PATU8988 and PANC‐1 cells were harvested in ice‐cold PBS and lysed in radioimmunoprecipitation assay (PIRA) buffer supplemented with protease inhibitors. Protein concentration was determined using the BCA protein assay kit (Rockford, IL, USA) and equal amounts of proteins were analyzed using SDS–PAGE (10% acrylamide gel). The antibodies used for Western blot were a GAPDH rabbit mAb antibody (Santa Cruz Biotechnology San Francisco, CA, USA) and PDCD4 and PTEN rabbit mAb antibodies (Cell Signal Technology, Danvers, MA).
Gapdh rabbit mab antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
GAPDH rabbit mAb antibody is a monoclonal antibody that recognizes the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a key enzyme involved in the glycolytic pathway and is commonly used as a loading control or reference protein in various biochemical and cell biology applications.
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Lab products found in correlation
2 protocols using gapdh rabbit mab antibody
1
Western Blot Analysis of PDCD4 and PTEN
2
Protein Expression Analysis by Western Blot
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Protein concentration was determined by BCA protein assay kit (Bio-Rad, Italy) and cell lysates were subjected to SDS/PAGE (10% separation gel) and transferred onto a PVDF membrane. The following antibodies were used for Western blot: GAPDH Rabbit mAb antibody (Santa Cruz Biotechnology), PDCD4 Rabbit mAb antibodies (Cell Signal Technology, Danvers, MA, USA). We evaluated the relative expression of protein by Gel-pro Analyzer.
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