Dmem f12 cell culture medium

Manufactured by Merck Group

Sourced in United States

DMEM/F12 is a cell culture medium used to support the growth and maintenance of various cell types in vitro. It is a nutritionally rich formulation that provides essential amino acids, vitamins, salts, and other components required for cell proliferation and survival. The medium is designed to maintain a stable pH and osmotic environment suitable for culturing cells.

Automatically generated - may contain errors

Lab products found in correlation

Trypsin, by Thermo Fisher Scientific (2 mentions) Sylgard 184, by Thermo Fisher Scientific (2 mentions) Pluronic f108, by Merck Group (2 mentions) Penicillin streptomycin, by Lonza (2 mentions) Skbr3, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Mcf 7, by Merck Group (1 mentions) Rpmi medium, by Merck Group (1 mentions) Dmem high glucose medium, by Merck Group (1 mentions) Foetal bovine serum (fbs), by Merck Group (1 mentions) Atcc htb 55, by American Type Culture Collection (1 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions) β galactosidase, by Merck Group (1 mentions) Sodium phosphate dibasic, by Thermo Fisher Scientific (1 mentions) Sodium phosphate monobasic, by Thermo Fisher Scientific (1 mentions) Pe anti ep cam, by BioLegend (1 mentions) Phosphate buffered saline (pbs), by Lonza (1 mentions)

Spelling variants of this product

DMEM/F12 (1116 citations) DMEM medium (495 citations) DMEM/F12 medium (260 citations) Cell culture medium (35 citations) DMEM culture medium (32 citations) F12 medium (27 citations) DMEM/F12 culture medium (15 citations) DMEM cell culture medium (8 citations) F12 culture medium (3 citations)

6 protocols using dmem f12 cell culture medium

Cell Culture Protocols for Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

MCF7, T47D, SKBR3 and HeLa cell lines were obtained from the American Type Culture Collection (Rockville). A4-HeLa was kindly provided by Dr. Tom Jovin and Dr. Donna Arndt-Jovin (Max Planck Institute for Biophysical Chemistry, Göttingen, Germany). MCF7 and T47D cell lines were routinely maintained in DMEM/F12 cell culture medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS, Internegocios, Córdoba, Argentina) in a humidified 5% CO2/air atmosphere. SKBR3 cell line was cultured in RPMI medium (Sigma-Aldrich) supplemented with 10% FBS. HeLa and A4-HeLa cell lines were maintained in DMEM high glucose medium (Sigma-Aldrich) supplemented with 10% FBS. Serial passages were carried out by treatment of 80% confluent monolayers with 0.25% trypsin (Invitrogen) and 0.02% EDTA in Ca2+-free and Mg2+-free phosphate-buffered saline (PBS).

Toscani A.M., Sampayo R.G., Barabas F.M., Fuentes F., Simian M, & Coluccio Leskow F. (2017). Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models. PLoS ONE, 12(3), e0174230.

+ Open protocol

+ Expand

Maintaining MCF7 and T47D Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

MCF7 and T47D cell lines were purchased from ATCC and regularly checked for mycoplasma. These cell lines were routinely maintained in DMEM/F12 cell culture medium (Sigma-Aldrich) supplemented with 10% FBS (Internegocios) and gentamicin, in a humidified 5% CO₂/air atmosphere. Serial passages were performed by treatment of 80% confluent monolayers with 0.25% trypsin (Invitrogen) and 0.02% EDTA in Ca²⁺-free and Mg²⁺-free PBS.

Sampayo R.G., Toscani A.M., Rubashkin M.G., Thi K., Masullo L.A., Violi I.L., Lakins J.N., Cáceres A., Hines W.C., Coluccio Leskow F., Stefani F.D., Chialvo D.R., Bissell M.J., Weaver V.M, & Simian M. (2018). Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells. The Journal of Cell Biology, 217(8), 2777-2798.

+ Open protocol

+ Expand

Microfluidic Dielectrophoresis Buffer Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The silicone elastomer and curing agent (Sylgard 184), bovine serum albumin (BSA) (biotech grade), and 0.25% Trypsin EDTA (1X) were purchased from Fisher Scientific (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The DMEM/F12 cell culture medium, dextrose (D-glucose), sucrose, Pluronic F-108 and 1.0 M Tris·HCl stock were obtained from Sigma-Aldrich, Inc. (St. Louis, MO, USA). All dilutions were conducted with Type 1 water (18.2 MΩ·cm). DEP buffer was comprised of 8.0% sucrose, 0.3% dextrose, and 0.1% BSA in 1.0 mM Tris buffer (pH 8.1, conductivity 72 µS/cm).

Banovetz J.T., Li M., Pagariya D., Kim S., Ganapathysubramanian B, & Anand R.K. (2019). Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths. Micromachines, 10(4), 271.

+ Open protocol

+ Expand

Calu-3 Cell Line Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Calu-3 cell line (ATCC^® HTB-55^™) was obtained from ATCC (Manassas, VA, USA). The cells were cultured in DMEM-F12 cell culture medium (Sigma Aldrich, St. Louis, MO, USA), supplemented with penicillin/streptomycin (1% v/v; Lonza) and foetal bovine serum (FBS; 10% v/v; Sigma Aldrich). The cell cultures were maintained in the incubator (Sanyo CO₂, Nagasaki, Japan) at 37 °C, 5% CO₂. The culture medium was changed every 2–3 days and the cells were passaged when 70–90% confluence was reached. The cells were detached from the flasks by trypsin/EDTA (0.25%/0.02% in phosphate-buffered saline, PBS, respectively). The cells were split in ratios from 1:3 to 1:6. The passages used for biocompatibility and permeation studies were 13–15 and 13, 19 and 23, respectively.

Nižić Nodilo L., Ugrina I., Špoljarić D., Amidžić Klarić D., Jakobušić Brala C., Perkušić M., Pepić I., Lovrić J., Saršon V., Safundžić Kučuk M., Zadravec D., Kalogjera L, & Hafner A. (2021). A Dry Powder Platform for Nose-to-Brain Delivery of Dexamethasone: Formulation Development and Nasal Deposition Studies. Pharmaceutics, 13(6), 795.

+ Open protocol

+ Expand

Optimizing β-Galactosidase Enzymatic Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The silicone elastomer and curing agent (Sylgard 184), bovine serum albumin (BSA) (biotech grade), 0.25% Trypsin EDTA (1X), sodium phosphate monobasic, sodium phosphate dibasic, fluorescein di-β-D-galactopyranoside (FDG), 2-mercaptoethanol, and 1-decyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ionic liquid were purchased from Fisher Scientific (Thermo Fisher Scientific, Inc., Waltham, MA). The DMEM/F12 cell culture medium, dextrose (D-glucose), sucrose, β-galactosidase, Pluronic F-108 were obtained from Sigma-Aldrich, Inc. (St. Louis, MO). PE/anti-EpCAM was obtained from BioLegend, San Diego, CA. All dilutions were conducted with Type 1 water (18.2 MΩ·cm). DEP buffer was comprised of 8.0% sucrose, 0.3% dextrose, and 0.1% BSA titrated to 60 μS/cm with 0.1 M phosphate buffer. The enzymatic assay buffer comprised 50 mM 2-mercaptoethanol and 1.0 mM fluorescein di-β-D-galactopyranoside (FDG) in 0.1 M phosphate buffer (pH 7.4). To optimize the cofactor concentration, magnesium chloride was added to distinct reaction volumes at 0.001 mM, 0.1 mM and 1.0 mM as described in the Results and Discussion section.

Banovetz J.T., Manimaran S., Schelske B, & Anand R.K. (2023). Parallel Dielectrophoretic Capture, Isolation, and Electrical Lysis of Individual Breast Cancer Cells to Assess Variability in Enzymatic Activity. Analytical chemistry, 95(20), 7880-7887.

+ Open protocol

+ Expand

Calu-3 Cell Line Biocompatibility Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

In order to examine in vitro biocompatibility and permeability of the formulations, Calu-3 cell line (ATCC^® HTB-55TM, ATCC, Manassas, VA, USA) was used. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM-F12) cell culture medium (Sigma Aldrich, Burlington, MA, USA) containing penicillin/streptomycin (1% v/v; Lonza, Basel, Switzerland) and fetal bovine serum (FBS; 1% v/v, Sigma Aldrich, Burlington, MA, USA). The cell cultures were maintained at 95% humidity and 37 °C in an atmosphere of 5% CO₂ (Sanyo CO₂ incubator, Osaka, Japan). The medium was changed every 2 days, and the cells were passaged when they reached 70–90% confluence, according to the ATCC recommended protocol. The detachment of the cells from the flasks was performed using a mixture of trypsin (0.25%) and EDTA (0.02%) solutions in phosphate-buffered saline (PBS; Lonza, Basel, Switzerland).

Perkušić M., Nižić Nodilo L., Ugrina I., Špoljarić D., Jakobušić Brala C., Pepić I., Lovrić J., Safundžić Kučuk M., Trenkel M., Scherließ R., Zadravec D., Kalogjera L, & Hafner A. (2023). Chitosan-Based Thermogelling System for Nose-to-Brain Donepezil Delivery: Optimising Formulation Properties and Nasal Deposition Profile. Pharmaceutics, 15(6), 1660.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!