C3H10T1/2 cells were seeded onto sterile coverslips. After 24 hr, cells were washed with PBS and fixed with 4% paraformaldehyde for 20 min. Cells were permeabilized with 0.1% Triton X-100 in PBS for 5 min, washed twice with PBS, and then blocked with 5% BSA in PBS for 20 min. Coverslips were rinsed with PBS and incubated overnight with mouse Alexa Fluor 488 anti-HA antibody (BioLegend, 901509) at a dilution of 1:100. Coverslips were then washed five times with PBS and samples stained with DAPI (4′,6-diamidino-2-phenylindole). Fluorescent images were obtained with a Zeiss fluorescence microscope.
Alexa fluor 488 anti ha antibody
Manufactured by BioLegend
The Alexa Fluor 488 anti-HA antibody is a fluorescently labeled monoclonal antibody that specifically binds to the hemagglutinin (HA) epitope tag. The Alexa Fluor 488 dye is conjugated to the antibody, allowing for the detection and visualization of HA-tagged proteins using fluorescence-based techniques.
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Lab products found in correlation
2 protocols using alexa fluor 488 anti ha antibody
1
Immunostaining of C3H10T1/2 Cells
2
Immunostaining of C3H10T1/2 Cells
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