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Perfluorooctanoic acid pfoa

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Perfluorooctanoic acid (PFOA) is a synthetic chemical compound used in the manufacture of various industrial and consumer products. It is a colorless, odorless, and water-soluble substance that exhibits unique properties, such as resistance to heat, oil, stains, grease, and water. PFOA is commonly used as a processing aid in the production of fluoropolymers, which are found in a wide range of applications, including non-stick cookware, stain-resistant textiles, and firefighting foams.

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Lab products found in correlation

Cyclosporine a, by Merck Group (1 mentions) Verteporfin, by Bio-Techne (1 mentions) Myristic acid, by Tokyo Chemical Industry (1 mentions) Palmitic acid, by Tokyo Chemical Industry (1 mentions) Stearic acid, by Tokyo Chemical Industry (1 mentions) Docosanoic acid, by Merck Group (1 mentions) Decanoic acid, by Merck Group (1 mentions) Lauric acid, by Tokyo Chemical Industry (1 mentions) Tetracosanoic acid, by Merck Group (1 mentions) Docosapentaenoic acid, by Merck Group (1 mentions) Arachidic acid, by Merck Group (1 mentions) 9 decenoic acid, by Merck Group (1 mentions) Lc ms ms grade acetonitrile, by Merck Group (1 mentions) Hplc grade hexane, by Merck Group (1 mentions) Oleic acid, by Merck Group (1 mentions) Potassium persulfate, by Thermo Fisher Scientific (1 mentions) Perfluorobutanoic acid pfba, by Merck Group (1 mentions) Methanol optima, by Thermo Fisher Scientific (1 mentions) Hplc grade water, by Thermo Fisher Scientific (1 mentions) Concentrated sulfuric acid, by Avantor (1 mentions)

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Perfluorooctanoic acid (24 citations)

12 protocols using perfluorooctanoic acid pfoa

1

Procurement of PFOA, PFOS, and CSA

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Perfluorooctanoic acid (PFOA) and cyclosporine A (CSA) were purchased from Sigma-Aldrich (Taufkirchen, Germany) and perfluorooctanesulfonic acid (PFOS) was obtained from ABCR GmbH (Karlsruhe, Germany) in the highest available purity.
Behr A.C., Kwiatkowski A., Ståhlman M., Schmidt F.F., Luckert C., Braeuning A, & Buhrke T. (2020). Impairment of bile acid metabolism by perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) in human HepaRG hepatoma cells. Archives of Toxicology, 94(5), 1673-1686.
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2

Cytotoxicity Evaluation of Chemical Compounds

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Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12), fetal bovine serum (FBS), and penicillin-streptomycin (P/S) were obtained from Cytiva (Global Life Science Solutions, Marlborough, MA). 3–4(4,5-dimethyl-2-yl)2,5-diphenyl-2 H-tetrazolium bromide (MTT), goat serum, and perfluorooctanoic acid (PFOA; purity 95%) were purchased from Sigma-Aldrich. TrypLE™ Express Enzyme, bovine serum albumin (BSA), dimethyl sulfoxide (DMSO), tween-20, Triton X-100 were purchased from Fischer Scientific. Verteporfin was purchased from Tocris (Bio-Techne, Minneapolis, MN).
Morgan D., Mahe C., Mayanja B, & Whitworth J.A. (2002). Progression to symptomatic disease in people infected with HIV-1 in rural Uganda: prospective cohort study. BMJ : British Medical Journal, 324(7331), 193-197.
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3

Fatty Acid Quantification by LC-MS/MS

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The following chemicals were purchased from Tokyo Chemical Industry (Tokyo, Japan): n-octanoic acid (C8:0), lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), cis-9-hexadecenoic acid (C16:1), stearic acid (C18:0), γ-linolenic acid (C18:3), cis-5,8,11,14,17-eicosapentaenoic acid (EPA; C20:5) and arachidonic acid (C20:4). Decanoic acid (C10:0), 9-decenoic acid (C10:1), oleic acid (C18:1), arachidic acid (C20:0), all-cis-7,10,13,16,19-docosapentaenoic acid (DPA, C22:5), docosanoic acid (C22:0), tetracosanoic acid (C24:0), hexacosanoic acid (C26:0), DAABD-AE, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), 4-(dimethylamino) pyridine (DMAP) and perfluorooctanoic acid (PFOA) were purchased from Sigma-Aldrich (Taufkirchen, Germany). The following deuterium or 13C labeled analogs used as IS were purchased from Cambridge Isotopes Laboratories (Tewksbury, MA, USA): 13C4-C8:0, d3-C10:0, d3-C12:0, d3-C14:0, d4-C16:0 and d3-C18:0. PRA, PHA, d3-PRA, d3-PHA, d4-C22:0, d4-C24:0, and d4-C26:0 were obtained from Dr. H. J. Ten Brink (Vrije Universiteit Medical Center, Amsterdam, The Netherlands). LC-MS/MS grade acetonitrile and water were purchased from Merck (Darmstadt, Germany). Merck also supplied us with HPLC grade hexane, toluene, and heptane.
Aidaros A.A., Sharma C., Langhans C.D., G. Okun J., Hoffmann G.F., Dasouki M., Chakraborty P., Aljasmi F, & Y. Al-Dirbashi O. (2020). Targeted Metabolomic Profiling of Total Fatty Acids in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry. Metabolites, 10(10), 400.
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4

Perfluoroalkyl Compound Preparation and Dosing

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Three perfluoroalkyl compounds were used in the experiments: perfluorooctanoic acid (PFOA) of 95% purity (Cat. no. 171468) was purchased from Sigma-Aldrich, USA, perfluorooctane sulphonic acid (PFOS) with 97% purity (Cat. no. 6164-3-08) and GenX (also called 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy) propanoic acid/Undecafluoro-2-methyl-3-oxahexanoic acid) of 97% purity (CAS no. 13252-13-6), were purchased from Synquest Laboratories Inc., USA. All of the compounds were constituted in Dimethyl Sulfoxide (DMSO, Cat no. 25-950-CQC, Corning Inc., New York, NY, USA) to make one molar stock solution. The stock solutions were then spiked into the cell culture medium to make 10 to 1000 μM working concentrations such that the final concentration of DMSO in the treatment medium and vehicle control was ≤0.1%. As discussed below with the cell viability assay, 0.1% of DMSO is non-toxic to cells.
Jabeen M., Fayyaz M, & Irudayaraj J. (2020). Epigenetic Modifications, and Alterations in Cell Cycle and Apoptosis Pathway in A549 Lung Carcinoma Cell Line upon Exposure to Perfluoroalkyl Substances. Toxics, 8(4), 112.
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5

PFCA Adsorption Behavior in Activated Carbon

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Perfluorooctanoic acid (PFOA) and perfluorobutanoic acid (PFBA) were purchased from Sigma-Aldrich (USA). Powdered activated carbon (PAC, Type TOG, 50×200, Calgon Corporation, Pittsburg, PA) was used after washing with water and methanol twice. All analytical standards and internal standards were purchased from Wellington Laboratories. Potassium persulfate, HPLC grade water and Optima methanol were purchased from Fisher Scientific. All other chemicals and solvents were obtained from Fisher Scientific or Sigma Aldrich. Falcon cell culture flasks, which consist of polystyrene, were used in all experiments. These flasks were chosen because they exhibited the smallest losses of PFCAs and were stable at elevated pH values and temperatures. All flasks were kept in the dark during the experiments.
Sun B., Ma J, & Sedlak D.L. (2016). Chemisorption of Perfluorooctanoic Acid on Powdered Activated Carbon Initiated by Persulfate in Aqueous Solution. Environmental science & technology, 50(14), 7618-7624.
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6

Sulfonation of Polysulfone and Phosphorene Synthesis

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Perfluorooctanoic acid (PFOA) was purchased from Sigma Aldrich (St Louis, MO, USA). For the sulfonation reaction, polysulfone (PSf), N-methyl pyrrolidone (NMP), poly ether ether ketone (PEEK), and concentrated sulfuric acid were purchased from VWR (Radnor, PA, USA). Black phosphorus used for the synthesis of phosphorene was purchased from Smart Elements (Vienna, Austria).
Eke J., Banks L., Mottaleb M.A., Morris A.J., Tsyusko O.V, & Escobar I.C. (2020). Dual-Functional Phosphorene Nanocomposite Membranes for the Treatment of Perfluorinated Water: An Investigation of Perfluorooctanoic Acid Removal via Filtration Combined with Ultraviolet Irradiation or Oxygenation. Membranes, 11(1), 18.
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7

PFECA Characterization and Quantification

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Perfluoroethercarboxylic acids (PFECA) including perfluoro (4methoxy butanoic) acid (PFMOBA), perfluoro-3,6,9trioxatridecanoic acid (PFO3TDA), perfluoro-3,6,9-trioxadecanoic acid (PFO3DA), perfluoro (2-methyl-3-oxahexanoic) acid (trademarked commercially under the name "GenX"), perfluoro-3,6dioxaheptanoic acid (PFO2HpA), 4-(heptafluoroisopropoxy)hexafluorobutanoic acid (PFDMMOBA) and Perfluoro-3,6-dioxadecanoic acid (PFO2DA) were purchased from SynQuest Laboratories (Dallas, TX U.S.A.). Perfluorooctanoic acid (PFOA), and all other chemicals (i.e., deuterated phosphate buffer and reference standard for NMR), were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). Stock solutions of PFECAs and PFOA were prepared with deionized water in polypropropylene tubes (to prevent adsorption to glass; Shafique et al., 2017) , and were sonicated until complete dissolution of the compounds was achieved. All chemicals were utilized without further purification.
Gebreab K.Y., Eeza M.N.H., Bai T., Zuberi Z., Matysik J., O'Shea K.E., Alia A, & Berry J.P. (2020). Comparative toxicometabolomics of perfluorooctanoic acid (PFOA) and next-generation perfluoroalkyl substances. Environmental pollution (Barking, Essex : 1987), 265(Pt A).
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8

Adsorption of PFOA on Rice Husk

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RHs from white rice were obtained from a local market in Bangkok, Thailand. Perfluorooctanoic acid (PFOA) was purchased from Sigma Aldrich with a molecular weight of 414.07 g/mol. 85% phosphoric acid (H3PO4, PA) was procured from Anapure. Hydrochloric acid (HCl) from QRëC and sodium hydroxide (NaOH) from Sigma Aldrich were used to control the pH solution. 99.9% sodium chloride (NaCl) was purchased from Gammaco for pH point of zero charge (pHpzc) determination. A glass fibers syringe filter with a 2.0 µm pore size was purchased from Merck Millipore. 0.1 N sodium thiosulfate (Na2S2O3) with a molecular weight of 248.18 g/mol was obtained from KemAus for the titration method. The corn starch for the starch solution was acquired from a nearby supermarket. Wijs iodine solution was procured from RCI Labscan. All required solutions were prepared using deionized (DI) water generated in the laboratory. Ultra-high purity (UHP) He and O2 gas cylinders were procured from Alternative Chemical.
Sahara T., Wongsawaeng D., Ngaosuwan K., Kiatkittipong W., Hosemann P, & Assabumrungrat S. (2023). Highly effective removal of perfluorooctanoic acid (PFOA) in water with DBD-plasma-enhanced rice husks. Scientific Reports, 13, 13210.
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9

Electrochemical Biosensor Protocol for PFOA/PFOS Detection

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Pyrrole-2-carboxylic acid (99%) (Py-2-COOH), perfluorooctanoic acid (PFOA, !96%), perfluorooctanesulfonic acid potassium salt (PFOS, !98%) and hydroxylamine were purchased from Sigma-Aldrich Ltd (Belgium). 1-ethyl-3-(3-dimethylamino) propyl carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (NHS) were purchased from TCI (Europe). Highly purified delipidated human serum albumin (hSA) was obtained following the protocol reported in [36] . All the other reagents were of analytical grade and used as received. The 0.1 M phosphate buffer saline pH 7.4 with 0.01 NaCl was prepared by mixing stock solutions of 0.1 M NaH 2 PO 4 and 0.1 M Na 2 HPO 4 , purchased form Sigma Aldrich. All aqueous solutions were prepared using MilliQ water (R > 18 MO cm). Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measurements were performed using an Autolab potentiostat/galvanostat (PGSTAT 302N, ECOCHEMIE, The Netherlands) controlled by NOVA 1.1 software. Disposable graphite screen-printed electrodes (G-SPE) consisting of a graphite working (3 mm diameter) and counter electrode and a silver pseudo reference electrode were purchased from Italsens (Florence, Italy).
Moro G., Bottari F., Liberi S., Covaceuszach S., Cassetta A., Angelini A., De Wael K, & Moretto L.M. (2020). Covalent immobilization of delipidated human serum albumin on poly(pyrrole-2-carboxylic) acid film for the impedimetric detection of perfluorooctanoic acid. Bioelectrochemistry (Amsterdam, Netherlands), 134.
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10

PFOA Proliferation Assay Protocol

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Perfluorooctanoic acid (PFOA, Cat# 171468) was purchased from Sigma Chemical Company (St. Louis, MO). A cell proliferation assay kit (Cat# G5421) was obtained from Promega (Madison, WI). Fetal bovine serum (FBS) was obtained from Hyclone (Seattle, WA).
Raber J., Wong D., Buttini M., Orth M., Bellosta S., Pitas R.E., Mahley R.W, & Mucke L. (1998). Isoform-specific effects of human apolipoprotein E on brain function revealed in ApoE knockout mice: Increased susceptibility of females. Proceedings of the National Academy of Sciences of the United States of America, 95(18), 10914-10919.
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