Luria bertani agar

PCL (Mw = 80,000), PEP essential oil (purified by triple-distillation), standard menthol, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (Darmstadt, Germany). Glacial acetic acid (GAA, VWR, Darmstadt, Germany) was used as a solvent. Phosphate-buffered saline (PBS, biotech grade, pH 7.4) and dichloromethane (DCM) were obtained from VWR (Darmstadt, Germany). The microorganism strains of S. aureus (ATCC25923), and E. coli (ATCC25922) were used in our laboratory. Luria-Bertani (LB) agar and lysogeny broth (LB) medium were supplied by Carl Roth GmbH (Karlsruhe, Germany). Dulbecco's modified Eagle‘s medium (DMEM), penicillin/streptomycin (PS), and trypsin/EDTA were purchased from Thermo Scientific (Schwerte, Germany). NHDF cell line was obtained from Translation Research Center (TRC), Erlangen. All reagents and solvents were of analytical grade.

Tobramycin (Tob, molecular weight (Mw) 467.5 Da), ciprofloxacin (Cipro, Mw 331.3 Da), itaconic acid (IA, Mw 130.1 Da), peptone, Tween20 and casein were obtained from Merck. Salts and organic compounds of analytical grade (NH₄Cl, KCl, tris-HCl, chloroform, glycerol, glucose, formic acid and MgSO₄·7H₂O) were obtained from Merck and VWR. Yeast extract was obtained from Fluka. Bacto™ Tryptone were obtained from BD Biosciences. Luria Bertani (LB) agar was obtained from Carl Roth. Gibco^® PBS was obtained from Life Technologies. Purified water was produced by Milli-Q water purification system (Merk Millipore, Billerica, MA, USA)
The Pseudomonas aeruginosa strain PA14 wild type (wt) was obtained from ATCC or DSMZ, the German Collection of Microorganism and Cell Cultures GmbH (PA14 = DSMZ19882), stored in glycerol stocks at −80 °C. Bacteria were cultured in a minimal proteose peptone glucose ammonium salt (PPGAS) medium. PPGAS was composed of 1 g/L NH₄Cl, 1.5 g/L KCl, 19 g/L Tris-HCl, 10 g/L peptone, 5 g/L glucose and 0.1 g/L MgSO4·7H₂O. The medium was adjusted to pH 7.2 ± 0.2 and sterilized before use. Agar solution was composed of 15.5 g/L LB agar, 10 g/L peptone, 5 g/L NaCl and 5 g/L yeast extract, the solution was sterilized before being plated.

All chemicals were purchased from Sigma-Aldrich unless otherwise specified. Tetrahydrofuran (HPLC grade) (THF), ethanol, absolute (HPLC grade) (EtOH), ethyl acetate (analytical grade reagent), and Formic Acid Optima LCMS were purchased from Fisher Scientific. Acetonitrile and methanol (MeOH) were obtained from VWR Chemicals. Yeast extract was obtained from Fluka. Bacto™ Tryptone was obtained from BD Biosciences. Luria Bertani (LB) agar was obtained from Carl Roth. Gibco® HBSS (1x) Hanks' Balanced Salt Solution and Gibco® PBS was obtained from Life Technologies. Purified water was prepared by a Milli-Q water purification system (Merck Millipore, Billerica, MA, USA) (called water in the manuscript).