RAD50 (Cell Signaling Technology, 3427), anti-Myc, anti-hemagglutinin (HA), anti–β-actin (MBL), anti-PanKac (PTM, 101), anti-PanKcr (PTM, 502; for Western blotting), and anti-PanKcr–conjugated agarose beads (PTM, 503; for immunoprecipitation). A CDYL antibody that we generated previously (peptide antigen: KQKESTLTRTNRTSPNN; B&M) was used for immunoprecipitation. The polyclonal antibodies against RPA1 K88cr, RPA1 K379cr, and RPA1 K595cr were generated by immunizing rabbits with two synthetic crotonyl peptides corresponding to residues surrounding K88, K379, and K595 of RPA1, respectively. HU, CPT, and VP16 were from Sigma. Streptavidin agarose resins were from Thermo Fisher Scientific. Protein A/G and Sepharose CL-4B beads were from GE Healthcare Biosciences, and protease inhibitor mixture cocktail was from Roche Applied Science.
Yu H., Bu C., Liu Y., Gong T., Liu X., Liu S., Peng X., Zhang W., Peng Y., Yang J., He L., Zhang Y., Yi X., Yang X., Sun L., Shang Y., Cheng Z, & Liang J. (2020). Global crotonylome reveals CDYL-regulated RPA1 crotonylation in homologous recombination–mediated DNA repair. Science Advances, 6(11), eaay4697.
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5

DNA Damage Response Protein Analysis

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Anti-BRCA1 (D-9), anti-CtIP (T-16), anti-Cdk1/2 (AN21.2), anti-Chk1 (G-4), anti-histone H1 (N-16), anti-Mre11 (C-16), anti-Rad51 (H-92), and anti-HSC70 (K-19) antibodies were purchased from Santa Cruz Biotechnology. Anti-phospho-Chk1 (Ser-345), anti-phospho-CDK1/2 (Tyr-15), anti-Nbs1, anti-Rad50, and anti-γ-H2AX-Alexa Fluor 488 antibodies were from Cell Signaling. Anti-phospho-RPA32 (S4/S8) and anti-RPA32 antibodies were from Bethyl Laboratories (Montgomery, TX). Anti-phospho-histone H1 was from EMD Biosciences. Anti-RAP80 was from Novus Biochemicals (Littleton, CO).
Lin Z.P., Ratner E.S., Whicker M.E., Lee Y, & Sartorelli A.C. (2014). Triapine Disrupts CtIP-mediated Homologous Recombination Repair and Sensitizes Ovarian Cancer Cells to PARP and Topoisomerase Inhibitors. Molecular cancer research : MCR, 12(3), 381-393.
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