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Il 6 assay kit

Manufactured by R&D Systems
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Sourced in United States

The IL-6 Assay Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of Interleukin-6 (IL-6) levels in cell culture supernatants, serum, and plasma. The kit includes a 96-well microplate pre-coated with an antibody specific for IL-6, standards, and reagents necessary to perform the assay.

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Lab products found in correlation

Synergy h1, by Agilent Technologies (1 mentions) Microplate reader, by Bio-Rad (1 mentions)

Close spelling variants of this product

ELISA-based assay kits for the detection of IL-6, Enzyme-linked immunosorbent assay (ELISA) kits for IL-6 Enzyme-linked immunosorbent assay (ELISA) kits for IL-6 and TNF-α IL-6 enzyme-linked immunosorbent assay kits IL-6 enzyme-linked immunosorbent assay (ELISA) kits Human IL-6 Quantikine enzyme-linked immunosorbent assay (ELISA) Kit Enzyme-linked immunosorbent assay kit for porcine IL-6 Rat IL-6 Quantikine ELISA assay kit Human IL-6 Quantikine enzyme-linked immunosorbent assay kit IL-6 kits

2 protocols using il 6 assay kit

1

Cytokine Quantification Assay Protocol

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IL-1b assay kit (R&D Systems), IL-6 assay kit (R&D Systems), TNF-α assay kit (R&D Systems), and IL-23a assay kit (Abcam) were used according to the kit instructions. OD was detected using Synergy H1 (Biotek) recorded at 450 nm.
Wang J., Wu S., Liu L., Pang Y., Li Z, & Mu H. (2022). HDAC5-Mediated Acetylation of p100 Suppresses Its Processing. International Dental Journal, 73(3), 387-394.
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2

Measuring Cytokine Levels in Disease

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The levels of IL-1 and IL-6 in peripheral blood in disease group and control group were measured through enzyme-linked immunosorbent assay using a IL-1 assay kit and a IL-6 assay kit (R&D, Minneapolis, MN, USA) in strict accordance with the instructions of the kits. Three replicates were set for each well. After the assay was completed, the absorbance value was read at a wavelength of 450 nm using a microplate reader (Bio-Rad, Hercules, CA, USA), and the levels of IL-1 and IL-6 in disease group and control group were obtained after conversion based on standard curves.
Liu N., Liu C., Wu Y, & Li H. (2024). Correlations of IL-1 and IL-6 Gene polymorphisms with hypertrophic cardiomyopathy. Cellular and molecular biology (Noisy-le-Grand, France), 70(4).
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