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L glutamic acid

Sourced in China

L-Glutamic acid is a biochemical compound used as a laboratory reagent. It is an amino acid that plays a key role in various metabolic processes. The product provides a source of this important biomolecule for research and analytical applications.

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4 protocols using l glutamic acid

1

Amino Acid and Compound Acquisition Protocol

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L-Alanine (CAS No. 56-41-7), L-Arginine (CAS No. 74-79-3), L-Asparagine (CAS No. 70-47-3), L-Aspartic acid (CAS No. 56-84-8), L-Cysteine (CAS No. 52-90-4), L-Glutamic acid (CAS No. 56-86-0), L-Glutamine (CAS No. 56-85-9), L-Histidine (CAS No. 71-00-1), L-Isoleucine (CAS No. 73-32-5), L-Leucine (CAS No. 61-90-5), L-Lysine (CAS No. 56-87-1), L-Methionine (CAS No. 63-68-3), L-Phenylalanine (CAS No. 63-91-2), L-Proline (CAS No. 147-87-3), L-Serine (CAS No. 56-45-1), L-Threonine (CAS No. 72-19-5), L-Tryptophan (CAS No. 73-22-3), L-Tyrosine (CAS No. 60-18-4), L-Valine (CAS No. 72-18-4), and Glycine (CAS No. 56-40-6) were purchased from MACKLIN. MHY1485 (CAS No. 326914-06-1) and Rapamycin (CAS No. 53123-88-9) were purchased from APExBIO.
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2

Dialysis Characterization of Biomolecules

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GelNB-loaded molecular weight 3500 Da dialysis bags with deionized water were sealed by clips and immersed in 500 mL of d-Glucose (Meilunbio, China), l-arginine (Sangon Biotech, China), l-glutamic acid (Macklin, China), l-tryptophan (Macklin), glycine (Macklin), l-glutamine (Macklin), l-cysteine (Macklin), or Ringer's solution, respectively. As a control, deionized water was placed in a separate clean dialysis bag. The liquid in the dialysis bags was collected in a vacuum tube at regular intervals as a dialysis test sample. The amino acid concentrations were determined using a total amino acid assay kit (Nanjing Jiancheng Bio-engineering Institute, China). The glucose concentration was assessed using a glucose colorimetric assay kit (Elabscience, China). The ion concentrations were measured using an automatic biochemical analyzer (Beckman Coulter AU5800, USA).
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3

Enzymatic Lactate Sensing Platform

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Lactate (90%), urea (99%), uric acid (99%), bovine serum albumin (BSA), and L-glutamic acid were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). Tris(hydroxymethyl)aminomethane (Tris), N-acetyl-L-cysteine, and L-cysteine were purchased from Sigma-Aldrich. BaCl2·2H2O, KCl, Sinopharm Chemical Reagent Sodium offered sodium borohydride (NaBH4) and isopropano. Shanghai Chemical Reagents Company (Shanghai, China) supplied CdCl2·2.5H2O, Na2TeO3, and CaCl2·2H2O. Monodisperse fluorescent microspheres (3 μm) were obtained from Aladdin Industrial Inc. (Shanghai, China). Tianjin Heowns OPDE Technologies, LLC (Tianjin, China) supplied lactate oxidase (90 units/mg). Sodium alginate was purchased from Alfa Aesar. Zhongnan Hospital of Wuhan University offered human serum samples. All the experimental solutions were prepared with ultrapure water, which was obtained from a Millipore water purification system.
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4

Chiral Compound Synthesis and Characterization

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All reagents and solvents were purchased from commercial sources and used as received without further purification. Melamine (C3H6N6, 99%), potassium ferricyanide (K3[Fe(CN)6], AR), (1R)-(−)-10-CSA (99%), (1S)-(−)-10-CSA (99%), L-phenylalanine (99%), D-phenylalanine (98%), L(+)-glutamic acid (99%), D-glutamic acid (98%), L(−)-carvone (99%), S-(+)-carvone (97%), +CAM, 99% and TAD, 98% were purchased from Shanghai Macklin Biochemical Co. Ltd. (China). (+)-limonene (>95%), (−)-limonene (>95%) were purchased from Tokyo Chemical Industry (TCI) Co. Ltd (Japan). NDA, 98% was purchased from ARK Pharm, Inc., USA. Ethanol (AR, ≥99.7%), concentrated sulfuric acid (AR, 95–98%), sodium chloride (NaCl, AR) and magnesium chloride hexahydrate (MgCl2·6H2O, AR) were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). Sucrose (AR), Rhodamine B (RhB, AR), methyl orange (MO, 96%), alizarin yellow R (AYR, AR), L-tryptophan (99%) and D-tryptophan (98%) were purchased from Aladdin Industrial Corporation (China). Fe(phen)3Cl2 was synthesized according to previous report 28 (link).
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