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2 protocols using ar 12

1

Antibiotic Preparation and Storage

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AR-12 (OSU-03012) was purchased from MedChemExpress (Monmouth Junction, NJ). Clarithromycin, amikacin, imipenem, cefoxitin, and tigecycline were purchased from Sigma-Aldrich (St. Louis, MO). Stock solutions of AR-12 (128 μg/ml) were dissolved in 32% DMSO (4 μg/ml in 1%). Clarithromycin (2,048 μg/ml) was prepared in 100% DMSO, and other antibiotics at 2,048 μg/ml were prepared in deionized water. All antibiotics were stored as aliquots at –20°C and serially diluted just prior to experimental use.
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2

Zebrafish Drug Treatment Protocol

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Dexamethasone (Sigma-Aldrich, D1756-25 MG), FK506 (InvivoGen, tlrl-fk5), rapamycin (Sigma-Aldrich, 44,532-U), cyclosporin A, metronidazole (Sigma-Aldrich, M1547-5 G), AR-12 (MedChem Express, HY-10547_5 mg), carbamazepine (MedChem Express, HY-B0246-100 mg), torin 1 (MedChem Express, HY-13003_5 mg), and voriconazole (Sigma-Aldrich, PZ0005-5 MG) were resuspended at 10 mg/ml in DMSO (Sigma-Aldrich, 154,938) and aliquoted in 100 µl stock concentrations at the recommended temperature for long-term storage for each drug. Working aliquots were kept at 4°C for a maximum of 4 weeks and added to the E3 water at the final concentration stated immediately after zebrafish inspection and before being transferred to individual wells.
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